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Immunohistochemical detection of angiotensin AT 1 and AT 2 receptors in prostate cancer.

Pawlikowski M, Minias R, Sosnowski M, Zieliński KW - Cent European J Urol (2011)

Bottom Line: For visualization of primary antibodies, the streptavidin-biotin-peroxidase technique was applied.The positive immunostaining with both anti- AT1 and anti-AT2 antibodies can be found in stromal as well as epithelial structures.The results of quantitative evaluation showed the positive correlation between AT1 and AT2 expressions in neoplastic epithelium and overexpression of both AT1 and AT2 in neoplastic epithelium of Gleason grade 2, but not in cancerous structures of Gleason grades 3-5.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroendocrinology, Chair of Endocrinology, Medical University of Łódź, Poland.

ABSTRACT

Introduction: The human prostate gland contains all the compounds of the renin-angiotensin system (RAS), including AT1 and AT2 angiotensin receptors. The role of local RAS in the prostate pathology is recently discussed. The aim of the present study was the evaluation of AT1 and AT2 expressions in human prostate cancers.

Material and methods: The investigation was performed in 20 paraffin-embedded needle biopsy specimens from routine diagnostic prostate cancer biopsies. The specimens were stained with hematoxylin and eosin and immunostained with anti-AT1 and anti-AT2 antibodies. For visualization of primary antibodies, the streptavidin-biotin-peroxidase technique was applied. The expression of both receptor proteins was evaluated quantitatively using image analysis method.

Results: The positive immunostaining with both anti- AT1 and anti-AT2 antibodies can be found in stromal as well as epithelial structures. The results of quantitative evaluation showed the positive correlation between AT1 and AT2 expressions in neoplastic epithelium and overexpression of both AT1 and AT2 in neoplastic epithelium of Gleason grade 2, but not in cancerous structures of Gleason grades 3-5.

Conclusions: The data on AT1 and AT2 receptor expressions may suggest the involvement of RAS in prostate cancerogenesis. Moreover, the persistence of AT1 receptors in prostate cancer speaks in favor of attempts to use of AT1 receptor blockers (i.e. sartanes) and/or AT2 agonists in prostate cancer prophylaxis and/or treatment.

No MeSH data available.


Related in: MedlinePlus

An assessment image of a Gleason 3 carcinomatous infiltration (H&E, magn. 50x).
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Figure 0001: An assessment image of a Gleason 3 carcinomatous infiltration (H&E, magn. 50x).

Mentions: The statistic analysis was performed using Statistica ver. 5.0 software. After the F-Snedecor test for variance equality, the appropriate double-sided t-Student test was used for estimating the statistic significance of differences of densitometric values of various types of glands (p <0.05). Moreover, the Pearson correlation coefficient r was calculated. The correlation between densitometric values of glands in the same areas stained with AT1 and AT2 antibodies was analyzed. The steps of the image analysis are presented below (Figs. 1–3).


Immunohistochemical detection of angiotensin AT 1 and AT 2 receptors in prostate cancer.

Pawlikowski M, Minias R, Sosnowski M, Zieliński KW - Cent European J Urol (2011)

An assessment image of a Gleason 3 carcinomatous infiltration (H&E, magn. 50x).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921746&req=5

Figure 0001: An assessment image of a Gleason 3 carcinomatous infiltration (H&E, magn. 50x).
Mentions: The statistic analysis was performed using Statistica ver. 5.0 software. After the F-Snedecor test for variance equality, the appropriate double-sided t-Student test was used for estimating the statistic significance of differences of densitometric values of various types of glands (p <0.05). Moreover, the Pearson correlation coefficient r was calculated. The correlation between densitometric values of glands in the same areas stained with AT1 and AT2 antibodies was analyzed. The steps of the image analysis are presented below (Figs. 1–3).

Bottom Line: For visualization of primary antibodies, the streptavidin-biotin-peroxidase technique was applied.The positive immunostaining with both anti- AT1 and anti-AT2 antibodies can be found in stromal as well as epithelial structures.The results of quantitative evaluation showed the positive correlation between AT1 and AT2 expressions in neoplastic epithelium and overexpression of both AT1 and AT2 in neoplastic epithelium of Gleason grade 2, but not in cancerous structures of Gleason grades 3-5.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroendocrinology, Chair of Endocrinology, Medical University of Łódź, Poland.

ABSTRACT

Introduction: The human prostate gland contains all the compounds of the renin-angiotensin system (RAS), including AT1 and AT2 angiotensin receptors. The role of local RAS in the prostate pathology is recently discussed. The aim of the present study was the evaluation of AT1 and AT2 expressions in human prostate cancers.

Material and methods: The investigation was performed in 20 paraffin-embedded needle biopsy specimens from routine diagnostic prostate cancer biopsies. The specimens were stained with hematoxylin and eosin and immunostained with anti-AT1 and anti-AT2 antibodies. For visualization of primary antibodies, the streptavidin-biotin-peroxidase technique was applied. The expression of both receptor proteins was evaluated quantitatively using image analysis method.

Results: The positive immunostaining with both anti- AT1 and anti-AT2 antibodies can be found in stromal as well as epithelial structures. The results of quantitative evaluation showed the positive correlation between AT1 and AT2 expressions in neoplastic epithelium and overexpression of both AT1 and AT2 in neoplastic epithelium of Gleason grade 2, but not in cancerous structures of Gleason grades 3-5.

Conclusions: The data on AT1 and AT2 receptor expressions may suggest the involvement of RAS in prostate cancerogenesis. Moreover, the persistence of AT1 receptors in prostate cancer speaks in favor of attempts to use of AT1 receptor blockers (i.e. sartanes) and/or AT2 agonists in prostate cancer prophylaxis and/or treatment.

No MeSH data available.


Related in: MedlinePlus