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NT5E and FcGBP as key regulators of TGF-1-induced epithelial-mesenchymal transition (EMT) are associated with tumor progression and survival of patients with gallbladder cancer.

Xiong L, Wen Y, Miao X, Yang Z - Cell Tissue Res. (2013)

Bottom Line: In the present study, we used DNA micro-array analysis to search for differentially expressed genes in the TGF-β1 induced gallbladder carcinoma cell line (GBC-SD cells), as compared with normal GBC-SD cells.NT5E (CD73) is the most increased gene, while the Fc fragment of the IgG binding protein (FcGBP) is the most decreased gene.Immunochemistry and clinicopathological results showed that the expression of NT5E and FcGBP in gallbladder adenocarcinoma is an independent marker for evaluation of the disease progression, clinical biological behaviors and prognosis.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, 139# Middle Renmin road, Changsha, Hunan, 410011, China.

ABSTRACT
Epithelial-mesenchymal transitions (EMTs) are essential manifestations of epithelial cell plasticity during tumor progression. Transforming growth factor-β(TGF-β) modulates epithelial plasticity in tumor physiological contexts by inducing EMT, which is associated with the altered expression of genes. In the present study, we used DNA micro-array analysis to search for differentially expressed genes in the TGF-β1 induced gallbladder carcinoma cell line (GBC-SD cells), as compared with normal GBC-SD cells. We identified 225 differentially expressed genes, including 144 that were over-expressed and 81 that were under-expressed in the TGF-β1 induced GBC-SD cells. NT5E (CD73) is the most increased gene, while the Fc fragment of the IgG binding protein (FcGBP) is the most decreased gene. The expression patterns of these two genes in gallbladder adenocarcinoma and chronic cholecystitis tissue were consistent with the micro-array data. Immunochemistry and clinicopathological results showed that the expression of NT5E and FcGBP in gallbladder adenocarcinoma is an independent marker for evaluation of the disease progression, clinical biological behaviors and prognosis. The data from the current study indicate that differential NT5E and FcGBP expressions could be further evaluated as biomarkers for predicting survival of patients with gallbladder cancer and that NT5E and FcGBP could be promising targets in the control of gallbladder cancer progression.

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siRNA-mediated knockdown of NT5E affected cell migration by Transwell assay. a GBC-SD cells transfected with pRNA-siNT5E-1. b GBC-SD cells transfected with unrelated sequence vector. c Non-transfected group. Cell numbers in three microscope fields (×200) from each Transwell were counted. Columns, mean of migrating cells done in triplicate; **P < 0.01 compared to transfected with unrelated sequence vector or non-transfected group. Bars 100 μm
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Fig5: siRNA-mediated knockdown of NT5E affected cell migration by Transwell assay. a GBC-SD cells transfected with pRNA-siNT5E-1. b GBC-SD cells transfected with unrelated sequence vector. c Non-transfected group. Cell numbers in three microscope fields (×200) from each Transwell were counted. Columns, mean of migrating cells done in triplicate; **P < 0.01 compared to transfected with unrelated sequence vector or non-transfected group. Bars 100 μm

Mentions: To further confirm the role of NT5E in regulating cell motility, a transwell chamber invasion assay was used. Consistent with the results from the wound assay, Fig. 5 shows that the number of GBC-SD cells that were transfected with pRNA -siNT5E-1 that passed through the membrane was lower than that of the unrelated sequence vector transfected group and non-transfected GBC-SD cells; the difference was statistically significant (P < 0.05), while there was no statistically significant difference between the unrelated sequence vector transfected group and non-transfected group cells (P > 0.05).Fig. 5


NT5E and FcGBP as key regulators of TGF-1-induced epithelial-mesenchymal transition (EMT) are associated with tumor progression and survival of patients with gallbladder cancer.

Xiong L, Wen Y, Miao X, Yang Z - Cell Tissue Res. (2013)

siRNA-mediated knockdown of NT5E affected cell migration by Transwell assay. a GBC-SD cells transfected with pRNA-siNT5E-1. b GBC-SD cells transfected with unrelated sequence vector. c Non-transfected group. Cell numbers in three microscope fields (×200) from each Transwell were counted. Columns, mean of migrating cells done in triplicate; **P < 0.01 compared to transfected with unrelated sequence vector or non-transfected group. Bars 100 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3921456&req=5

Fig5: siRNA-mediated knockdown of NT5E affected cell migration by Transwell assay. a GBC-SD cells transfected with pRNA-siNT5E-1. b GBC-SD cells transfected with unrelated sequence vector. c Non-transfected group. Cell numbers in three microscope fields (×200) from each Transwell were counted. Columns, mean of migrating cells done in triplicate; **P < 0.01 compared to transfected with unrelated sequence vector or non-transfected group. Bars 100 μm
Mentions: To further confirm the role of NT5E in regulating cell motility, a transwell chamber invasion assay was used. Consistent with the results from the wound assay, Fig. 5 shows that the number of GBC-SD cells that were transfected with pRNA -siNT5E-1 that passed through the membrane was lower than that of the unrelated sequence vector transfected group and non-transfected GBC-SD cells; the difference was statistically significant (P < 0.05), while there was no statistically significant difference between the unrelated sequence vector transfected group and non-transfected group cells (P > 0.05).Fig. 5

Bottom Line: In the present study, we used DNA micro-array analysis to search for differentially expressed genes in the TGF-β1 induced gallbladder carcinoma cell line (GBC-SD cells), as compared with normal GBC-SD cells.NT5E (CD73) is the most increased gene, while the Fc fragment of the IgG binding protein (FcGBP) is the most decreased gene.Immunochemistry and clinicopathological results showed that the expression of NT5E and FcGBP in gallbladder adenocarcinoma is an independent marker for evaluation of the disease progression, clinical biological behaviors and prognosis.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, 139# Middle Renmin road, Changsha, Hunan, 410011, China.

ABSTRACT
Epithelial-mesenchymal transitions (EMTs) are essential manifestations of epithelial cell plasticity during tumor progression. Transforming growth factor-β(TGF-β) modulates epithelial plasticity in tumor physiological contexts by inducing EMT, which is associated with the altered expression of genes. In the present study, we used DNA micro-array analysis to search for differentially expressed genes in the TGF-β1 induced gallbladder carcinoma cell line (GBC-SD cells), as compared with normal GBC-SD cells. We identified 225 differentially expressed genes, including 144 that were over-expressed and 81 that were under-expressed in the TGF-β1 induced GBC-SD cells. NT5E (CD73) is the most increased gene, while the Fc fragment of the IgG binding protein (FcGBP) is the most decreased gene. The expression patterns of these two genes in gallbladder adenocarcinoma and chronic cholecystitis tissue were consistent with the micro-array data. Immunochemistry and clinicopathological results showed that the expression of NT5E and FcGBP in gallbladder adenocarcinoma is an independent marker for evaluation of the disease progression, clinical biological behaviors and prognosis. The data from the current study indicate that differential NT5E and FcGBP expressions could be further evaluated as biomarkers for predicting survival of patients with gallbladder cancer and that NT5E and FcGBP could be promising targets in the control of gallbladder cancer progression.

Show MeSH
Related in: MedlinePlus