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Granulomatous nephritis consistent with malakoplakia in a cynomolgus monkey.

Taketa Y, Inomata A, Sonoda J, Hayakawa K, Nakano-Ito K, Ohta E, Seki Y, Goto A, Hosokawa S - J Toxicol Pathol (2013)

Bottom Line: Gross examination showed that the kidney was markedly enlarged and adhered to the surrounding organs.Histology showed that there was diffuse interstitial infiltration of histiocytes with abundant foamy eosinophilic cytoplasm resembling von Hansemann cells, PAS-positive granular cytoplasm and occasional PAS- and iron-positive intracellular small inclusion bodies.Electron microscopy showed that these histiocytes contained abundant lysosomes and phagolysosomes but no obvious Michaelis-Gutmann bodies.

View Article: PubMed Central - PubMed

Affiliation: Tsukuba Drug Safety, Global Drug Safety, Biopharmaceutical Assessments Core Function Unit, Eisai Product Creation Systems, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan.

ABSTRACT
Malakoplakia is a rare form of chronic granulomatous inflammation in mammals, and usually affects the urinary tract in humans. In this report, we present a case of granulomatous nephritis consistent with malakoplakia in a 4-year-old male cynomolgus monkey. Gross examination showed that the kidney was markedly enlarged and adhered to the surrounding organs. Histology showed that there was diffuse interstitial infiltration of histiocytes with abundant foamy eosinophilic cytoplasm resembling von Hansemann cells, PAS-positive granular cytoplasm and occasional PAS- and iron-positive intracellular small inclusion bodies. Electron microscopy showed that these histiocytes contained abundant lysosomes and phagolysosomes but no obvious Michaelis-Gutmann bodies. Based on these findings, a diagnosis of granulomatous nephritis consistent with early malakoplakia was made. This is the first report in a monkey of a renal lesion consistent with malakoplakia.

No MeSH data available.


Related in: MedlinePlus

H&E (a), PAS (b) and Berlin blue (c) stainings and immunohistochemistry for CD204 (d) at highmagnification. H&E staining shows the large-sized foamy cells with abundant fine eosinophilic cytoplasmic granules and eosinophilic inclusion bodies (arrowheads) (a). PAS-positive granular cytoplasm and PAS-positive round to irregularly shaped inclusion bodies (arrowheads) (b). Inclusion bodies are also positive for iron staining (Berlin blue, arrowheads) (c). The eosinophilic foamy cells are positive for CD204 immunostaining (d). Bars = 20 μm.
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fig_002: H&E (a), PAS (b) and Berlin blue (c) stainings and immunohistochemistry for CD204 (d) at highmagnification. H&E staining shows the large-sized foamy cells with abundant fine eosinophilic cytoplasmic granules and eosinophilic inclusion bodies (arrowheads) (a). PAS-positive granular cytoplasm and PAS-positive round to irregularly shaped inclusion bodies (arrowheads) (b). Inclusion bodies are also positive for iron staining (Berlin blue, arrowheads) (c). The eosinophilic foamy cells are positive for CD204 immunostaining (d). Bars = 20 μm.

Mentions: Low-magnification image of the renal lesion. The lesion consists of diffuse interstitial infiltration of large and eosinophilic cells and inflammatory cells, occasional degenerative or regenerative renal tubules and intact glomeruli (H&E staining). Bar = 100 μm.


Granulomatous nephritis consistent with malakoplakia in a cynomolgus monkey.

Taketa Y, Inomata A, Sonoda J, Hayakawa K, Nakano-Ito K, Ohta E, Seki Y, Goto A, Hosokawa S - J Toxicol Pathol (2013)

H&E (a), PAS (b) and Berlin blue (c) stainings and immunohistochemistry for CD204 (d) at highmagnification. H&E staining shows the large-sized foamy cells with abundant fine eosinophilic cytoplasmic granules and eosinophilic inclusion bodies (arrowheads) (a). PAS-positive granular cytoplasm and PAS-positive round to irregularly shaped inclusion bodies (arrowheads) (b). Inclusion bodies are also positive for iron staining (Berlin blue, arrowheads) (c). The eosinophilic foamy cells are positive for CD204 immunostaining (d). Bars = 20 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921925&req=5

fig_002: H&E (a), PAS (b) and Berlin blue (c) stainings and immunohistochemistry for CD204 (d) at highmagnification. H&E staining shows the large-sized foamy cells with abundant fine eosinophilic cytoplasmic granules and eosinophilic inclusion bodies (arrowheads) (a). PAS-positive granular cytoplasm and PAS-positive round to irregularly shaped inclusion bodies (arrowheads) (b). Inclusion bodies are also positive for iron staining (Berlin blue, arrowheads) (c). The eosinophilic foamy cells are positive for CD204 immunostaining (d). Bars = 20 μm.
Mentions: Low-magnification image of the renal lesion. The lesion consists of diffuse interstitial infiltration of large and eosinophilic cells and inflammatory cells, occasional degenerative or regenerative renal tubules and intact glomeruli (H&E staining). Bar = 100 μm.

Bottom Line: Gross examination showed that the kidney was markedly enlarged and adhered to the surrounding organs.Histology showed that there was diffuse interstitial infiltration of histiocytes with abundant foamy eosinophilic cytoplasm resembling von Hansemann cells, PAS-positive granular cytoplasm and occasional PAS- and iron-positive intracellular small inclusion bodies.Electron microscopy showed that these histiocytes contained abundant lysosomes and phagolysosomes but no obvious Michaelis-Gutmann bodies.

View Article: PubMed Central - PubMed

Affiliation: Tsukuba Drug Safety, Global Drug Safety, Biopharmaceutical Assessments Core Function Unit, Eisai Product Creation Systems, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan.

ABSTRACT
Malakoplakia is a rare form of chronic granulomatous inflammation in mammals, and usually affects the urinary tract in humans. In this report, we present a case of granulomatous nephritis consistent with malakoplakia in a 4-year-old male cynomolgus monkey. Gross examination showed that the kidney was markedly enlarged and adhered to the surrounding organs. Histology showed that there was diffuse interstitial infiltration of histiocytes with abundant foamy eosinophilic cytoplasm resembling von Hansemann cells, PAS-positive granular cytoplasm and occasional PAS- and iron-positive intracellular small inclusion bodies. Electron microscopy showed that these histiocytes contained abundant lysosomes and phagolysosomes but no obvious Michaelis-Gutmann bodies. Based on these findings, a diagnosis of granulomatous nephritis consistent with early malakoplakia was made. This is the first report in a monkey of a renal lesion consistent with malakoplakia.

No MeSH data available.


Related in: MedlinePlus