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Colon preneoplastic lesions in animal models.

Suzui M, Morioka T, Yoshimi N - J Toxicol Pathol (2013)

Bottom Line: These lesions have been induced by commonly used chemical carcinogens such as azoxymethane (AOM), 1,2-dimethylhydrazine (DMH), methylnitrosourea (MUN), or 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP).Investigators can choose any procedures or methods to examine colonic preneoplastic lesions according to their interests and the objectives of their experiments.Based on topographical, histopathological, and biological features of colon cancer preneoplastic lesions in the animal model, we summarize and discuss the character and implications of these lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Toxicology, Graduate School of Medical Sciences and Medical School, Nagoya City University,1 Kawasumi, Mizuho-ku, Mizuho-cho, Nagoya 467-8601, Japan.

ABSTRACT
The animal model is a powerful and fundamental tool in the field of biochemical research including toxicology, carcinogenesis, cancer therapeutics and prevention. In the carcinogenesis animal model system, numerous examples of preneoplastic lesions have been isolated and investigated from various perspectives. This may indicate that several options of endpoints to evaluate carcinogenesis effect or therapeutic outcome are presently available; however, classification of preneoplastic lesions has become complicated. For instance, these lesions include aberrant crypt foci (ACF), dysplastic ACF, flat ACF, β-catenin accumulated crypts, and mucin-depleted foci. These lesions have been induced by commonly used chemical carcinogens such as azoxymethane (AOM), 1,2-dimethylhydrazine (DMH), methylnitrosourea (MUN), or 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Investigators can choose any procedures or methods to examine colonic preneoplastic lesions according to their interests and the objectives of their experiments. Based on topographical, histopathological, and biological features of colon cancer preneoplastic lesions in the animal model, we summarize and discuss the character and implications of these lesions.

No MeSH data available.


Related in: MedlinePlus

Topographic views of (A) ACF, (B) BCAC, and (C) MDF. (A) Note that three identical crypts are seen in one focus (methylene blue staining). (B) Crypts with accumulations of β-catenin protein in cytoplasm are present (immunohistochemical staining). (C) A focal lesion characterized by the absence or very small production of mucin (seen as very thin blue-stained crypts) is present (high-iron diamine-Alcian blue staining). ACF, aberrant crypt foci; BCAC, β-catenin accumulated crypts; MDF, mucin-depleted foci.
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fig_001: Topographic views of (A) ACF, (B) BCAC, and (C) MDF. (A) Note that three identical crypts are seen in one focus (methylene blue staining). (B) Crypts with accumulations of β-catenin protein in cytoplasm are present (immunohistochemical staining). (C) A focal lesion characterized by the absence or very small production of mucin (seen as very thin blue-stained crypts) is present (high-iron diamine-Alcian blue staining). ACF, aberrant crypt foci; BCAC, β-catenin accumulated crypts; MDF, mucin-depleted foci.

Mentions: Bird1 first reported in 1987 that when C57BL/6J mice were treated with azoxymethane (AOM), aberrant dysplastic crypts appeared in the colonic mucosa. After fixation with 10% buffered formalin and staining with methylene blue, these crypts were easily visualized in the topographic view of the colonic mucosa using a x4 objective (Fig. 1AFig. 1.


Colon preneoplastic lesions in animal models.

Suzui M, Morioka T, Yoshimi N - J Toxicol Pathol (2013)

Topographic views of (A) ACF, (B) BCAC, and (C) MDF. (A) Note that three identical crypts are seen in one focus (methylene blue staining). (B) Crypts with accumulations of β-catenin protein in cytoplasm are present (immunohistochemical staining). (C) A focal lesion characterized by the absence or very small production of mucin (seen as very thin blue-stained crypts) is present (high-iron diamine-Alcian blue staining). ACF, aberrant crypt foci; BCAC, β-catenin accumulated crypts; MDF, mucin-depleted foci.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921915&req=5

fig_001: Topographic views of (A) ACF, (B) BCAC, and (C) MDF. (A) Note that three identical crypts are seen in one focus (methylene blue staining). (B) Crypts with accumulations of β-catenin protein in cytoplasm are present (immunohistochemical staining). (C) A focal lesion characterized by the absence or very small production of mucin (seen as very thin blue-stained crypts) is present (high-iron diamine-Alcian blue staining). ACF, aberrant crypt foci; BCAC, β-catenin accumulated crypts; MDF, mucin-depleted foci.
Mentions: Bird1 first reported in 1987 that when C57BL/6J mice were treated with azoxymethane (AOM), aberrant dysplastic crypts appeared in the colonic mucosa. After fixation with 10% buffered formalin and staining with methylene blue, these crypts were easily visualized in the topographic view of the colonic mucosa using a x4 objective (Fig. 1AFig. 1.

Bottom Line: These lesions have been induced by commonly used chemical carcinogens such as azoxymethane (AOM), 1,2-dimethylhydrazine (DMH), methylnitrosourea (MUN), or 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP).Investigators can choose any procedures or methods to examine colonic preneoplastic lesions according to their interests and the objectives of their experiments.Based on topographical, histopathological, and biological features of colon cancer preneoplastic lesions in the animal model, we summarize and discuss the character and implications of these lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Toxicology, Graduate School of Medical Sciences and Medical School, Nagoya City University,1 Kawasumi, Mizuho-ku, Mizuho-cho, Nagoya 467-8601, Japan.

ABSTRACT
The animal model is a powerful and fundamental tool in the field of biochemical research including toxicology, carcinogenesis, cancer therapeutics and prevention. In the carcinogenesis animal model system, numerous examples of preneoplastic lesions have been isolated and investigated from various perspectives. This may indicate that several options of endpoints to evaluate carcinogenesis effect or therapeutic outcome are presently available; however, classification of preneoplastic lesions has become complicated. For instance, these lesions include aberrant crypt foci (ACF), dysplastic ACF, flat ACF, β-catenin accumulated crypts, and mucin-depleted foci. These lesions have been induced by commonly used chemical carcinogens such as azoxymethane (AOM), 1,2-dimethylhydrazine (DMH), methylnitrosourea (MUN), or 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). Investigators can choose any procedures or methods to examine colonic preneoplastic lesions according to their interests and the objectives of their experiments. Based on topographical, histopathological, and biological features of colon cancer preneoplastic lesions in the animal model, we summarize and discuss the character and implications of these lesions.

No MeSH data available.


Related in: MedlinePlus