Limits...
Identification of more than two paternal haplotypes of the ovine fatty acid-binding protein 4 (FABP4) gene in half-sib families: evidence of intragenic meiotic recombination.

Yan W, Zhou H, Luo Y, Hu J, Hickford JG - PLoS ONE (2014)

Bottom Line: Across these regions, fourteen haplotypes have been identified.Between three and four paternally-derived haplotypes were identified in the progeny of six of the seven sires, suggesting that meiotic recombination occurs within ovine FABP4.A number of sequence motifs associated with recombination "hotspots" were detected in the two regions of the gene that were analyzed and these may facilitate the recombination.

View Article: PubMed Central - PubMed

Affiliation: Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.

ABSTRACT
The fatty acid binding protein 4 (FABP4) plays an important role in the regulation of lipid metabolism in mammals. In this study, two regions of ovine FABP4 spanning exon 2-intron 2 and exon 3-intron 3 were investigated in four hundred and twenty lambs derived from seven sires that were previously typed as having heterozygous genotypes in both these regions of the gene. These regions have been shown to be variable, with three SNPs plus one indel and four SNPs respectively constituting five and four allele variants in the two regions. Across these regions, fourteen haplotypes have been identified. The lambs were typed using a Polymerase Chain Reaction Single-Stranded Conformational Polymorphism (PCR-SSCP) method to identify the haplotypes inherited from the sires. Between three and four paternally-derived haplotypes were identified in the progeny of six of the seven sires, suggesting that meiotic recombination occurs within ovine FABP4. A number of sequence motifs associated with recombination "hotspots" were detected in the two regions of the gene that were analyzed and these may facilitate the recombination.

Show MeSH

Related in: MedlinePlus

The presence of potential “recombination hotspots” in ovine FABP4 and the haplotypes across two gene regions that were inherited from the seven NZ Romney sires.The locations of these two regions in ovine FABP4 are indicated. An unfilled triangle represents uble overlapping chi-like sequences (GCTGGTGCTGGTGA), and a horizontal line filled triangle represents the CRE-like sequence (ATGAAGTCA). The CCTCCCT motifs and variants are indicated by dot filled triangles, and the CCAAT motifs are shown as filled triangles. The numbering of nucleotide positions follows the nomenclature described at www.hgvs.org/mutnomen/
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3921221&req=5

pone-0088691-g002: The presence of potential “recombination hotspots” in ovine FABP4 and the haplotypes across two gene regions that were inherited from the seven NZ Romney sires.The locations of these two regions in ovine FABP4 are indicated. An unfilled triangle represents uble overlapping chi-like sequences (GCTGGTGCTGGTGA), and a horizontal line filled triangle represents the CRE-like sequence (ATGAAGTCA). The CCTCCCT motifs and variants are indicated by dot filled triangles, and the CCAAT motifs are shown as filled triangles. The numbering of nucleotide positions follows the nomenclature described at www.hgvs.org/mutnomen/

Mentions: Within the ovine FABP4 regions analysed, we found a number of simple sequence motifs that have been reported to be associated with recombination hotspots in both prokaryotes and eukaryotes. These included: 1) a 14-mer sequence (GCTGGTGCTGGTGA) consisting of two partially overlapping chi-like sequences (GCTGGTGC and GCTGGTGA) in region 2; 2) a CRE-like sequence (ATGAAGTCA) in region 1; 3) a CCTCCCT motif approximately 2 kb upstream of region 1 and variants of this sequence in region 1 and region 2; and 4) a number of copies of a CCAAT motif in region 1, region 2, and other regions of the gene (Figure 2).


Identification of more than two paternal haplotypes of the ovine fatty acid-binding protein 4 (FABP4) gene in half-sib families: evidence of intragenic meiotic recombination.

Yan W, Zhou H, Luo Y, Hu J, Hickford JG - PLoS ONE (2014)

The presence of potential “recombination hotspots” in ovine FABP4 and the haplotypes across two gene regions that were inherited from the seven NZ Romney sires.The locations of these two regions in ovine FABP4 are indicated. An unfilled triangle represents uble overlapping chi-like sequences (GCTGGTGCTGGTGA), and a horizontal line filled triangle represents the CRE-like sequence (ATGAAGTCA). The CCTCCCT motifs and variants are indicated by dot filled triangles, and the CCAAT motifs are shown as filled triangles. The numbering of nucleotide positions follows the nomenclature described at www.hgvs.org/mutnomen/
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921221&req=5

pone-0088691-g002: The presence of potential “recombination hotspots” in ovine FABP4 and the haplotypes across two gene regions that were inherited from the seven NZ Romney sires.The locations of these two regions in ovine FABP4 are indicated. An unfilled triangle represents uble overlapping chi-like sequences (GCTGGTGCTGGTGA), and a horizontal line filled triangle represents the CRE-like sequence (ATGAAGTCA). The CCTCCCT motifs and variants are indicated by dot filled triangles, and the CCAAT motifs are shown as filled triangles. The numbering of nucleotide positions follows the nomenclature described at www.hgvs.org/mutnomen/
Mentions: Within the ovine FABP4 regions analysed, we found a number of simple sequence motifs that have been reported to be associated with recombination hotspots in both prokaryotes and eukaryotes. These included: 1) a 14-mer sequence (GCTGGTGCTGGTGA) consisting of two partially overlapping chi-like sequences (GCTGGTGC and GCTGGTGA) in region 2; 2) a CRE-like sequence (ATGAAGTCA) in region 1; 3) a CCTCCCT motif approximately 2 kb upstream of region 1 and variants of this sequence in region 1 and region 2; and 4) a number of copies of a CCAAT motif in region 1, region 2, and other regions of the gene (Figure 2).

Bottom Line: Across these regions, fourteen haplotypes have been identified.Between three and four paternally-derived haplotypes were identified in the progeny of six of the seven sires, suggesting that meiotic recombination occurs within ovine FABP4.A number of sequence motifs associated with recombination "hotspots" were detected in the two regions of the gene that were analyzed and these may facilitate the recombination.

View Article: PubMed Central - PubMed

Affiliation: Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.

ABSTRACT
The fatty acid binding protein 4 (FABP4) plays an important role in the regulation of lipid metabolism in mammals. In this study, two regions of ovine FABP4 spanning exon 2-intron 2 and exon 3-intron 3 were investigated in four hundred and twenty lambs derived from seven sires that were previously typed as having heterozygous genotypes in both these regions of the gene. These regions have been shown to be variable, with three SNPs plus one indel and four SNPs respectively constituting five and four allele variants in the two regions. Across these regions, fourteen haplotypes have been identified. The lambs were typed using a Polymerase Chain Reaction Single-Stranded Conformational Polymorphism (PCR-SSCP) method to identify the haplotypes inherited from the sires. Between three and four paternally-derived haplotypes were identified in the progeny of six of the seven sires, suggesting that meiotic recombination occurs within ovine FABP4. A number of sequence motifs associated with recombination "hotspots" were detected in the two regions of the gene that were analyzed and these may facilitate the recombination.

Show MeSH
Related in: MedlinePlus