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Tc17 cells in patients with uterine cervical cancer.

Zhang Y, Hou F, Liu X, Ma D, Zhang Y, Kong B, Cui B - PLoS ONE (2014)

Bottom Line: Besides, the increased level of Tc17 in UCC patients was associated with the status of pelvic lymph node metastases and increased microvessel density.Finally, significant correlations of infiltration between Tc17 cells and Th17 cells or Foxp3-expressing T cells were observed in UCC and CIN tissues.This study indicates that Tc17 cell infiltration in cervical cancers is associated with cancer progression accompanied by increased infiltrations of Th17 cells and regulatory T cells as well as promoted tumor vasculogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong, People's Republic of China ; Department of Obstetrics and Gynecology, People's Hospital, Weifang City, Shandong, People's Republic of China ; Gynecology Oncology Key Library of Shandong Province, Qilu Hospital, Shandong University, Jinan, Shandong, People's Republic of China.

ABSTRACT

Background: The existence of Tc17 cells was recently shown in several types of infectious and autoimmune diseases, but their distribution and functions in uterine cervical cancer (UCC) have not been fully elucidated.

Methods: The frequency of Tc17 cells in peripheral blood samples obtained from UCC patients, cervical intraepithelial neoplasia (CIN) patients and healthy controls was determined by flow cytometry. Besides, the prevalence of Tc17 cells and their relationships to Th17 cells and Foxp3-expressing T cells as well as microvessels in tissue samples of the patients were assessed by immunohistochemistry staining.

Results: Compared to controls, patients with UCC or CIN had a higher proportion of Tc17 cells in both peripheral blood and cervical tissues, but the level of Tc17 cells in UCC tissues was significantly higher than that in CIN tissues. Besides, the increased level of Tc17 in UCC patients was associated with the status of pelvic lymph node metastases and increased microvessel density. Finally, significant correlations of infiltration between Tc17 cells and Th17 cells or Foxp3-expressing T cells were observed in UCC and CIN tissues.

Conclusions: This study indicates that Tc17 cell infiltration in cervical cancers is associated with cancer progression accompanied by increased infiltrations of Th17 cells and regulatory T cells as well as promoted tumor vasculogenesis.

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The frequency of Tc17 cells by immunohistochemical staining.(a) Compared to healthy controls (n = 30), significantly increased numbers of Tc17 cells were found in both tissues of UCC patients (P = 0.0007, n = 46) and CIN patients (P = 0.026, n = 28). Significant difference was also found between CIN and UCC tissues (P = 0.0086). (b) In tumor region, those UCC patients with lymph node metastases (n = 30) were detected significantly statistical higher Tc17 cells frequency than those patients without lymph node metastases (P = 0.035, n = 16). *P<0.05, **P<0.01, ****P<0.001.
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pone-0086812-g004: The frequency of Tc17 cells by immunohistochemical staining.(a) Compared to healthy controls (n = 30), significantly increased numbers of Tc17 cells were found in both tissues of UCC patients (P = 0.0007, n = 46) and CIN patients (P = 0.026, n = 28). Significant difference was also found between CIN and UCC tissues (P = 0.0086). (b) In tumor region, those UCC patients with lymph node metastases (n = 30) were detected significantly statistical higher Tc17 cells frequency than those patients without lymph node metastases (P = 0.035, n = 16). *P<0.05, **P<0.01, ****P<0.001.

Mentions: We previously found that IL-17+ cells were enriched in human UCC, and most of the circulating IL-17+ cells were CD4+ cell population (Th17 cells) [8]. However, in tumor tissues, a more abundance of the cells were CD8+ T cells (Tc17 cells). To study the distribution of Tc17 cells on a local scale, we examined the prevalence of Tc17 cells in tumor tissues from 46 UCC patients and cervical tissues from 28 CIN patients using immunohistochemical double staining (Fig. 3). As shown in Fig. 4a, the level of Tc17 cells significantly increased in tissues of UCC patients (142.50±28.24 cells/HPF) and CIN patients (23.42±7.65 cells/HPF), when compared to that in tissues of healthy controls (5.52±2.25 cells/HPF; PUCC = 0.0007, PCIN = 0.026). A significant difference was also found between CIN and UCC patients (P = 0.0086). Again, the level of Tc17 cells in tissues of UCC patients was positively correlated with the status of lymph node metastases, since significantly higher level of Tc17 cells was observed in tissues from UCC patients with lymph node metastases (178.20±35.47 cells/HPF) compared to that in UCC patients without lymph node metastases (116.59±25.57 cells/HPF, P = 0.035) (Fig. 4b). No correlation was found between the level of Tc17 cells with the other clinical characteristics such as clinical stage, infiltration depth, vasoinvasion, tumor size and histological tumor type of UCC patients (P>0.05).


Tc17 cells in patients with uterine cervical cancer.

Zhang Y, Hou F, Liu X, Ma D, Zhang Y, Kong B, Cui B - PLoS ONE (2014)

The frequency of Tc17 cells by immunohistochemical staining.(a) Compared to healthy controls (n = 30), significantly increased numbers of Tc17 cells were found in both tissues of UCC patients (P = 0.0007, n = 46) and CIN patients (P = 0.026, n = 28). Significant difference was also found between CIN and UCC tissues (P = 0.0086). (b) In tumor region, those UCC patients with lymph node metastases (n = 30) were detected significantly statistical higher Tc17 cells frequency than those patients without lymph node metastases (P = 0.035, n = 16). *P<0.05, **P<0.01, ****P<0.001.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921122&req=5

pone-0086812-g004: The frequency of Tc17 cells by immunohistochemical staining.(a) Compared to healthy controls (n = 30), significantly increased numbers of Tc17 cells were found in both tissues of UCC patients (P = 0.0007, n = 46) and CIN patients (P = 0.026, n = 28). Significant difference was also found between CIN and UCC tissues (P = 0.0086). (b) In tumor region, those UCC patients with lymph node metastases (n = 30) were detected significantly statistical higher Tc17 cells frequency than those patients without lymph node metastases (P = 0.035, n = 16). *P<0.05, **P<0.01, ****P<0.001.
Mentions: We previously found that IL-17+ cells were enriched in human UCC, and most of the circulating IL-17+ cells were CD4+ cell population (Th17 cells) [8]. However, in tumor tissues, a more abundance of the cells were CD8+ T cells (Tc17 cells). To study the distribution of Tc17 cells on a local scale, we examined the prevalence of Tc17 cells in tumor tissues from 46 UCC patients and cervical tissues from 28 CIN patients using immunohistochemical double staining (Fig. 3). As shown in Fig. 4a, the level of Tc17 cells significantly increased in tissues of UCC patients (142.50±28.24 cells/HPF) and CIN patients (23.42±7.65 cells/HPF), when compared to that in tissues of healthy controls (5.52±2.25 cells/HPF; PUCC = 0.0007, PCIN = 0.026). A significant difference was also found between CIN and UCC patients (P = 0.0086). Again, the level of Tc17 cells in tissues of UCC patients was positively correlated with the status of lymph node metastases, since significantly higher level of Tc17 cells was observed in tissues from UCC patients with lymph node metastases (178.20±35.47 cells/HPF) compared to that in UCC patients without lymph node metastases (116.59±25.57 cells/HPF, P = 0.035) (Fig. 4b). No correlation was found between the level of Tc17 cells with the other clinical characteristics such as clinical stage, infiltration depth, vasoinvasion, tumor size and histological tumor type of UCC patients (P>0.05).

Bottom Line: Besides, the increased level of Tc17 in UCC patients was associated with the status of pelvic lymph node metastases and increased microvessel density.Finally, significant correlations of infiltration between Tc17 cells and Th17 cells or Foxp3-expressing T cells were observed in UCC and CIN tissues.This study indicates that Tc17 cell infiltration in cervical cancers is associated with cancer progression accompanied by increased infiltrations of Th17 cells and regulatory T cells as well as promoted tumor vasculogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong, People's Republic of China ; Department of Obstetrics and Gynecology, People's Hospital, Weifang City, Shandong, People's Republic of China ; Gynecology Oncology Key Library of Shandong Province, Qilu Hospital, Shandong University, Jinan, Shandong, People's Republic of China.

ABSTRACT

Background: The existence of Tc17 cells was recently shown in several types of infectious and autoimmune diseases, but their distribution and functions in uterine cervical cancer (UCC) have not been fully elucidated.

Methods: The frequency of Tc17 cells in peripheral blood samples obtained from UCC patients, cervical intraepithelial neoplasia (CIN) patients and healthy controls was determined by flow cytometry. Besides, the prevalence of Tc17 cells and their relationships to Th17 cells and Foxp3-expressing T cells as well as microvessels in tissue samples of the patients were assessed by immunohistochemistry staining.

Results: Compared to controls, patients with UCC or CIN had a higher proportion of Tc17 cells in both peripheral blood and cervical tissues, but the level of Tc17 cells in UCC tissues was significantly higher than that in CIN tissues. Besides, the increased level of Tc17 in UCC patients was associated with the status of pelvic lymph node metastases and increased microvessel density. Finally, significant correlations of infiltration between Tc17 cells and Th17 cells or Foxp3-expressing T cells were observed in UCC and CIN tissues.

Conclusions: This study indicates that Tc17 cell infiltration in cervical cancers is associated with cancer progression accompanied by increased infiltrations of Th17 cells and regulatory T cells as well as promoted tumor vasculogenesis.

Show MeSH
Related in: MedlinePlus