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NT5E and FcGBP as key regulators of TGF-1-induced epithelial-mesenchymal transition (EMT) are associated with tumor progression and survival of patients with gallbladder cancer.

Xiong L, Wen Y, Miao X, Yang Z - Cell Tissue Res. (2013)

Bottom Line: In the present study, we used DNA micro-array analysis to search for differentially expressed genes in the TGF-β1 induced gallbladder carcinoma cell line (GBC-SD cells), as compared with normal GBC-SD cells.NT5E (CD73) is the most increased gene, while the Fc fragment of the IgG binding protein (FcGBP) is the most decreased gene.Immunochemistry and clinicopathological results showed that the expression of NT5E and FcGBP in gallbladder adenocarcinoma is an independent marker for evaluation of the disease progression, clinical biological behaviors and prognosis.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, 139# Middle Renmin road, Changsha, Hunan, 410011, China.

ABSTRACT
Epithelial-mesenchymal transitions (EMTs) are essential manifestations of epithelial cell plasticity during tumor progression. Transforming growth factor-β(TGF-β) modulates epithelial plasticity in tumor physiological contexts by inducing EMT, which is associated with the altered expression of genes. In the present study, we used DNA micro-array analysis to search for differentially expressed genes in the TGF-β1 induced gallbladder carcinoma cell line (GBC-SD cells), as compared with normal GBC-SD cells. We identified 225 differentially expressed genes, including 144 that were over-expressed and 81 that were under-expressed in the TGF-β1 induced GBC-SD cells. NT5E (CD73) is the most increased gene, while the Fc fragment of the IgG binding protein (FcGBP) is the most decreased gene. The expression patterns of these two genes in gallbladder adenocarcinoma and chronic cholecystitis tissue were consistent with the micro-array data. Immunochemistry and clinicopathological results showed that the expression of NT5E and FcGBP in gallbladder adenocarcinoma is an independent marker for evaluation of the disease progression, clinical biological behaviors and prognosis. The data from the current study indicate that differential NT5E and FcGBP expressions could be further evaluated as biomarkers for predicting survival of patients with gallbladder cancer and that NT5E and FcGBP could be promising targets in the control of gallbladder cancer progression.

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Transfected with pRNA-siNT5E-1 induced an increase in VIM mRNA and a decrease in CDH1 mRNA in GBC-SD cells. S: GBC-SD cells transfected with pRNA-siNT5E-1; C1: GBC-SD cells transfected with unrelated sequence vector; C2: non-transfected group. NT5E and VIM gene (**P < 0.01 compared to transfected with unrelated sequence vector or non-transfected group)
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Fig2: Transfected with pRNA-siNT5E-1 induced an increase in VIM mRNA and a decrease in CDH1 mRNA in GBC-SD cells. S: GBC-SD cells transfected with pRNA-siNT5E-1; C1: GBC-SD cells transfected with unrelated sequence vector; C2: non-transfected group. NT5E and VIM gene (**P < 0.01 compared to transfected with unrelated sequence vector or non-transfected group)

Mentions: We examined the effect of NT5E on the expression of key regulators of EMT including CDH1 and VIM. Real-time PCR analysis indicated that the expression of NT5E and VIM gene in the GBC-SD cells transfected with pRNA-siNT5E-1 was significantly lower than in the unrelated sequence vector transfected group and non-transfected group (P < 0.01), while the expression level of CDH1 gene in the GBC-SD cells transfected with the pRNA-siNT5E-1 vector was significantly higher than others (P < 0.01), as shown in Fig. 2. The expression of NT5E , CDH1 and VIM mRNA had no statistical significance between the group that was transfected with the unrelated sequence vector and the non-transfected group (P > 0.05).Fig. 2


NT5E and FcGBP as key regulators of TGF-1-induced epithelial-mesenchymal transition (EMT) are associated with tumor progression and survival of patients with gallbladder cancer.

Xiong L, Wen Y, Miao X, Yang Z - Cell Tissue Res. (2013)

Transfected with pRNA-siNT5E-1 induced an increase in VIM mRNA and a decrease in CDH1 mRNA in GBC-SD cells. S: GBC-SD cells transfected with pRNA-siNT5E-1; C1: GBC-SD cells transfected with unrelated sequence vector; C2: non-transfected group. NT5E and VIM gene (**P < 0.01 compared to transfected with unrelated sequence vector or non-transfected group)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3921456&req=5

Fig2: Transfected with pRNA-siNT5E-1 induced an increase in VIM mRNA and a decrease in CDH1 mRNA in GBC-SD cells. S: GBC-SD cells transfected with pRNA-siNT5E-1; C1: GBC-SD cells transfected with unrelated sequence vector; C2: non-transfected group. NT5E and VIM gene (**P < 0.01 compared to transfected with unrelated sequence vector or non-transfected group)
Mentions: We examined the effect of NT5E on the expression of key regulators of EMT including CDH1 and VIM. Real-time PCR analysis indicated that the expression of NT5E and VIM gene in the GBC-SD cells transfected with pRNA-siNT5E-1 was significantly lower than in the unrelated sequence vector transfected group and non-transfected group (P < 0.01), while the expression level of CDH1 gene in the GBC-SD cells transfected with the pRNA-siNT5E-1 vector was significantly higher than others (P < 0.01), as shown in Fig. 2. The expression of NT5E , CDH1 and VIM mRNA had no statistical significance between the group that was transfected with the unrelated sequence vector and the non-transfected group (P > 0.05).Fig. 2

Bottom Line: In the present study, we used DNA micro-array analysis to search for differentially expressed genes in the TGF-β1 induced gallbladder carcinoma cell line (GBC-SD cells), as compared with normal GBC-SD cells.NT5E (CD73) is the most increased gene, while the Fc fragment of the IgG binding protein (FcGBP) is the most decreased gene.Immunochemistry and clinicopathological results showed that the expression of NT5E and FcGBP in gallbladder adenocarcinoma is an independent marker for evaluation of the disease progression, clinical biological behaviors and prognosis.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, 139# Middle Renmin road, Changsha, Hunan, 410011, China.

ABSTRACT
Epithelial-mesenchymal transitions (EMTs) are essential manifestations of epithelial cell plasticity during tumor progression. Transforming growth factor-β(TGF-β) modulates epithelial plasticity in tumor physiological contexts by inducing EMT, which is associated with the altered expression of genes. In the present study, we used DNA micro-array analysis to search for differentially expressed genes in the TGF-β1 induced gallbladder carcinoma cell line (GBC-SD cells), as compared with normal GBC-SD cells. We identified 225 differentially expressed genes, including 144 that were over-expressed and 81 that were under-expressed in the TGF-β1 induced GBC-SD cells. NT5E (CD73) is the most increased gene, while the Fc fragment of the IgG binding protein (FcGBP) is the most decreased gene. The expression patterns of these two genes in gallbladder adenocarcinoma and chronic cholecystitis tissue were consistent with the micro-array data. Immunochemistry and clinicopathological results showed that the expression of NT5E and FcGBP in gallbladder adenocarcinoma is an independent marker for evaluation of the disease progression, clinical biological behaviors and prognosis. The data from the current study indicate that differential NT5E and FcGBP expressions could be further evaluated as biomarkers for predicting survival of patients with gallbladder cancer and that NT5E and FcGBP could be promising targets in the control of gallbladder cancer progression.

Show MeSH
Related in: MedlinePlus