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Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines.

Zhang J, Sun Q, Bo J, Huang R, Zhang M, Xia Z, Ju L, Xiang G - Int J Nanomedicine (2014)

Bottom Line: However, the interactions between the material itself and cancerous or normal cells have seldom been studied.To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied.Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT
Single-walled carbon nanohorns (SWNHs) may be useful as carriers for anticancer drugs due to their particular structure. However, the interactions between the material itself and cancerous or normal cells have seldom been studied. To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied. Our results showed that unmodified SWNHs inhibited mitotic entry, growth, and proliferation of human liver cell lines and promoted their apoptosis, especially in hepatoma cell lines. Individual spherical SWNH particles were found inside the nuclei of human hepatoma HepG2 cells and the lysosomes of normal human liver L02 cells, implying that SWNH particles could penetrate into human liver cells_and the different interacted mechanisms on human normal cell lines compared to hepatoma cell lines. Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

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SWNHs promoted cell apoptosis of liver cells, and apoptosis involves key factors in vivo.Notes: L02 cells l (3×105) and HepG2 cells (3×105) were seeded onto 60 mm noncoated and SWNH-coated dishes, respectively, and cultured for 48 hours. Then the effect of SWNHs on L02 (A) and HepG2 (B) cell apoptosis distribution was determined by flow cytometry. The expression levels of SIRT1 and those of activation cleavage of p53, caspase-3 and caspase-7 L02 (C) and HepG2 (D) were determined by Western Blotting. (A) The apoptotic L02 cells treated with SWNHs increased significantly at 48 hours after culture with SWNHs10 (P<0.05), and especially with SWNHs20, SWNHs30, and SWNHs40 (P<0.01) (B) The apoptotic HepG2 cells treated with SWNHs increased significantly at 48 hours after culture with SWNHs10 (P<0.05), and especially with SWNHs20, SWNHs30, and SWNHs40 (P<0.01). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; L02, normal liver cell line; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; SEM, standard error of the mean; SIRT1, silent information regulator 1.
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f6-ijn-9-759: SWNHs promoted cell apoptosis of liver cells, and apoptosis involves key factors in vivo.Notes: L02 cells l (3×105) and HepG2 cells (3×105) were seeded onto 60 mm noncoated and SWNH-coated dishes, respectively, and cultured for 48 hours. Then the effect of SWNHs on L02 (A) and HepG2 (B) cell apoptosis distribution was determined by flow cytometry. The expression levels of SIRT1 and those of activation cleavage of p53, caspase-3 and caspase-7 L02 (C) and HepG2 (D) were determined by Western Blotting. (A) The apoptotic L02 cells treated with SWNHs increased significantly at 48 hours after culture with SWNHs10 (P<0.05), and especially with SWNHs20, SWNHs30, and SWNHs40 (P<0.01) (B) The apoptotic HepG2 cells treated with SWNHs increased significantly at 48 hours after culture with SWNHs10 (P<0.05), and especially with SWNHs20, SWNHs30, and SWNHs40 (P<0.01). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; L02, normal liver cell line; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; SEM, standard error of the mean; SIRT1, silent information regulator 1.

Mentions: The cells were treated with SWNHs for 48 hours, and apoptotic cells were detected with Annexin-V. The results showed that apoptosis of L02 (Figure 6A) and HepG2 (Figure 6B) cells was improved in a SWNHs dose-dependent manner, especially for HepG2 cells (P<0.001).


Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines.

Zhang J, Sun Q, Bo J, Huang R, Zhang M, Xia Z, Ju L, Xiang G - Int J Nanomedicine (2014)

SWNHs promoted cell apoptosis of liver cells, and apoptosis involves key factors in vivo.Notes: L02 cells l (3×105) and HepG2 cells (3×105) were seeded onto 60 mm noncoated and SWNH-coated dishes, respectively, and cultured for 48 hours. Then the effect of SWNHs on L02 (A) and HepG2 (B) cell apoptosis distribution was determined by flow cytometry. The expression levels of SIRT1 and those of activation cleavage of p53, caspase-3 and caspase-7 L02 (C) and HepG2 (D) were determined by Western Blotting. (A) The apoptotic L02 cells treated with SWNHs increased significantly at 48 hours after culture with SWNHs10 (P<0.05), and especially with SWNHs20, SWNHs30, and SWNHs40 (P<0.01) (B) The apoptotic HepG2 cells treated with SWNHs increased significantly at 48 hours after culture with SWNHs10 (P<0.05), and especially with SWNHs20, SWNHs30, and SWNHs40 (P<0.01). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; L02, normal liver cell line; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; SEM, standard error of the mean; SIRT1, silent information regulator 1.
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Related In: Results  -  Collection

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Show All Figures
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f6-ijn-9-759: SWNHs promoted cell apoptosis of liver cells, and apoptosis involves key factors in vivo.Notes: L02 cells l (3×105) and HepG2 cells (3×105) were seeded onto 60 mm noncoated and SWNH-coated dishes, respectively, and cultured for 48 hours. Then the effect of SWNHs on L02 (A) and HepG2 (B) cell apoptosis distribution was determined by flow cytometry. The expression levels of SIRT1 and those of activation cleavage of p53, caspase-3 and caspase-7 L02 (C) and HepG2 (D) were determined by Western Blotting. (A) The apoptotic L02 cells treated with SWNHs increased significantly at 48 hours after culture with SWNHs10 (P<0.05), and especially with SWNHs20, SWNHs30, and SWNHs40 (P<0.01) (B) The apoptotic HepG2 cells treated with SWNHs increased significantly at 48 hours after culture with SWNHs10 (P<0.05), and especially with SWNHs20, SWNHs30, and SWNHs40 (P<0.01). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; L02, normal liver cell line; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; SEM, standard error of the mean; SIRT1, silent information regulator 1.
Mentions: The cells were treated with SWNHs for 48 hours, and apoptotic cells were detected with Annexin-V. The results showed that apoptosis of L02 (Figure 6A) and HepG2 (Figure 6B) cells was improved in a SWNHs dose-dependent manner, especially for HepG2 cells (P<0.001).

Bottom Line: However, the interactions between the material itself and cancerous or normal cells have seldom been studied.To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied.Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT
Single-walled carbon nanohorns (SWNHs) may be useful as carriers for anticancer drugs due to their particular structure. However, the interactions between the material itself and cancerous or normal cells have seldom been studied. To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied. Our results showed that unmodified SWNHs inhibited mitotic entry, growth, and proliferation of human liver cell lines and promoted their apoptosis, especially in hepatoma cell lines. Individual spherical SWNH particles were found inside the nuclei of human hepatoma HepG2 cells and the lysosomes of normal human liver L02 cells, implying that SWNH particles could penetrate into human liver cells_and the different interacted mechanisms on human normal cell lines compared to hepatoma cell lines. Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

Show MeSH
Related in: MedlinePlus