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Effect of ALA-mediated photodynamic therapy in combination with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on bladder cancer cells.

Szliszka E, Kawczyk-Krupka A, Czuba ZP, Sieron A, Krol W - Cent European J Urol (2011)

Bottom Line: Our study confirmed that T24 and 647V bladder cancer cells were resistant to TRAIL, whereas SW780 cells were sensitive to TRAIL.We showed for the first time that pretreatment with low dose of PDT significantly sensitizes bladder cancer cells to TRAIL induced cytotoxicity.The obtained results suggest that combined treatment of TRAIL and PDT may provide the basis for a new strategy to induce cytotoxicity in bladder cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Chair and Department of Microbiology and Immunology in Zabrze, Medical University of Silesia in Katowice, Poland.

ABSTRACT

Introduction: Photodynamic therapy (PDT), an alternative treatment modality for superficial bladder tumors is based on the interaction of a photosensitizer with light. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising candidate for anticancer therapy due to its ability to selectively induce apoptosis in cancer cells. However, not all tumor cells are sensitive to TRAIL. TRAIL-resistant cancer cells can be sensitized to TRAIL induced apoptosis by anticancer agents.

Objective: We investigated the combined cytotoxic effect of TRAIL and PDT with 5-aminolevulinic acid (ALA) on bladder cancer cells.

Materials and methods: THREE HUMAN BLADDER TRANSITIONAL CANCER CELL LINES: T24, 647V, and SW780 were treated with TRAIL and/or ALA-mediated PDT. Cytotoxicity was determined by MTT and LDH assay.

Results: Our study confirmed that T24 and 647V bladder cancer cells were resistant to TRAIL, whereas SW780 cells were sensitive to TRAIL. We therefore examined the cytotoxic effect of TRAIL in combination with ALA-mediated PDT on bladder cancer cells. We showed for the first time that pretreatment with low dose of PDT significantly sensitizes bladder cancer cells to TRAIL induced cytotoxicity.

Conclusion: ALA-mediated PDT augments the cytotoxic effect of TRAIL on transitional cancer cells of bladder. The obtained results suggest that combined treatment of TRAIL and PDT may provide the basis for a new strategy to induce cytotoxicity in bladder cancer cells.

No MeSH data available.


Related in: MedlinePlus

Cytotoxic effect of TRAIL on SW780 bladder cancer cells pretreated with ALA-mediated PDT.
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Figure 0004: Cytotoxic effect of TRAIL on SW780 bladder cancer cells pretreated with ALA-mediated PDT.

Mentions: The bladder cancer cells were treated with low dose of PDT (ALA at concentration of 25 μM and VIS: 400-750 nm, 7.5 J/cm2) and 3 hours after irradiation subsequent incubation with TRAIL at concentration of 5-50 ng/ml for 18-hours was done. The cytotoxicity of TRAIL in combination with ALA-mediated PDT against bladder cancer cell lines is shown in Figures 4-6. PDT significantly augmented TRAIL-induced cell death in all bladder cancer lines. In TRAIL-resistant cancer cells, 647V and T24, PDT increased cytotoxic effect of TRAIL and sensitized both TCC lines to TRAIL-induced cell death. The cytotoxicity of combined treatment TRAIL at the concentrations of 50 ng/ml with PDT against bladder cancer cells was as follows: 98.9 ±0.6% for SW780, 67.2 ±4.9% for 647V, 45.5 ±1.5% for T24 cells. The photoactivated ALA also enhanced the cytotoxic effect of lower concentrations of TRAIL (5 ng/ml and 10 ng/ml) in TCC cells by 18-37% compared to PDT or TRAIL alone.


Effect of ALA-mediated photodynamic therapy in combination with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on bladder cancer cells.

Szliszka E, Kawczyk-Krupka A, Czuba ZP, Sieron A, Krol W - Cent European J Urol (2011)

Cytotoxic effect of TRAIL on SW780 bladder cancer cells pretreated with ALA-mediated PDT.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921731&req=5

Figure 0004: Cytotoxic effect of TRAIL on SW780 bladder cancer cells pretreated with ALA-mediated PDT.
Mentions: The bladder cancer cells were treated with low dose of PDT (ALA at concentration of 25 μM and VIS: 400-750 nm, 7.5 J/cm2) and 3 hours after irradiation subsequent incubation with TRAIL at concentration of 5-50 ng/ml for 18-hours was done. The cytotoxicity of TRAIL in combination with ALA-mediated PDT against bladder cancer cell lines is shown in Figures 4-6. PDT significantly augmented TRAIL-induced cell death in all bladder cancer lines. In TRAIL-resistant cancer cells, 647V and T24, PDT increased cytotoxic effect of TRAIL and sensitized both TCC lines to TRAIL-induced cell death. The cytotoxicity of combined treatment TRAIL at the concentrations of 50 ng/ml with PDT against bladder cancer cells was as follows: 98.9 ±0.6% for SW780, 67.2 ±4.9% for 647V, 45.5 ±1.5% for T24 cells. The photoactivated ALA also enhanced the cytotoxic effect of lower concentrations of TRAIL (5 ng/ml and 10 ng/ml) in TCC cells by 18-37% compared to PDT or TRAIL alone.

Bottom Line: Our study confirmed that T24 and 647V bladder cancer cells were resistant to TRAIL, whereas SW780 cells were sensitive to TRAIL.We showed for the first time that pretreatment with low dose of PDT significantly sensitizes bladder cancer cells to TRAIL induced cytotoxicity.The obtained results suggest that combined treatment of TRAIL and PDT may provide the basis for a new strategy to induce cytotoxicity in bladder cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Chair and Department of Microbiology and Immunology in Zabrze, Medical University of Silesia in Katowice, Poland.

ABSTRACT

Introduction: Photodynamic therapy (PDT), an alternative treatment modality for superficial bladder tumors is based on the interaction of a photosensitizer with light. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising candidate for anticancer therapy due to its ability to selectively induce apoptosis in cancer cells. However, not all tumor cells are sensitive to TRAIL. TRAIL-resistant cancer cells can be sensitized to TRAIL induced apoptosis by anticancer agents.

Objective: We investigated the combined cytotoxic effect of TRAIL and PDT with 5-aminolevulinic acid (ALA) on bladder cancer cells.

Materials and methods: THREE HUMAN BLADDER TRANSITIONAL CANCER CELL LINES: T24, 647V, and SW780 were treated with TRAIL and/or ALA-mediated PDT. Cytotoxicity was determined by MTT and LDH assay.

Results: Our study confirmed that T24 and 647V bladder cancer cells were resistant to TRAIL, whereas SW780 cells were sensitive to TRAIL. We therefore examined the cytotoxic effect of TRAIL in combination with ALA-mediated PDT on bladder cancer cells. We showed for the first time that pretreatment with low dose of PDT significantly sensitizes bladder cancer cells to TRAIL induced cytotoxicity.

Conclusion: ALA-mediated PDT augments the cytotoxic effect of TRAIL on transitional cancer cells of bladder. The obtained results suggest that combined treatment of TRAIL and PDT may provide the basis for a new strategy to induce cytotoxicity in bladder cancer cells.

No MeSH data available.


Related in: MedlinePlus