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Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study.

Shirazi M, Noorafshan A, Farrokhi A - Cent European J Urol (2011)

Bottom Line: The experimental group received pentoxifylline 100 mg/kg per day via oral gavages for 4-weeks.The control group received the same dose of normal saline.Pentoxifylline reduces interstitial renal fibrosis after partial urethral obstruction in rats.

View Article: PubMed Central - PubMed

Affiliation: Histomorphometry & Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objective: Chronic renal failure due to tubulointerstitial fibrosis is one of complications of lower urinary tract obstruction. Since pentoxifylline is a xanthine derivative that inhibits some inflammatory mediators, we conducted this study to investigate whether pentoxifylline inhibits renal fibrosis in a rat model of partial urethral obstruction (PUO).

Methods: All the rats underwent experimental PUO. Then, the animals were divided randomly into two groups: positive control group and experimental group. The experimental group received pentoxifylline 100 mg/kg per day via oral gavages for 4-weeks. The control group received the same dose of normal saline. After 4-weeks, all the rats underwent left nephrectomy. Kidney volume and weight and fractional and absolute volumes of the glomeruli, tubules, interstitium and vessels were determined with stereological methods. To reduce the workload of reference (kidney) volume estimation, the total kidney volume was determined after estimation of tissue shrinkage on isotropic uniform random histological sections. The total volume (amount) of each renal structure including fibrosis was estimated to avoid the bias conclusion due to relying on volume density alone.

Results: The absolute volume of interstitial fibrosis was lower in the experimental group (PUO with pentoxifylline treatment) (~84%; p ≤0.006) in comparison with the control group (PUO with no treatment).

Conclusion: Pentoxifylline reduces interstitial renal fibrosis after partial urethral obstruction in rats.

No MeSH data available.


Related in: MedlinePlus

Dot plot of the absolute volume (mm3) of the glomeruli (Glom), proximal convoluted tubules (PCT), distal convoluted tubules (DCT), collecting ducts (CD), loop of Henle (LH), interstitial fibrosis (IS), and vessels in the positive control (PUO) (first 7 parameters) and PUO + pentoxifylline (second 7 parameters) groups.
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Figure 0003: Dot plot of the absolute volume (mm3) of the glomeruli (Glom), proximal convoluted tubules (PCT), distal convoluted tubules (DCT), collecting ducts (CD), loop of Henle (LH), interstitial fibrosis (IS), and vessels in the positive control (PUO) (first 7 parameters) and PUO + pentoxifylline (second 7 parameters) groups.

Mentions: The details of kidney weight, kidney volume, and absolute volume of the cortex and medulla are shown in Table 1. Kidney weight and volume and absolute volume of the cortex and medulla did not show any significant differences between PUO and PUO plus pentoxifylline. As seen in Table 2 and Figure 3 (plot) the mean absolute volume of the glomeruli and tubules did not show significant differences between two groups. The mean absolute volume of interstitial tissue in the pentoxifylline-treated group was ∼84% less than that of the control group (p <0.006). Also an increase of the mean absolute of the vessels in the pentoxifylline group in comparison with control group was seen, but it was not significant statistically.


Effects of pentoxifylline on renal structure after urethral obstruction in rat: A stereological study.

Shirazi M, Noorafshan A, Farrokhi A - Cent European J Urol (2011)

Dot plot of the absolute volume (mm3) of the glomeruli (Glom), proximal convoluted tubules (PCT), distal convoluted tubules (DCT), collecting ducts (CD), loop of Henle (LH), interstitial fibrosis (IS), and vessels in the positive control (PUO) (first 7 parameters) and PUO + pentoxifylline (second 7 parameters) groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921697&req=5

Figure 0003: Dot plot of the absolute volume (mm3) of the glomeruli (Glom), proximal convoluted tubules (PCT), distal convoluted tubules (DCT), collecting ducts (CD), loop of Henle (LH), interstitial fibrosis (IS), and vessels in the positive control (PUO) (first 7 parameters) and PUO + pentoxifylline (second 7 parameters) groups.
Mentions: The details of kidney weight, kidney volume, and absolute volume of the cortex and medulla are shown in Table 1. Kidney weight and volume and absolute volume of the cortex and medulla did not show any significant differences between PUO and PUO plus pentoxifylline. As seen in Table 2 and Figure 3 (plot) the mean absolute volume of the glomeruli and tubules did not show significant differences between two groups. The mean absolute volume of interstitial tissue in the pentoxifylline-treated group was ∼84% less than that of the control group (p <0.006). Also an increase of the mean absolute of the vessels in the pentoxifylline group in comparison with control group was seen, but it was not significant statistically.

Bottom Line: The experimental group received pentoxifylline 100 mg/kg per day via oral gavages for 4-weeks.The control group received the same dose of normal saline.Pentoxifylline reduces interstitial renal fibrosis after partial urethral obstruction in rats.

View Article: PubMed Central - PubMed

Affiliation: Histomorphometry & Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT

Objective: Chronic renal failure due to tubulointerstitial fibrosis is one of complications of lower urinary tract obstruction. Since pentoxifylline is a xanthine derivative that inhibits some inflammatory mediators, we conducted this study to investigate whether pentoxifylline inhibits renal fibrosis in a rat model of partial urethral obstruction (PUO).

Methods: All the rats underwent experimental PUO. Then, the animals were divided randomly into two groups: positive control group and experimental group. The experimental group received pentoxifylline 100 mg/kg per day via oral gavages for 4-weeks. The control group received the same dose of normal saline. After 4-weeks, all the rats underwent left nephrectomy. Kidney volume and weight and fractional and absolute volumes of the glomeruli, tubules, interstitium and vessels were determined with stereological methods. To reduce the workload of reference (kidney) volume estimation, the total kidney volume was determined after estimation of tissue shrinkage on isotropic uniform random histological sections. The total volume (amount) of each renal structure including fibrosis was estimated to avoid the bias conclusion due to relying on volume density alone.

Results: The absolute volume of interstitial fibrosis was lower in the experimental group (PUO with pentoxifylline treatment) (~84%; p ≤0.006) in comparison with the control group (PUO with no treatment).

Conclusion: Pentoxifylline reduces interstitial renal fibrosis after partial urethral obstruction in rats.

No MeSH data available.


Related in: MedlinePlus