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Polymorphic variants of H-RAS protooncogene and their possible role in bladder cancer etiology.

Traczyk M, Borkowska E, Rożniecki M, Purpurowicz R, Jędrzejczyk A, Marks P, Pietrusiński M, Jabłonowski Z, Sosnowski M, Kałużewski B - Cent European J Urol (2012)

Bottom Line: In order to assess the clinical relevance of the polymorphism, the results were compared with those for the control group.DNA polymorphisms start to play an important role in evaluation of disease risk and progression.The occurrence of multiple variants of the same gene may contribute to differences in reactions to specific medications and sensitivity to carcinogens or DNA repair capacity.

View Article: PubMed Central - PubMed

Affiliation: Chair of Clinical and Laboratory Genetics, Medical University of Łódź, Poland.

ABSTRACT

Introduction: H-RAS gene is a protooncogene encoding p21ras, a small protein with GTPase activity. This protein is a component of many signaling cascades, while mutations in H-RAS gene are often found in urinary bladder cancer and leads to continuous transmission of signals stimulating cancer cell growth and proliferation. The T81C polymorphism of H-RAS gene is a SNP, which, although does not seem to impair p21ras protein structure and function, may contribute to the development of bladder cancer.

Objectives: The aim of our study was to characterize the prevalence and clinical significance of T81C polymorphism in patients with diagnosed bladder cancer.

Materials and methods: 132 patients with diagnosed urinary bladder cancer were included in this study. The control group consisted of 106 healthy individuals. The experimental material was DNA, isolated from tumor tissue and peripheral blood lymphocytes. T81C polymorphism was detected using the MSSCP method and DNA sequencing.

Results: In the examined DNA samples, frequent polymorphic variations were found in codon 27 of H-RAS gene. In order to assess the clinical relevance of the polymorphism, the results were compared with those for the control group. The homozygous CC variant occurred more frequently in bladder cancer patients than in healthy individuals.

Conclusions: DNA polymorphisms start to play an important role in evaluation of disease risk and progression. The occurrence of multiple variants of the same gene may contribute to differences in reactions to specific medications and sensitivity to carcinogens or DNA repair capacity. Our study demonstrated T81C polymorphism of H-RAS gene to have seemingly been associated with an increased risk of bladder cancer development.

No MeSH data available.


Related in: MedlinePlus

Structure of H-RAS gene. Black figures depict encoding exons.
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Figure 0001: Structure of H-RAS gene. Black figures depict encoding exons.

Mentions: The H-RAS (Harvey rat sarcoma viral oncogene homologue) protooncogene is located at the terminal part of the short arm of chromosome 11. It was discovered in 1980 as an oncogene of sarcoma viruses [3]. It consists of four encoding and one noncoding exon, the latter localized closer to the 5’ end (Fig. 1). A point mutation in one of the three hot spots (codons 12, 13, and 61) may result in continuous stimulation of proliferation and development of many types of cancer [4, 5].


Polymorphic variants of H-RAS protooncogene and their possible role in bladder cancer etiology.

Traczyk M, Borkowska E, Rożniecki M, Purpurowicz R, Jędrzejczyk A, Marks P, Pietrusiński M, Jabłonowski Z, Sosnowski M, Kałużewski B - Cent European J Urol (2012)

Structure of H-RAS gene. Black figures depict encoding exons.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921776&req=5

Figure 0001: Structure of H-RAS gene. Black figures depict encoding exons.
Mentions: The H-RAS (Harvey rat sarcoma viral oncogene homologue) protooncogene is located at the terminal part of the short arm of chromosome 11. It was discovered in 1980 as an oncogene of sarcoma viruses [3]. It consists of four encoding and one noncoding exon, the latter localized closer to the 5’ end (Fig. 1). A point mutation in one of the three hot spots (codons 12, 13, and 61) may result in continuous stimulation of proliferation and development of many types of cancer [4, 5].

Bottom Line: In order to assess the clinical relevance of the polymorphism, the results were compared with those for the control group.DNA polymorphisms start to play an important role in evaluation of disease risk and progression.The occurrence of multiple variants of the same gene may contribute to differences in reactions to specific medications and sensitivity to carcinogens or DNA repair capacity.

View Article: PubMed Central - PubMed

Affiliation: Chair of Clinical and Laboratory Genetics, Medical University of Łódź, Poland.

ABSTRACT

Introduction: H-RAS gene is a protooncogene encoding p21ras, a small protein with GTPase activity. This protein is a component of many signaling cascades, while mutations in H-RAS gene are often found in urinary bladder cancer and leads to continuous transmission of signals stimulating cancer cell growth and proliferation. The T81C polymorphism of H-RAS gene is a SNP, which, although does not seem to impair p21ras protein structure and function, may contribute to the development of bladder cancer.

Objectives: The aim of our study was to characterize the prevalence and clinical significance of T81C polymorphism in patients with diagnosed bladder cancer.

Materials and methods: 132 patients with diagnosed urinary bladder cancer were included in this study. The control group consisted of 106 healthy individuals. The experimental material was DNA, isolated from tumor tissue and peripheral blood lymphocytes. T81C polymorphism was detected using the MSSCP method and DNA sequencing.

Results: In the examined DNA samples, frequent polymorphic variations were found in codon 27 of H-RAS gene. In order to assess the clinical relevance of the polymorphism, the results were compared with those for the control group. The homozygous CC variant occurred more frequently in bladder cancer patients than in healthy individuals.

Conclusions: DNA polymorphisms start to play an important role in evaluation of disease risk and progression. The occurrence of multiple variants of the same gene may contribute to differences in reactions to specific medications and sensitivity to carcinogens or DNA repair capacity. Our study demonstrated T81C polymorphism of H-RAS gene to have seemingly been associated with an increased risk of bladder cancer development.

No MeSH data available.


Related in: MedlinePlus