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HIF-1, GLUT1, endoglin, and BIRC5 expression in urine samples obtained from patients with bladder malignancies - after photodynamic diagnosis (PDD).

Markowski M, Lipiński M, Krzeslak A, Forma E, Bryś M, Różański W - Cent European J Urol (2012)

Bottom Line: In the group after PDD, a statistically significant overexpression of HIF-1 was observed.The differences between the group with PDD and the group without it can be connected with the direct influence of PDD on malignant tissue, which can cause overexpression of HIF-1 only.This is, however, only a hypothesis and needs further study.

View Article: PubMed Central - PubMed

Affiliation: Clinic of Urology and Urological Oncology, Medical University of Łódź, Copernicus Memorial Hospital, Łódź, Poland.

ABSTRACT

Introduction: Bladder cancer (BC) is a serious medical problem. The high rate of recurrence and progression demands the development of new methods, such as genetic markers, which allow diagnosis and patient follow-up.

Objectives: The aim of this study was to compare expression of HIF-1, GLUT1, endoglin, and BRIC5 in patients without and those with BC. The second group was divided into sub-groups: those without a history of PDD (photodynamic diagnosis) in the diagnostic process and those after PDD.

Methods: Patients with BC were diagnosed using the PDD method using hexaminolevulinate (Hexvix(®)). The expressions of HIF-1, GLUT1, endoglin, and BRIC5 genes were established in urine specimens by real-time quantitative polymerase chain reaction (PCR).

Results: The expressions of all tested genes were higher in the group of patients with BC than in the group without BC. In the group after PDD, a statistically significant overexpression of HIF-1 was observed. In this group, changes were not observed in cases of the other three tested genes.

Conclusions: The differences between the group with PDD and the group without it can be connected with the direct influence of PDD on malignant tissue, which can cause overexpression of HIF-1 only. This is, however, only a hypothesis and needs further study.

No MeSH data available.


Related in: MedlinePlus

HIF-1α mean mRNA expression level in bladder carcinomas; a comparison between subgroups with different tumor grade
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Figure 0002: HIF-1α mean mRNA expression level in bladder carcinomas; a comparison between subgroups with different tumor grade

Mentions: In the set after PDD (16/32), the HIF-1 mRNA expression was on a level of 842.29 ±252.15 (Figs. 1, 2).


HIF-1, GLUT1, endoglin, and BIRC5 expression in urine samples obtained from patients with bladder malignancies - after photodynamic diagnosis (PDD).

Markowski M, Lipiński M, Krzeslak A, Forma E, Bryś M, Różański W - Cent European J Urol (2012)

HIF-1α mean mRNA expression level in bladder carcinomas; a comparison between subgroups with different tumor grade
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921788&req=5

Figure 0002: HIF-1α mean mRNA expression level in bladder carcinomas; a comparison between subgroups with different tumor grade
Mentions: In the set after PDD (16/32), the HIF-1 mRNA expression was on a level of 842.29 ±252.15 (Figs. 1, 2).

Bottom Line: In the group after PDD, a statistically significant overexpression of HIF-1 was observed.The differences between the group with PDD and the group without it can be connected with the direct influence of PDD on malignant tissue, which can cause overexpression of HIF-1 only.This is, however, only a hypothesis and needs further study.

View Article: PubMed Central - PubMed

Affiliation: Clinic of Urology and Urological Oncology, Medical University of Łódź, Copernicus Memorial Hospital, Łódź, Poland.

ABSTRACT

Introduction: Bladder cancer (BC) is a serious medical problem. The high rate of recurrence and progression demands the development of new methods, such as genetic markers, which allow diagnosis and patient follow-up.

Objectives: The aim of this study was to compare expression of HIF-1, GLUT1, endoglin, and BRIC5 in patients without and those with BC. The second group was divided into sub-groups: those without a history of PDD (photodynamic diagnosis) in the diagnostic process and those after PDD.

Methods: Patients with BC were diagnosed using the PDD method using hexaminolevulinate (Hexvix(®)). The expressions of HIF-1, GLUT1, endoglin, and BRIC5 genes were established in urine specimens by real-time quantitative polymerase chain reaction (PCR).

Results: The expressions of all tested genes were higher in the group of patients with BC than in the group without BC. In the group after PDD, a statistically significant overexpression of HIF-1 was observed. In this group, changes were not observed in cases of the other three tested genes.

Conclusions: The differences between the group with PDD and the group without it can be connected with the direct influence of PDD on malignant tissue, which can cause overexpression of HIF-1 only. This is, however, only a hypothesis and needs further study.

No MeSH data available.


Related in: MedlinePlus