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No Promoting Effect of Ethyl Tertiary-butyl Ether (ETBE) on Rat Urinary Bladder Carcinogenesis Initiated with N-Butyl-N-(4-hydroxybutyl)nitrosamine.

Hagiwara A, Imai N, Doi Y, Suguro M, Kawabe M, Furukawa F, Nagano K, Fukushima S - J Toxicol Pathol (2013)

Bottom Line: No statistically significant differences in incidences of preneoplastic lesions, papillomas, and carcinomas of the urinary bladder were evident in rats treated with 100-1000 mg/kg/day ETBE as compared with control values.However, papillomatosis of the urinary bladder was found in 4 out of 30 rats (13%) in the group given 1000 mg/kg/day ETBE, and soft stones in the urinary bladder were found in 3 out of these 4 rats.The results thus demonstrated that ETBE did not exert promotional activity on urinary bladder carcinogenesis.

View Article: PubMed Central - PubMed

Affiliation: DIMS Institute of Medical Science, Inc., 64 Goura, Nishiazai, Azai-cho, Ichinomiya, Aichi 491-0113, Japan.

ABSTRACT
The effects of ethyl tertiary-butyl ether (ETBE) on two-stage urinary bladder carcinogenesis in male F344 rats initiated with N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) were investigated at various dose levels with regard to possible promoting activity. Groups of 30 rats were given drinking water containing 500 ppm BBN, as an initiator, for 4 weeks and starting one week thereafter received ETBE by gavage (daily, 7 days/week) at dose levels of 0 (control), 100, 300, 500 or 1000 mg/kg/day until experimental week 36. No statistically significant differences in incidences of preneoplastic lesions, papillomas, and carcinomas of the urinary bladder were evident in rats treated with 100-1000 mg/kg/day ETBE as compared with control values. Furthermore, the average numbers of preneoplastic or neoplastic lesions per unit length of basement membrane in rats given 100-1000 mg/kg/day ETBE were also comparable to control values. However, papillomatosis of the urinary bladder was found in 4 out of 30 rats (13%) in the group given 1000 mg/kg/day ETBE, and soft stones in the urinary bladder were found in 3 out of these 4 rats. The results thus demonstrated that ETBE did not exert promotional activity on urinary bladder carcinogenesis. However, papillomatosis of the urinary bladder developed in small numbers of the rats given ETBE at 1000 mg/kg/day but not in rats given 500 mg/kg/day or lower doses.

No MeSH data available.


Related in: MedlinePlus

Experimental design. Animals were given drinking water containing 500 ppm BBM for 4 weeks. One week after the end of week 4, the animals were administered ETBE at dose levels of 0 (control), 100, 300, 500 and 1000 mg/kg/day by gavage (daily, 7 days/week) for 31 weeks from week 6 to 36. All survivors were euthanized at week 37.
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fig_001: Experimental design. Animals were given drinking water containing 500 ppm BBM for 4 weeks. One week after the end of week 4, the animals were administered ETBE at dose levels of 0 (control), 100, 300, 500 and 1000 mg/kg/day by gavage (daily, 7 days/week) for 31 weeks from week 6 to 36. All survivors were euthanized at week 37.

Mentions: An outline of the experimental design for the present study is shown in Fig. 1Fig. 1.


No Promoting Effect of Ethyl Tertiary-butyl Ether (ETBE) on Rat Urinary Bladder Carcinogenesis Initiated with N-Butyl-N-(4-hydroxybutyl)nitrosamine.

Hagiwara A, Imai N, Doi Y, Suguro M, Kawabe M, Furukawa F, Nagano K, Fukushima S - J Toxicol Pathol (2013)

Experimental design. Animals were given drinking water containing 500 ppm BBM for 4 weeks. One week after the end of week 4, the animals were administered ETBE at dose levels of 0 (control), 100, 300, 500 and 1000 mg/kg/day by gavage (daily, 7 days/week) for 31 weeks from week 6 to 36. All survivors were euthanized at week 37.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921917&req=5

fig_001: Experimental design. Animals were given drinking water containing 500 ppm BBM for 4 weeks. One week after the end of week 4, the animals were administered ETBE at dose levels of 0 (control), 100, 300, 500 and 1000 mg/kg/day by gavage (daily, 7 days/week) for 31 weeks from week 6 to 36. All survivors were euthanized at week 37.
Mentions: An outline of the experimental design for the present study is shown in Fig. 1Fig. 1.

Bottom Line: No statistically significant differences in incidences of preneoplastic lesions, papillomas, and carcinomas of the urinary bladder were evident in rats treated with 100-1000 mg/kg/day ETBE as compared with control values.However, papillomatosis of the urinary bladder was found in 4 out of 30 rats (13%) in the group given 1000 mg/kg/day ETBE, and soft stones in the urinary bladder were found in 3 out of these 4 rats.The results thus demonstrated that ETBE did not exert promotional activity on urinary bladder carcinogenesis.

View Article: PubMed Central - PubMed

Affiliation: DIMS Institute of Medical Science, Inc., 64 Goura, Nishiazai, Azai-cho, Ichinomiya, Aichi 491-0113, Japan.

ABSTRACT
The effects of ethyl tertiary-butyl ether (ETBE) on two-stage urinary bladder carcinogenesis in male F344 rats initiated with N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) were investigated at various dose levels with regard to possible promoting activity. Groups of 30 rats were given drinking water containing 500 ppm BBN, as an initiator, for 4 weeks and starting one week thereafter received ETBE by gavage (daily, 7 days/week) at dose levels of 0 (control), 100, 300, 500 or 1000 mg/kg/day until experimental week 36. No statistically significant differences in incidences of preneoplastic lesions, papillomas, and carcinomas of the urinary bladder were evident in rats treated with 100-1000 mg/kg/day ETBE as compared with control values. Furthermore, the average numbers of preneoplastic or neoplastic lesions per unit length of basement membrane in rats given 100-1000 mg/kg/day ETBE were also comparable to control values. However, papillomatosis of the urinary bladder was found in 4 out of 30 rats (13%) in the group given 1000 mg/kg/day ETBE, and soft stones in the urinary bladder were found in 3 out of these 4 rats. The results thus demonstrated that ETBE did not exert promotional activity on urinary bladder carcinogenesis. However, papillomatosis of the urinary bladder developed in small numbers of the rats given ETBE at 1000 mg/kg/day but not in rats given 500 mg/kg/day or lower doses.

No MeSH data available.


Related in: MedlinePlus