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Vibrio diversity and dynamics in the Monterey Bay upwelling region.

Mansergh S, Zehr JP - Front Microbiol (2014)

Bottom Line: Total Vibrio populations showed some seasonal variability but greater variability was observed within the two subpopulations.MBAY Vib7 was also negatively associated with upwelling indices and represented a deeper Vibrio sp. population.This study is the first to target and describe the diversity and dynamics of these natural populations in MB and demonstrates that these populations shift seasonally within the region.

View Article: PubMed Central - PubMed

Affiliation: Ocean Sciences Department, University of California at Santa Cruz Santa Cruz, CA, USA.

ABSTRACT
The Vibrionaceae (Vibrio) are a ubiquitous group of metabolically flexible marine bacteria that play important roles in biogeochemical cycling in the ocean. Despite this versatility, little is known about Vibrio diversity and abundances in upwelling regions. The seasonal dynamics of Vibrio populations was examined by analysis of 16S rRNA genes in Monterey Bay (MB), California from April 2006-April 2008 at two long term monitoring stations, C1 and M2. Vibrio phylotypes within MB were diverse, with subpopulations clustering with several different cultured representatives including Allivibrio spp., Vibrio penaecida, and Vibrio splendidus as well as with many unidentified marine environmental bacterial 16S rRNA gene sequences. Total Vibrio population abundances, as well as abundances of a Vibrio sp. subpopulation (MBAY Vib7) and an Allivibrio sp. subpopulation (MBAY Vib4) were examined in the context of environmental parameters from mooring station and CTD cast data. Total Vibrio populations showed some seasonal variability but greater variability was observed within the two subpopulations. MBAY Vib4 was negatively associated with MB upwelling indices and positively correlated with oceanic season conditions, when upwelling winds relax and warmer surface waters are present in MB. MBAY Vib7 was also negatively associated with upwelling indices and represented a deeper Vibrio sp. population. Correlation patterns suggest that larger oceanographic conditions affect the dynamics of the populations in MB, rather than specific environmental factors. This study is the first to target and describe the diversity and dynamics of these natural populations in MB and demonstrates that these populations shift seasonally within the region.

No MeSH data available.


Related in: MedlinePlus

Monthly Upwelling Index (m3 s-1 100 m of coastline-1), temperature (°C), salinity (ppt), chlorophyll a (μg m−3) and total Vibrio, MBAY Vib4 and MBAY Vib7 concentrations (natural log of gene copies liter−1) for stations C1 (A) and M2 (B). Black dots represent discrete samples within the CTD cast.
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Figure 4: Monthly Upwelling Index (m3 s-1 100 m of coastline-1), temperature (°C), salinity (ppt), chlorophyll a (μg m−3) and total Vibrio, MBAY Vib4 and MBAY Vib7 concentrations (natural log of gene copies liter−1) for stations C1 (A) and M2 (B). Black dots represent discrete samples within the CTD cast.

Mentions: There were observed differences in the Vibrio populations at both of the stations, with the MBAY Vib4 group having higher peak abundances at station C1 (Figure 4). The MBAY Vib4 group ranged in concentration from undetectable levels at all depths and stations up to 1.95 × 106 gene copies liter−1 seawater at 5 m at station C1 (Table 2). MBAY Vib4 was positively correlated to temperature (ρs = 0.32, p = 0.002), oceanic season (ρs = 0.41, p = 0.002), Synechococcus (ρs = 0.20, p = 0.02), and total Vibrio abundance (ρs = 0.55, p < 0.001) (Table 3). This group was negatively correlated to salinity (ρs = −0.22, p = 0.02), chlorophyll a (ρs = −0.16, p = 0.035), monthly UI (ρs = −0.61, p < 0.001), surface phytoplankton (ρs = −0.44, p < 0.001), and total diatoms (ρs = −0.55, p < 0.001) (Table 3).


Vibrio diversity and dynamics in the Monterey Bay upwelling region.

Mansergh S, Zehr JP - Front Microbiol (2014)

Monthly Upwelling Index (m3 s-1 100 m of coastline-1), temperature (°C), salinity (ppt), chlorophyll a (μg m−3) and total Vibrio, MBAY Vib4 and MBAY Vib7 concentrations (natural log of gene copies liter−1) for stations C1 (A) and M2 (B). Black dots represent discrete samples within the CTD cast.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921578&req=5

Figure 4: Monthly Upwelling Index (m3 s-1 100 m of coastline-1), temperature (°C), salinity (ppt), chlorophyll a (μg m−3) and total Vibrio, MBAY Vib4 and MBAY Vib7 concentrations (natural log of gene copies liter−1) for stations C1 (A) and M2 (B). Black dots represent discrete samples within the CTD cast.
Mentions: There were observed differences in the Vibrio populations at both of the stations, with the MBAY Vib4 group having higher peak abundances at station C1 (Figure 4). The MBAY Vib4 group ranged in concentration from undetectable levels at all depths and stations up to 1.95 × 106 gene copies liter−1 seawater at 5 m at station C1 (Table 2). MBAY Vib4 was positively correlated to temperature (ρs = 0.32, p = 0.002), oceanic season (ρs = 0.41, p = 0.002), Synechococcus (ρs = 0.20, p = 0.02), and total Vibrio abundance (ρs = 0.55, p < 0.001) (Table 3). This group was negatively correlated to salinity (ρs = −0.22, p = 0.02), chlorophyll a (ρs = −0.16, p = 0.035), monthly UI (ρs = −0.61, p < 0.001), surface phytoplankton (ρs = −0.44, p < 0.001), and total diatoms (ρs = −0.55, p < 0.001) (Table 3).

Bottom Line: Total Vibrio populations showed some seasonal variability but greater variability was observed within the two subpopulations.MBAY Vib7 was also negatively associated with upwelling indices and represented a deeper Vibrio sp. population.This study is the first to target and describe the diversity and dynamics of these natural populations in MB and demonstrates that these populations shift seasonally within the region.

View Article: PubMed Central - PubMed

Affiliation: Ocean Sciences Department, University of California at Santa Cruz Santa Cruz, CA, USA.

ABSTRACT
The Vibrionaceae (Vibrio) are a ubiquitous group of metabolically flexible marine bacteria that play important roles in biogeochemical cycling in the ocean. Despite this versatility, little is known about Vibrio diversity and abundances in upwelling regions. The seasonal dynamics of Vibrio populations was examined by analysis of 16S rRNA genes in Monterey Bay (MB), California from April 2006-April 2008 at two long term monitoring stations, C1 and M2. Vibrio phylotypes within MB were diverse, with subpopulations clustering with several different cultured representatives including Allivibrio spp., Vibrio penaecida, and Vibrio splendidus as well as with many unidentified marine environmental bacterial 16S rRNA gene sequences. Total Vibrio population abundances, as well as abundances of a Vibrio sp. subpopulation (MBAY Vib7) and an Allivibrio sp. subpopulation (MBAY Vib4) were examined in the context of environmental parameters from mooring station and CTD cast data. Total Vibrio populations showed some seasonal variability but greater variability was observed within the two subpopulations. MBAY Vib4 was negatively associated with MB upwelling indices and positively correlated with oceanic season conditions, when upwelling winds relax and warmer surface waters are present in MB. MBAY Vib7 was also negatively associated with upwelling indices and represented a deeper Vibrio sp. population. Correlation patterns suggest that larger oceanographic conditions affect the dynamics of the populations in MB, rather than specific environmental factors. This study is the first to target and describe the diversity and dynamics of these natural populations in MB and demonstrates that these populations shift seasonally within the region.

No MeSH data available.


Related in: MedlinePlus