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Effects of costimulation on intrahepatic immunopathogenesis in patients with chronic HBV infection.

Zhong B, Huang MP, Yin GQ, Gao X - Inflamm. Res. (2013)

Bottom Line: The ACs presented decreased inflammatory and increased inhibitory costimulation, which was negatively correlated with inflammatory costimulatory proteins and ALT, whereas the ACH patients exhibited increased inflammatory costimulation and decreased inhibitory costimulation, which was correlated with increased ALT.The Cir patients showed both increased inhibitory and inflammatory costimulation.The HCC patients exhibited both decreased inhibitory and inflammatory costimulation.

View Article: PubMed Central - PubMed

Affiliation: The Affiliated Qingyuan Hospital, Jinan University Medical School, Yinquannan Road, Qingyuan, 511500, Guangdong, China, zhongbei62@sina.com.

ABSTRACT

Objective: Chronic HBV infection can lead to "immune tolerance" in asymptomatic carriers (ACs), "immune injury" in active chronic hepatitis (ACH) patients or "immune abnormality" in cirrhosis (Cir) and hepatocellular carcinoma (HCC) patients. Previous investigations reported that chronic hepatitis presented abnormal expression of costimulatory molecules. We investigated the costimulation profile in the liver of ACs and patients with ACH, Cir and HCC.

Methods: Patients with ACH, Cir and HCC, ACs and normal controls were recruited into the present study. The costimulation profiles and cytokines in the liver of patients were investigated by Western blotting, immunohistochemistry and real-time quantitative PCR. Correlations between serum alanime aminotransferase (ALT) levels, necroinflammation scores, cytokines and costimulatory proteins were assessed.

Results: The ACs presented decreased inflammatory and increased inhibitory costimulation, which was negatively correlated with inflammatory costimulatory proteins and ALT, whereas the ACH patients exhibited increased inflammatory costimulation and decreased inhibitory costimulation, which was correlated with increased ALT. The Cir patients showed both increased inhibitory and inflammatory costimulation. The HCC patients exhibited both decreased inhibitory and inflammatory costimulation.

Conclusion: Costimulation participates in intrahepatic immune responses, and plays important roles in immune tolerance, immune injury and immune abnormalities in patients with chronic HBV infection.

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Related in: MedlinePlus

Correlation between intrahepatic scores of necroinflammation and serum ALT, TB, HBsAg or HBV-DNA load. The histological examination of liver tissues was performed in 24 CHB patients, 6 patients in each group. The intrahepatic scores of necroinflammation were graded by the system of Ishak modified HAI. The correlation coefficient between the scores of necroinflammation and ALT, TB, HBsAg or HBV-DNA load was analyzed. The scores of necroinflammation in liver were positively correlated with ALT and TB, but negatively correlated with HBsAg and HBVDNA load in all the CHB patients
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Fig1: Correlation between intrahepatic scores of necroinflammation and serum ALT, TB, HBsAg or HBV-DNA load. The histological examination of liver tissues was performed in 24 CHB patients, 6 patients in each group. The intrahepatic scores of necroinflammation were graded by the system of Ishak modified HAI. The correlation coefficient between the scores of necroinflammation and ALT, TB, HBsAg or HBV-DNA load was analyzed. The scores of necroinflammation in liver were positively correlated with ALT and TB, but negatively correlated with HBsAg and HBVDNA load in all the CHB patients

Mentions: On correlation analysis, necroinflammatory scores in liver were positively correlated with ALT and total bilirubin (TB), but negatively correlated with HBsAg and HBV-DNA load in all CHB patients (Fig. 1). The results shown in Fig. 1 suggest that increased ALT and TB in peripheral blood and the severity of necroinflammation in liver were closely interconnected, and that high inflammatory responses partially inhibited HBV replication.Fig. 1


Effects of costimulation on intrahepatic immunopathogenesis in patients with chronic HBV infection.

Zhong B, Huang MP, Yin GQ, Gao X - Inflamm. Res. (2013)

Correlation between intrahepatic scores of necroinflammation and serum ALT, TB, HBsAg or HBV-DNA load. The histological examination of liver tissues was performed in 24 CHB patients, 6 patients in each group. The intrahepatic scores of necroinflammation were graded by the system of Ishak modified HAI. The correlation coefficient between the scores of necroinflammation and ALT, TB, HBsAg or HBV-DNA load was analyzed. The scores of necroinflammation in liver were positively correlated with ALT and TB, but negatively correlated with HBsAg and HBVDNA load in all the CHB patients
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3921453&req=5

Fig1: Correlation between intrahepatic scores of necroinflammation and serum ALT, TB, HBsAg or HBV-DNA load. The histological examination of liver tissues was performed in 24 CHB patients, 6 patients in each group. The intrahepatic scores of necroinflammation were graded by the system of Ishak modified HAI. The correlation coefficient between the scores of necroinflammation and ALT, TB, HBsAg or HBV-DNA load was analyzed. The scores of necroinflammation in liver were positively correlated with ALT and TB, but negatively correlated with HBsAg and HBVDNA load in all the CHB patients
Mentions: On correlation analysis, necroinflammatory scores in liver were positively correlated with ALT and total bilirubin (TB), but negatively correlated with HBsAg and HBV-DNA load in all CHB patients (Fig. 1). The results shown in Fig. 1 suggest that increased ALT and TB in peripheral blood and the severity of necroinflammation in liver were closely interconnected, and that high inflammatory responses partially inhibited HBV replication.Fig. 1

Bottom Line: The ACs presented decreased inflammatory and increased inhibitory costimulation, which was negatively correlated with inflammatory costimulatory proteins and ALT, whereas the ACH patients exhibited increased inflammatory costimulation and decreased inhibitory costimulation, which was correlated with increased ALT.The Cir patients showed both increased inhibitory and inflammatory costimulation.The HCC patients exhibited both decreased inhibitory and inflammatory costimulation.

View Article: PubMed Central - PubMed

Affiliation: The Affiliated Qingyuan Hospital, Jinan University Medical School, Yinquannan Road, Qingyuan, 511500, Guangdong, China, zhongbei62@sina.com.

ABSTRACT

Objective: Chronic HBV infection can lead to "immune tolerance" in asymptomatic carriers (ACs), "immune injury" in active chronic hepatitis (ACH) patients or "immune abnormality" in cirrhosis (Cir) and hepatocellular carcinoma (HCC) patients. Previous investigations reported that chronic hepatitis presented abnormal expression of costimulatory molecules. We investigated the costimulation profile in the liver of ACs and patients with ACH, Cir and HCC.

Methods: Patients with ACH, Cir and HCC, ACs and normal controls were recruited into the present study. The costimulation profiles and cytokines in the liver of patients were investigated by Western blotting, immunohistochemistry and real-time quantitative PCR. Correlations between serum alanime aminotransferase (ALT) levels, necroinflammation scores, cytokines and costimulatory proteins were assessed.

Results: The ACs presented decreased inflammatory and increased inhibitory costimulation, which was negatively correlated with inflammatory costimulatory proteins and ALT, whereas the ACH patients exhibited increased inflammatory costimulation and decreased inhibitory costimulation, which was correlated with increased ALT. The Cir patients showed both increased inhibitory and inflammatory costimulation. The HCC patients exhibited both decreased inhibitory and inflammatory costimulation.

Conclusion: Costimulation participates in intrahepatic immune responses, and plays important roles in immune tolerance, immune injury and immune abnormalities in patients with chronic HBV infection.

Show MeSH
Related in: MedlinePlus