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Anti-stokes fluorescent probe with incoherent excitation.

Li Y, Zhou S, Dong G, Peng M, Wondraczek L, Qiu J - Sci Rep (2014)

Bottom Line: Although inorganic anti-Stokes fluorescent probes have long been developed, the operational mode of today's most advanced examples still involves the harsh requirement of coherent laser excitation, which often yields unexpected light disturbance or even photon-induced deterioration during optical imaging.We show that the probe can be operated under light-emitting diode excitation and provides tunable anti-Stokes energy shift and decay kinetics, which allow for rapid and deep tissue imaging over a very large area with negligible photodestruction.Charging of the probe can be achieved by either X-rays or ultraviolet-visible light irradiation, which enables multiplexed detection and function integration with standard X-ray medical imaging devices.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Luminescent Materials and Devices, Institute of Optical Communication Materials, School of Materials Science and Technology, South China University of Technology, Guangzhou 510640, China.

ABSTRACT
Although inorganic anti-Stokes fluorescent probes have long been developed, the operational mode of today's most advanced examples still involves the harsh requirement of coherent laser excitation, which often yields unexpected light disturbance or even photon-induced deterioration during optical imaging. Here, we demonstrate an efficient anti-Stokes fluorescent probe with incoherent excitation. We show that the probe can be operated under light-emitting diode excitation and provides tunable anti-Stokes energy shift and decay kinetics, which allow for rapid and deep tissue imaging over a very large area with negligible photodestruction. Charging of the probe can be achieved by either X-rays or ultraviolet-visible light irradiation, which enables multiplexed detection and function integration with standard X-ray medical imaging devices.

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Related in: MedlinePlus

Anti-Stokes luminescence stability and reproducibility.(a) Evolution of luminescence monitored at 700 nm under continuous illumination with an incoherent LED (7.87 mW/cm2) operating at a wavelength of 800 nm. (b) Reproducibility of anti-Stokes luminescence generation during 17 on/off cycles over a period of 10000 s. Before measurements, effective optical charging of the probe can be done by UV-vis light for 5 min, the measurements were taken at the interval of 24 hours after charging.
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f4: Anti-Stokes luminescence stability and reproducibility.(a) Evolution of luminescence monitored at 700 nm under continuous illumination with an incoherent LED (7.87 mW/cm2) operating at a wavelength of 800 nm. (b) Reproducibility of anti-Stokes luminescence generation during 17 on/off cycles over a period of 10000 s. Before measurements, effective optical charging of the probe can be done by UV-vis light for 5 min, the measurements were taken at the interval of 24 hours after charging.

Mentions: The stability and reproducibility of the anti-Stokes luminescence behavior of Zn3Ga2Ge2O10:Cr3+ are illustrated in Figure 4. Even after several hours of continuous irradiation with a LED (7.87 mW/cm2) operating at a wavelength of 800 nm, the anti-Stokes emission remains essentially unaltered in intensity (Ibackground/Ifluorescence ≈ 1/1000, Figure 4a). A similar result was obtained for cyclic operation over 17 individual on/off cycles of 10 min, where a maximum signal degradation of 8% was observed (Figure 4b). Both results indicate the high photo-stability of the material.


Anti-stokes fluorescent probe with incoherent excitation.

Li Y, Zhou S, Dong G, Peng M, Wondraczek L, Qiu J - Sci Rep (2014)

Anti-Stokes luminescence stability and reproducibility.(a) Evolution of luminescence monitored at 700 nm under continuous illumination with an incoherent LED (7.87 mW/cm2) operating at a wavelength of 800 nm. (b) Reproducibility of anti-Stokes luminescence generation during 17 on/off cycles over a period of 10000 s. Before measurements, effective optical charging of the probe can be done by UV-vis light for 5 min, the measurements were taken at the interval of 24 hours after charging.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921629&req=5

f4: Anti-Stokes luminescence stability and reproducibility.(a) Evolution of luminescence monitored at 700 nm under continuous illumination with an incoherent LED (7.87 mW/cm2) operating at a wavelength of 800 nm. (b) Reproducibility of anti-Stokes luminescence generation during 17 on/off cycles over a period of 10000 s. Before measurements, effective optical charging of the probe can be done by UV-vis light for 5 min, the measurements were taken at the interval of 24 hours after charging.
Mentions: The stability and reproducibility of the anti-Stokes luminescence behavior of Zn3Ga2Ge2O10:Cr3+ are illustrated in Figure 4. Even after several hours of continuous irradiation with a LED (7.87 mW/cm2) operating at a wavelength of 800 nm, the anti-Stokes emission remains essentially unaltered in intensity (Ibackground/Ifluorescence ≈ 1/1000, Figure 4a). A similar result was obtained for cyclic operation over 17 individual on/off cycles of 10 min, where a maximum signal degradation of 8% was observed (Figure 4b). Both results indicate the high photo-stability of the material.

Bottom Line: Although inorganic anti-Stokes fluorescent probes have long been developed, the operational mode of today's most advanced examples still involves the harsh requirement of coherent laser excitation, which often yields unexpected light disturbance or even photon-induced deterioration during optical imaging.We show that the probe can be operated under light-emitting diode excitation and provides tunable anti-Stokes energy shift and decay kinetics, which allow for rapid and deep tissue imaging over a very large area with negligible photodestruction.Charging of the probe can be achieved by either X-rays or ultraviolet-visible light irradiation, which enables multiplexed detection and function integration with standard X-ray medical imaging devices.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Luminescent Materials and Devices, Institute of Optical Communication Materials, School of Materials Science and Technology, South China University of Technology, Guangzhou 510640, China.

ABSTRACT
Although inorganic anti-Stokes fluorescent probes have long been developed, the operational mode of today's most advanced examples still involves the harsh requirement of coherent laser excitation, which often yields unexpected light disturbance or even photon-induced deterioration during optical imaging. Here, we demonstrate an efficient anti-Stokes fluorescent probe with incoherent excitation. We show that the probe can be operated under light-emitting diode excitation and provides tunable anti-Stokes energy shift and decay kinetics, which allow for rapid and deep tissue imaging over a very large area with negligible photodestruction. Charging of the probe can be achieved by either X-rays or ultraviolet-visible light irradiation, which enables multiplexed detection and function integration with standard X-ray medical imaging devices.

Show MeSH
Related in: MedlinePlus