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Spontaneous Accumulation of Globotriaosylceramide (Gb3) in Proximal Renal Tubules in an ICR Mouse.

Mutsuga M, Asaoka Y, Togashi Y, Imura N, Miyoshi T, Miyamoto Y - J Toxicol Pathol (2013)

Bottom Line: The contents of vacuoles were positively stained with periodic acid-Schiff (PAS) and Sudan black, and the membranes were positive on immunohistochemical staining for lysosomal-associated membrane protein-2 (LAMP-2), a marker of lysosomal membrane.Electron microscopy revealed electron-dense lamellar bodies in the proximal tubular epithelial cells.When we performed immunohistochemical staining for Gb3, the contents of vacuoles were positively stained.

View Article: PubMed Central - PubMed

Affiliation: Toxicology and Pharmacokinetics Laboratories, Pharmaceutical Research Laboratories, Toray Industries, Inc., 6-10-1 Tebiro,Kamakura, Kanagawa 248-8555, Japan.

ABSTRACT
This report describes spontaneous cytoplasmic vacuolation in the proximal renal tubules of a 7-week-old male ICR [Crlj:CD1(ICR)] mouse. The contents of vacuoles were positively stained with periodic acid-Schiff (PAS) and Sudan black, and the membranes were positive on immunohistochemical staining for lysosomal-associated membrane protein-2 (LAMP-2), a marker of lysosomal membrane. Electron microscopy revealed electron-dense lamellar bodies in the proximal tubular epithelial cells. These histopathological features are similar to those in α-galactosidase A-deficient mice, in which globotriaosylceramide (Gb3), a glycosphingolipid, accumulates in lysosomes. When we performed immunohistochemical staining for Gb3, the contents of vacuoles were positively stained. From these results, spontaneous cytoplasmic vacuolation in the proximal renal tubules in the mouse was identified as lysosomal accumulation of Gb3.

No MeSH data available.


Electron microscopy in the proximal renal tubules. Electron-dense lamellar bodies are seen in the proximal tubular epithelial cells.
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fig_003: Electron microscopy in the proximal renal tubules. Electron-dense lamellar bodies are seen in the proximal tubular epithelial cells.

Mentions: PAS staining, Sudan black staining, and immunohistochemical staining for LAMP-2 in the proximal renal tubules. The contents of vacuoles are positive for PAS and Sudan black staining (A, B), and the membranes are positive for LAMP-2 immunohistochemical staining (C). A: PAS staining. B: Sudan black staining. C: immunohistochemical staining for LAMP-2.


Spontaneous Accumulation of Globotriaosylceramide (Gb3) in Proximal Renal Tubules in an ICR Mouse.

Mutsuga M, Asaoka Y, Togashi Y, Imura N, Miyoshi T, Miyamoto Y - J Toxicol Pathol (2013)

Electron microscopy in the proximal renal tubules. Electron-dense lamellar bodies are seen in the proximal tubular epithelial cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921927&req=5

fig_003: Electron microscopy in the proximal renal tubules. Electron-dense lamellar bodies are seen in the proximal tubular epithelial cells.
Mentions: PAS staining, Sudan black staining, and immunohistochemical staining for LAMP-2 in the proximal renal tubules. The contents of vacuoles are positive for PAS and Sudan black staining (A, B), and the membranes are positive for LAMP-2 immunohistochemical staining (C). A: PAS staining. B: Sudan black staining. C: immunohistochemical staining for LAMP-2.

Bottom Line: The contents of vacuoles were positively stained with periodic acid-Schiff (PAS) and Sudan black, and the membranes were positive on immunohistochemical staining for lysosomal-associated membrane protein-2 (LAMP-2), a marker of lysosomal membrane.Electron microscopy revealed electron-dense lamellar bodies in the proximal tubular epithelial cells.When we performed immunohistochemical staining for Gb3, the contents of vacuoles were positively stained.

View Article: PubMed Central - PubMed

Affiliation: Toxicology and Pharmacokinetics Laboratories, Pharmaceutical Research Laboratories, Toray Industries, Inc., 6-10-1 Tebiro,Kamakura, Kanagawa 248-8555, Japan.

ABSTRACT
This report describes spontaneous cytoplasmic vacuolation in the proximal renal tubules of a 7-week-old male ICR [Crlj:CD1(ICR)] mouse. The contents of vacuoles were positively stained with periodic acid-Schiff (PAS) and Sudan black, and the membranes were positive on immunohistochemical staining for lysosomal-associated membrane protein-2 (LAMP-2), a marker of lysosomal membrane. Electron microscopy revealed electron-dense lamellar bodies in the proximal tubular epithelial cells. These histopathological features are similar to those in α-galactosidase A-deficient mice, in which globotriaosylceramide (Gb3), a glycosphingolipid, accumulates in lysosomes. When we performed immunohistochemical staining for Gb3, the contents of vacuoles were positively stained. From these results, spontaneous cytoplasmic vacuolation in the proximal renal tubules in the mouse was identified as lysosomal accumulation of Gb3.

No MeSH data available.