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Tc17 cells in patients with uterine cervical cancer.

Zhang Y, Hou F, Liu X, Ma D, Zhang Y, Kong B, Cui B - PLoS ONE (2014)

Bottom Line: Besides, the increased level of Tc17 in UCC patients was associated with the status of pelvic lymph node metastases and increased microvessel density.Finally, significant correlations of infiltration between Tc17 cells and Th17 cells or Foxp3-expressing T cells were observed in UCC and CIN tissues.This study indicates that Tc17 cell infiltration in cervical cancers is associated with cancer progression accompanied by increased infiltrations of Th17 cells and regulatory T cells as well as promoted tumor vasculogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong, People's Republic of China ; Department of Obstetrics and Gynecology, People's Hospital, Weifang City, Shandong, People's Republic of China ; Gynecology Oncology Key Library of Shandong Province, Qilu Hospital, Shandong University, Jinan, Shandong, People's Republic of China.

ABSTRACT

Background: The existence of Tc17 cells was recently shown in several types of infectious and autoimmune diseases, but their distribution and functions in uterine cervical cancer (UCC) have not been fully elucidated.

Methods: The frequency of Tc17 cells in peripheral blood samples obtained from UCC patients, cervical intraepithelial neoplasia (CIN) patients and healthy controls was determined by flow cytometry. Besides, the prevalence of Tc17 cells and their relationships to Th17 cells and Foxp3-expressing T cells as well as microvessels in tissue samples of the patients were assessed by immunohistochemistry staining.

Results: Compared to controls, patients with UCC or CIN had a higher proportion of Tc17 cells in both peripheral blood and cervical tissues, but the level of Tc17 cells in UCC tissues was significantly higher than that in CIN tissues. Besides, the increased level of Tc17 in UCC patients was associated with the status of pelvic lymph node metastases and increased microvessel density. Finally, significant correlations of infiltration between Tc17 cells and Th17 cells or Foxp3-expressing T cells were observed in UCC and CIN tissues.

Conclusions: This study indicates that Tc17 cell infiltration in cervical cancers is associated with cancer progression accompanied by increased infiltrations of Th17 cells and regulatory T cells as well as promoted tumor vasculogenesis.

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Related in: MedlinePlus

The frequency of Tc17 cells by flow cytometry.(a) Tc17 frequencies in the three groups. Significantly increased Tc17 cells were found in untreated UCC patients (P = 0.0076, n = 49) and CIN patients (P = 0.0149, n = 25) compared to healthy controls (n = 28). (b) Tc17 frequency in positive or negative lymph node metastases in UCC patients. Compared with patients without lymph node metastases (n = 15), significantly increased Tc17 frequency (P = 0.0026) was found in lymph node metastases patients (n = 35). Student's t test was used and bars represent SD, *P<0.05, **P<0.01.
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pone-0086812-g002: The frequency of Tc17 cells by flow cytometry.(a) Tc17 frequencies in the three groups. Significantly increased Tc17 cells were found in untreated UCC patients (P = 0.0076, n = 49) and CIN patients (P = 0.0149, n = 25) compared to healthy controls (n = 28). (b) Tc17 frequency in positive or negative lymph node metastases in UCC patients. Compared with patients without lymph node metastases (n = 15), significantly increased Tc17 frequency (P = 0.0026) was found in lymph node metastases patients (n = 35). Student's t test was used and bars represent SD, *P<0.05, **P<0.01.

Mentions: We analyzed the expression of IL-17 on CD8+ T cells (Tc17 cells) in peripheral blood using flow cytometry. The expression of typical Tc17 cells in representative patients and controls was shown in Fig. 1. Besides, the percentage of Tc17 cells was significantly higher in UCC patients (0.49±0.37%, P = 0.0076) and CIN patients (0.51±0.39%, P = 0.0149), compared to that in healthy controls (0.27±0.27%) (Fig. 2a). No significant difference was found between the level of Tc17 cells in CIN and that in UCC patients (P>0.05). To investigate the possible role of the increased Tc17 cells, we next sought to determine whether this Tc17 skewing was associated with clinical features of the disease. Notably, we observed a significant increase in the frequency of Tc17 cells in UCC patients with lymph node metastasis (0.77±0.43%; P = 0.0026), when compared to that in patients without lymph node metastases (0.36±0.26%) (Fig. 2b). No association was found between the levels of Tc17 cells and other clinical characteristics such as clinical stage, infiltration depth, vasoinvasion, histological tumor type, or primary tumor size in UCC patients (P>0.05).


Tc17 cells in patients with uterine cervical cancer.

Zhang Y, Hou F, Liu X, Ma D, Zhang Y, Kong B, Cui B - PLoS ONE (2014)

The frequency of Tc17 cells by flow cytometry.(a) Tc17 frequencies in the three groups. Significantly increased Tc17 cells were found in untreated UCC patients (P = 0.0076, n = 49) and CIN patients (P = 0.0149, n = 25) compared to healthy controls (n = 28). (b) Tc17 frequency in positive or negative lymph node metastases in UCC patients. Compared with patients without lymph node metastases (n = 15), significantly increased Tc17 frequency (P = 0.0026) was found in lymph node metastases patients (n = 35). Student's t test was used and bars represent SD, *P<0.05, **P<0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921122&req=5

pone-0086812-g002: The frequency of Tc17 cells by flow cytometry.(a) Tc17 frequencies in the three groups. Significantly increased Tc17 cells were found in untreated UCC patients (P = 0.0076, n = 49) and CIN patients (P = 0.0149, n = 25) compared to healthy controls (n = 28). (b) Tc17 frequency in positive or negative lymph node metastases in UCC patients. Compared with patients without lymph node metastases (n = 15), significantly increased Tc17 frequency (P = 0.0026) was found in lymph node metastases patients (n = 35). Student's t test was used and bars represent SD, *P<0.05, **P<0.01.
Mentions: We analyzed the expression of IL-17 on CD8+ T cells (Tc17 cells) in peripheral blood using flow cytometry. The expression of typical Tc17 cells in representative patients and controls was shown in Fig. 1. Besides, the percentage of Tc17 cells was significantly higher in UCC patients (0.49±0.37%, P = 0.0076) and CIN patients (0.51±0.39%, P = 0.0149), compared to that in healthy controls (0.27±0.27%) (Fig. 2a). No significant difference was found between the level of Tc17 cells in CIN and that in UCC patients (P>0.05). To investigate the possible role of the increased Tc17 cells, we next sought to determine whether this Tc17 skewing was associated with clinical features of the disease. Notably, we observed a significant increase in the frequency of Tc17 cells in UCC patients with lymph node metastasis (0.77±0.43%; P = 0.0026), when compared to that in patients without lymph node metastases (0.36±0.26%) (Fig. 2b). No association was found between the levels of Tc17 cells and other clinical characteristics such as clinical stage, infiltration depth, vasoinvasion, histological tumor type, or primary tumor size in UCC patients (P>0.05).

Bottom Line: Besides, the increased level of Tc17 in UCC patients was associated with the status of pelvic lymph node metastases and increased microvessel density.Finally, significant correlations of infiltration between Tc17 cells and Th17 cells or Foxp3-expressing T cells were observed in UCC and CIN tissues.This study indicates that Tc17 cell infiltration in cervical cancers is associated with cancer progression accompanied by increased infiltrations of Th17 cells and regulatory T cells as well as promoted tumor vasculogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong, People's Republic of China ; Department of Obstetrics and Gynecology, People's Hospital, Weifang City, Shandong, People's Republic of China ; Gynecology Oncology Key Library of Shandong Province, Qilu Hospital, Shandong University, Jinan, Shandong, People's Republic of China.

ABSTRACT

Background: The existence of Tc17 cells was recently shown in several types of infectious and autoimmune diseases, but their distribution and functions in uterine cervical cancer (UCC) have not been fully elucidated.

Methods: The frequency of Tc17 cells in peripheral blood samples obtained from UCC patients, cervical intraepithelial neoplasia (CIN) patients and healthy controls was determined by flow cytometry. Besides, the prevalence of Tc17 cells and their relationships to Th17 cells and Foxp3-expressing T cells as well as microvessels in tissue samples of the patients were assessed by immunohistochemistry staining.

Results: Compared to controls, patients with UCC or CIN had a higher proportion of Tc17 cells in both peripheral blood and cervical tissues, but the level of Tc17 cells in UCC tissues was significantly higher than that in CIN tissues. Besides, the increased level of Tc17 in UCC patients was associated with the status of pelvic lymph node metastases and increased microvessel density. Finally, significant correlations of infiltration between Tc17 cells and Th17 cells or Foxp3-expressing T cells were observed in UCC and CIN tissues.

Conclusions: This study indicates that Tc17 cell infiltration in cervical cancers is associated with cancer progression accompanied by increased infiltrations of Th17 cells and regulatory T cells as well as promoted tumor vasculogenesis.

Show MeSH
Related in: MedlinePlus