Limits...
Schwann cells - a new hope in tissue engineered urinary bladder innervation. A method of cell isolation.

Adamowicz J, Drewa T, Tworkiewicz J, Kloskowski T, Nowacki M, Pokrywczyńska M - Cent European J Urol (2011)

Bottom Line: Colonies of cells that morphologically resembled Schwann cells were visible by light microscopy on the second day of in vitro cultivation.Homogeneity of the primary cultures increased in the last day of cultivation to 60%.Schwann cells isolation from predegenerated peripheral nerve is effective and can delivered require amount of cells for transplantation to urinary bladder graft.

View Article: PubMed Central - PubMed

Affiliation: Tissue Engineering Department, University of Nicolaus Copernicus, Bydgoszcz, Poland.

ABSTRACT

Introduction: There are not any effective method to induce the innervation of urinary bladder wall graft after augmentation. Neurons from urinary bladder wall and omentium can not elongate and branch in graft because of lack of neurotrophic factors. The best source of these neurotrophic factors are Schwann cells which can be transplanted into urinary bladder wall graft. To transplant Schwann cells the proper amount of cells is needed which can be only obtain during in vitro Schwann cell cultivation. We introduce the results of Schwann cell isolation and in vitro cultivation.

Materials and methods: 33 Wistar rats, males (350 gr.) were used in this study. Animal were divited into two groups (n = 15). Cell cultures were established in both groups on 5, 6, 7, 8 nad 9 day after nerve injury. In first group the digestion time with colagenase and trypsyne was 2.5 h and in second one 3.5 h.

Results: A larger number of cells were isolated from the degenerated sciatic nerve. Colonies of cells that morphologically resembled Schwann cells were visible by light microscopy on the second day of in vitro cultivation. Homogeneity of the primary cultures increased in the last day of cultivation to 60%.

Conclusions: Schwann cells isolation from predegenerated peripheral nerve is effective and can delivered require amount of cells for transplantation to urinary bladder graft.

No MeSH data available.


Related in: MedlinePlus

Homogeneity of Schwann cell primary culture on third and last day of cultivation. Cultures were established from nerves taken after different times from injury. Digestion time was 3.5 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3921712&req=5

Figure 0003: Homogeneity of Schwann cell primary culture on third and last day of cultivation. Cultures were established from nerves taken after different times from injury. Digestion time was 3.5 h.

Mentions: Homogeneity of the primary cultures increased in the last day of cultivation to 60% (Figs. 2–3). On the 7th day they reached around 60%. Homogeneity of culture did not depend on the peripheral nerve degeneration. Cultures starting on 5th day from nerve injury were characterized by a smaller number of Schwann cells. In this case, on the 3rd day the homogeneity of cultures was about 33% and increased on the 7th day to about 40%. The digestion time did not affect the homogeneity of the primary culture (P = 0.5). Marked cells with the marker S-100 showed the presence of Schwann cells, which formed oval connecting colonies (Fig. 4).


Schwann cells - a new hope in tissue engineered urinary bladder innervation. A method of cell isolation.

Adamowicz J, Drewa T, Tworkiewicz J, Kloskowski T, Nowacki M, Pokrywczyńska M - Cent European J Urol (2011)

Homogeneity of Schwann cell primary culture on third and last day of cultivation. Cultures were established from nerves taken after different times from injury. Digestion time was 3.5 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921712&req=5

Figure 0003: Homogeneity of Schwann cell primary culture on third and last day of cultivation. Cultures were established from nerves taken after different times from injury. Digestion time was 3.5 h.
Mentions: Homogeneity of the primary cultures increased in the last day of cultivation to 60% (Figs. 2–3). On the 7th day they reached around 60%. Homogeneity of culture did not depend on the peripheral nerve degeneration. Cultures starting on 5th day from nerve injury were characterized by a smaller number of Schwann cells. In this case, on the 3rd day the homogeneity of cultures was about 33% and increased on the 7th day to about 40%. The digestion time did not affect the homogeneity of the primary culture (P = 0.5). Marked cells with the marker S-100 showed the presence of Schwann cells, which formed oval connecting colonies (Fig. 4).

Bottom Line: Colonies of cells that morphologically resembled Schwann cells were visible by light microscopy on the second day of in vitro cultivation.Homogeneity of the primary cultures increased in the last day of cultivation to 60%.Schwann cells isolation from predegenerated peripheral nerve is effective and can delivered require amount of cells for transplantation to urinary bladder graft.

View Article: PubMed Central - PubMed

Affiliation: Tissue Engineering Department, University of Nicolaus Copernicus, Bydgoszcz, Poland.

ABSTRACT

Introduction: There are not any effective method to induce the innervation of urinary bladder wall graft after augmentation. Neurons from urinary bladder wall and omentium can not elongate and branch in graft because of lack of neurotrophic factors. The best source of these neurotrophic factors are Schwann cells which can be transplanted into urinary bladder wall graft. To transplant Schwann cells the proper amount of cells is needed which can be only obtain during in vitro Schwann cell cultivation. We introduce the results of Schwann cell isolation and in vitro cultivation.

Materials and methods: 33 Wistar rats, males (350 gr.) were used in this study. Animal were divited into two groups (n = 15). Cell cultures were established in both groups on 5, 6, 7, 8 nad 9 day after nerve injury. In first group the digestion time with colagenase and trypsyne was 2.5 h and in second one 3.5 h.

Results: A larger number of cells were isolated from the degenerated sciatic nerve. Colonies of cells that morphologically resembled Schwann cells were visible by light microscopy on the second day of in vitro cultivation. Homogeneity of the primary cultures increased in the last day of cultivation to 60%.

Conclusions: Schwann cells isolation from predegenerated peripheral nerve is effective and can delivered require amount of cells for transplantation to urinary bladder graft.

No MeSH data available.


Related in: MedlinePlus