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TFIIB-related factor 2 over expression is a prognosis marker for early-stage non-small cell lung cancer correlated with tumor angiogenesis.

Lu M, Tian H, Yue W, Li L, Li S, Qi L, Hu W, Gao C, Si L - PLoS ONE (2014)

Bottom Line: Intratumoral microvessel density (MVD) was measured by counting CD-34 positive immunostained endothelial cells.In addition, univariate and multivariate analysis demonstrated that BRF2 protein over-expression and high MVD were significantly associated with tumor relapse.Although BRF2 overexpression and high MVD indicated poor 5-year overall survival (p = 0.004 and p = 0.019, respectively), multivariate analysis demonstrated that only BRF2 overexpression was an independent prognostic factor for unfavorable overall survival (P = 0.021).

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic Surgery, Qilu Hospital, Shandong University, Jinan, P. R. China.

ABSTRACT

Background: The aim of this study was to examine BRF2 expression in patients with non-small cell lung cancer (NSCLC) and explore the relationship of BRF2 protein with clinicopathologic factors, tumor angiogenesis and prognosis.

Methods: Both BRF2 protein and intratumoral microvessels were examined by immunohistochemical staining in 107 non-small cell lung cancer patients. Intratumoral microvessel density (MVD) was measured by counting CD-34 positive immunostained endothelial cells. Western blot and RT-PCR analyses were utilized to investigate the BRF2 expression status in tissues.

Results: A notably higher level of BRF2 expression was found in NSCLC tissues at protein levels. In addition, univariate and multivariate analysis demonstrated that BRF2 protein over-expression and high MVD were significantly associated with tumor relapse. Although BRF2 overexpression and high MVD indicated poor 5-year overall survival (p = 0.004 and p = 0.019, respectively), multivariate analysis demonstrated that only BRF2 overexpression was an independent prognostic factor for unfavorable overall survival (P = 0.021).

Conclusions: BRF2 is a promising biomarker to identify individuals with poor prognostic potential and a possible target for anti-angiogenic therapy for patients with early-stage NSCLC.

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Related in: MedlinePlus

(A): The expression pattern of BRF2 in lung cancer tissues. (a) High BRF2 expression in lung adenocarcinoma. (b): High BRF2 expression in lung squamous cell carcinoma tissues. (c): Negative BRF2 expression in lung adenocarcinoma. (d): Negative BRF2 expression in lung squamous cell carcinoma tissues. (e): Intratumoral microvessels were stained as brown by the anti-CD34 monoclonal antibody in lung cancer tissues (magnification×400). (B): Quantitative real-time PCR analyses of BRF2 mRNA in twelve pairs of matched NSCLC and noncancerous tissues with GAPDH as a loading control in both panels. (C): Protein levels of BRF2 expression were evaluated by western blotting from paired noncancerous tissue and NSCLC.
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pone-0088032-g001: (A): The expression pattern of BRF2 in lung cancer tissues. (a) High BRF2 expression in lung adenocarcinoma. (b): High BRF2 expression in lung squamous cell carcinoma tissues. (c): Negative BRF2 expression in lung adenocarcinoma. (d): Negative BRF2 expression in lung squamous cell carcinoma tissues. (e): Intratumoral microvessels were stained as brown by the anti-CD34 monoclonal antibody in lung cancer tissues (magnification×400). (B): Quantitative real-time PCR analyses of BRF2 mRNA in twelve pairs of matched NSCLC and noncancerous tissues with GAPDH as a loading control in both panels. (C): Protein levels of BRF2 expression were evaluated by western blotting from paired noncancerous tissue and NSCLC.

Mentions: We detected the expression of BRF2 protein in the normal lung tissues, adjacent non-tumor tissues and tumor tissues by immunohistochemistry. As shown in Fig. 1 A, diffuse nuclear staining of BRF2 protein at various intensities was observed in cancer cells, but BRF2 was barely detected in normal lung tissues. In addition, some staining was observed in the cytoplasm of cancer cells. However, we observed no statistically significant correlation between BRF2 protein expression and any clinicopathological features of NSCLC tissues (P>0.05, Table 1). The mean value of BRF2 expression in 107 NSCLC tissues was 55.14%, significantly higher than that in adjacent tissues and normal lung tissues (36.96%, and 34.38%, respectively, P = 0.034 Table 2).


TFIIB-related factor 2 over expression is a prognosis marker for early-stage non-small cell lung cancer correlated with tumor angiogenesis.

Lu M, Tian H, Yue W, Li L, Li S, Qi L, Hu W, Gao C, Si L - PLoS ONE (2014)

(A): The expression pattern of BRF2 in lung cancer tissues. (a) High BRF2 expression in lung adenocarcinoma. (b): High BRF2 expression in lung squamous cell carcinoma tissues. (c): Negative BRF2 expression in lung adenocarcinoma. (d): Negative BRF2 expression in lung squamous cell carcinoma tissues. (e): Intratumoral microvessels were stained as brown by the anti-CD34 monoclonal antibody in lung cancer tissues (magnification×400). (B): Quantitative real-time PCR analyses of BRF2 mRNA in twelve pairs of matched NSCLC and noncancerous tissues with GAPDH as a loading control in both panels. (C): Protein levels of BRF2 expression were evaluated by western blotting from paired noncancerous tissue and NSCLC.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921153&req=5

pone-0088032-g001: (A): The expression pattern of BRF2 in lung cancer tissues. (a) High BRF2 expression in lung adenocarcinoma. (b): High BRF2 expression in lung squamous cell carcinoma tissues. (c): Negative BRF2 expression in lung adenocarcinoma. (d): Negative BRF2 expression in lung squamous cell carcinoma tissues. (e): Intratumoral microvessels were stained as brown by the anti-CD34 monoclonal antibody in lung cancer tissues (magnification×400). (B): Quantitative real-time PCR analyses of BRF2 mRNA in twelve pairs of matched NSCLC and noncancerous tissues with GAPDH as a loading control in both panels. (C): Protein levels of BRF2 expression were evaluated by western blotting from paired noncancerous tissue and NSCLC.
Mentions: We detected the expression of BRF2 protein in the normal lung tissues, adjacent non-tumor tissues and tumor tissues by immunohistochemistry. As shown in Fig. 1 A, diffuse nuclear staining of BRF2 protein at various intensities was observed in cancer cells, but BRF2 was barely detected in normal lung tissues. In addition, some staining was observed in the cytoplasm of cancer cells. However, we observed no statistically significant correlation between BRF2 protein expression and any clinicopathological features of NSCLC tissues (P>0.05, Table 1). The mean value of BRF2 expression in 107 NSCLC tissues was 55.14%, significantly higher than that in adjacent tissues and normal lung tissues (36.96%, and 34.38%, respectively, P = 0.034 Table 2).

Bottom Line: Intratumoral microvessel density (MVD) was measured by counting CD-34 positive immunostained endothelial cells.In addition, univariate and multivariate analysis demonstrated that BRF2 protein over-expression and high MVD were significantly associated with tumor relapse.Although BRF2 overexpression and high MVD indicated poor 5-year overall survival (p = 0.004 and p = 0.019, respectively), multivariate analysis demonstrated that only BRF2 overexpression was an independent prognostic factor for unfavorable overall survival (P = 0.021).

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic Surgery, Qilu Hospital, Shandong University, Jinan, P. R. China.

ABSTRACT

Background: The aim of this study was to examine BRF2 expression in patients with non-small cell lung cancer (NSCLC) and explore the relationship of BRF2 protein with clinicopathologic factors, tumor angiogenesis and prognosis.

Methods: Both BRF2 protein and intratumoral microvessels were examined by immunohistochemical staining in 107 non-small cell lung cancer patients. Intratumoral microvessel density (MVD) was measured by counting CD-34 positive immunostained endothelial cells. Western blot and RT-PCR analyses were utilized to investigate the BRF2 expression status in tissues.

Results: A notably higher level of BRF2 expression was found in NSCLC tissues at protein levels. In addition, univariate and multivariate analysis demonstrated that BRF2 protein over-expression and high MVD were significantly associated with tumor relapse. Although BRF2 overexpression and high MVD indicated poor 5-year overall survival (p = 0.004 and p = 0.019, respectively), multivariate analysis demonstrated that only BRF2 overexpression was an independent prognostic factor for unfavorable overall survival (P = 0.021).

Conclusions: BRF2 is a promising biomarker to identify individuals with poor prognostic potential and a possible target for anti-angiogenic therapy for patients with early-stage NSCLC.

Show MeSH
Related in: MedlinePlus