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Methylene blue based device for pathogen reduction in human plasma.

Elikaei A, Hosseini SM, Sharifi Z, Latifi H, Nikbakht H, Mirshafiee H, Asadollahi A - Iran J Ped Hematol Oncol (2013)

Bottom Line: Two concentration of MB and 5 different illumination times were used.In 10 µm concentration of MB, HSV had 6.00±0.2 maximum log reduction that obtain after 60 minutes illumination and VSV had 5.50± 0.3 maximum log reduction after 75 minutes illumination.In 1 µM concentration of MB, HSV had 5.20±0.3 maximum log reduction that obtain after 60 minutes illumination and VSV had 4.90± 0.2 maximum log reduction after 75 minutes illumination.

View Article: PubMed Central - PubMed

Affiliation: Virology Laboratory, Research Centre of Iranian Blood Transfusion Organization (IBTO), Tehran, Iran.

ABSTRACT

Background: Despite improvement in safety of plasma transfusion some virus transmission still remains a problem. So as World Health Organization (WHO) recommends, many countries developed Pathogen Reduction Technologies (PRT) to inactivate pathogens, in plasma components. The Methylene Blue (MB) based methods is one of the most universal one. The purpose of this research was, produce a device that can inactivate viruses in MB environment.

Materials and methods: In this interventional study, each Plasma Sample was illuminated by 70Pieces (PCs) of 1 w red Light Emitting Diodes (LEDs) from one side. These LEDs emit light at central wavelength of 627 nm with 20 nm Full Width at Half Maximum (FWHM). Two model viruses Herpes Simplex Virus (HSV) and Vesicular Stomatitis Virus (VSV) were used and Tissue Culture 50% Infection Dose (TCID50) was used to calculate virus Log reduction. Two concentration of MB and 5 different illumination times were used.

Results: In 10 µm concentration of MB, HSV had 6.00±0.2 maximum log reduction that obtain after 60 minutes illumination and VSV had 5.50± 0.3 maximum log reduction after 75 minutes illumination. In 1 µM concentration of MB, HSV had 5.20±0.3 maximum log reduction that obtain after 60 minutes illumination and VSV had 4.90± 0.2 maximum log reduction after 75 minutes illumination.

Conclusion: Results of virus inactivation in this method were similar to other methods (P-value<0.05 in comparison with Spring method, and P-value>0.05 in comparison with Theraflex), and it showed this device could inactivate viruses according to WHO recommendation.

No MeSH data available.


Related in: MedlinePlus

Inactivation of HSV by 1 µM MB in combination with different light dose (depends on different illumination time), the most reduction obtained in 54 J/cm2 and increase in light dose decrease virus titration
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Figure 1: Inactivation of HSV by 1 µM MB in combination with different light dose (depends on different illumination time), the most reduction obtained in 54 J/cm2 and increase in light dose decrease virus titration

Mentions: In 1 µm concentration of MB, HSV had 5.20± 0.3 maximum log reductions that obtained in 54 J/cm2 after 60 minutes illumination (Figure 1), and VSV had 4.90± 0.2 maximum log reduction in 70 J/cm2 after 75 minutes illumination (Figure 2).


Methylene blue based device for pathogen reduction in human plasma.

Elikaei A, Hosseini SM, Sharifi Z, Latifi H, Nikbakht H, Mirshafiee H, Asadollahi A - Iran J Ped Hematol Oncol (2013)

Inactivation of HSV by 1 µM MB in combination with different light dose (depends on different illumination time), the most reduction obtained in 54 J/cm2 and increase in light dose decrease virus titration
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921873&req=5

Figure 1: Inactivation of HSV by 1 µM MB in combination with different light dose (depends on different illumination time), the most reduction obtained in 54 J/cm2 and increase in light dose decrease virus titration
Mentions: In 1 µm concentration of MB, HSV had 5.20± 0.3 maximum log reductions that obtained in 54 J/cm2 after 60 minutes illumination (Figure 1), and VSV had 4.90± 0.2 maximum log reduction in 70 J/cm2 after 75 minutes illumination (Figure 2).

Bottom Line: Two concentration of MB and 5 different illumination times were used.In 10 µm concentration of MB, HSV had 6.00±0.2 maximum log reduction that obtain after 60 minutes illumination and VSV had 5.50± 0.3 maximum log reduction after 75 minutes illumination.In 1 µM concentration of MB, HSV had 5.20±0.3 maximum log reduction that obtain after 60 minutes illumination and VSV had 4.90± 0.2 maximum log reduction after 75 minutes illumination.

View Article: PubMed Central - PubMed

Affiliation: Virology Laboratory, Research Centre of Iranian Blood Transfusion Organization (IBTO), Tehran, Iran.

ABSTRACT

Background: Despite improvement in safety of plasma transfusion some virus transmission still remains a problem. So as World Health Organization (WHO) recommends, many countries developed Pathogen Reduction Technologies (PRT) to inactivate pathogens, in plasma components. The Methylene Blue (MB) based methods is one of the most universal one. The purpose of this research was, produce a device that can inactivate viruses in MB environment.

Materials and methods: In this interventional study, each Plasma Sample was illuminated by 70Pieces (PCs) of 1 w red Light Emitting Diodes (LEDs) from one side. These LEDs emit light at central wavelength of 627 nm with 20 nm Full Width at Half Maximum (FWHM). Two model viruses Herpes Simplex Virus (HSV) and Vesicular Stomatitis Virus (VSV) were used and Tissue Culture 50% Infection Dose (TCID50) was used to calculate virus Log reduction. Two concentration of MB and 5 different illumination times were used.

Results: In 10 µm concentration of MB, HSV had 6.00±0.2 maximum log reduction that obtain after 60 minutes illumination and VSV had 5.50± 0.3 maximum log reduction after 75 minutes illumination. In 1 µM concentration of MB, HSV had 5.20±0.3 maximum log reduction that obtain after 60 minutes illumination and VSV had 4.90± 0.2 maximum log reduction after 75 minutes illumination.

Conclusion: Results of virus inactivation in this method were similar to other methods (P-value<0.05 in comparison with Spring method, and P-value>0.05 in comparison with Theraflex), and it showed this device could inactivate viruses according to WHO recommendation.

No MeSH data available.


Related in: MedlinePlus