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Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines.

Zhang J, Sun Q, Bo J, Huang R, Zhang M, Xia Z, Ju L, Xiang G - Int J Nanomedicine (2014)

Bottom Line: However, the interactions between the material itself and cancerous or normal cells have seldom been studied.To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied.Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT
Single-walled carbon nanohorns (SWNHs) may be useful as carriers for anticancer drugs due to their particular structure. However, the interactions between the material itself and cancerous or normal cells have seldom been studied. To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied. Our results showed that unmodified SWNHs inhibited mitotic entry, growth, and proliferation of human liver cell lines and promoted their apoptosis, especially in hepatoma cell lines. Individual spherical SWNH particles were found inside the nuclei of human hepatoma HepG2 cells and the lysosomes of normal human liver L02 cells, implying that SWNH particles could penetrate into human liver cells_and the different interacted mechanisms on human normal cell lines compared to hepatoma cell lines. Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

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Related in: MedlinePlus

SWNHs affected cell cycle of liver cells.Notes: L02 cells (3×105) and HepG2 cells (3×105) were seeded onto noncoated and SWNH-coated 60 mm dishes, respectively, and cultured for 48 hours. Then the effects of SWNHs on L02 and HepG2 cell cycles were measured by flow cytometry. (A) G1 phase increased and S phase decreased significantly in an SWNH dose-dependent manner in L02 cells (P<0.05); G2 phase decreased in L02 cells. (B) G1 phase increased and S phase decreased significantly in an SWNH dose-dependent manner in HepG2 cells (P<0.01); G2 phase decreased in HepG2 cells, except it increased abruptly in HepG2 cells treated with SWNHs40 (P<0.05). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; L02, normal liver cell line; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; SEM, standard error of the mean.
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f5-ijn-9-759: SWNHs affected cell cycle of liver cells.Notes: L02 cells (3×105) and HepG2 cells (3×105) were seeded onto noncoated and SWNH-coated 60 mm dishes, respectively, and cultured for 48 hours. Then the effects of SWNHs on L02 and HepG2 cell cycles were measured by flow cytometry. (A) G1 phase increased and S phase decreased significantly in an SWNH dose-dependent manner in L02 cells (P<0.05); G2 phase decreased in L02 cells. (B) G1 phase increased and S phase decreased significantly in an SWNH dose-dependent manner in HepG2 cells (P<0.01); G2 phase decreased in HepG2 cells, except it increased abruptly in HepG2 cells treated with SWNHs40 (P<0.05). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; L02, normal liver cell line; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; SEM, standard error of the mean.

Mentions: The role on cell cycle by the material was measured with flow cytometry. The results showed that the effect of SWNHs on the cell cycles of L02 (Figure 5A) and HepG2 (Figure 5B) was dose-dependent.


Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines.

Zhang J, Sun Q, Bo J, Huang R, Zhang M, Xia Z, Ju L, Xiang G - Int J Nanomedicine (2014)

SWNHs affected cell cycle of liver cells.Notes: L02 cells (3×105) and HepG2 cells (3×105) were seeded onto noncoated and SWNH-coated 60 mm dishes, respectively, and cultured for 48 hours. Then the effects of SWNHs on L02 and HepG2 cell cycles were measured by flow cytometry. (A) G1 phase increased and S phase decreased significantly in an SWNH dose-dependent manner in L02 cells (P<0.05); G2 phase decreased in L02 cells. (B) G1 phase increased and S phase decreased significantly in an SWNH dose-dependent manner in HepG2 cells (P<0.01); G2 phase decreased in HepG2 cells, except it increased abruptly in HepG2 cells treated with SWNHs40 (P<0.05). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; L02, normal liver cell line; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; SEM, standard error of the mean.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3921087&req=5

f5-ijn-9-759: SWNHs affected cell cycle of liver cells.Notes: L02 cells (3×105) and HepG2 cells (3×105) were seeded onto noncoated and SWNH-coated 60 mm dishes, respectively, and cultured for 48 hours. Then the effects of SWNHs on L02 and HepG2 cell cycles were measured by flow cytometry. (A) G1 phase increased and S phase decreased significantly in an SWNH dose-dependent manner in L02 cells (P<0.05); G2 phase decreased in L02 cells. (B) G1 phase increased and S phase decreased significantly in an SWNH dose-dependent manner in HepG2 cells (P<0.01); G2 phase decreased in HepG2 cells, except it increased abruptly in HepG2 cells treated with SWNHs40 (P<0.05). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; L02, normal liver cell line; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; SEM, standard error of the mean.
Mentions: The role on cell cycle by the material was measured with flow cytometry. The results showed that the effect of SWNHs on the cell cycles of L02 (Figure 5A) and HepG2 (Figure 5B) was dose-dependent.

Bottom Line: However, the interactions between the material itself and cancerous or normal cells have seldom been studied.To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied.Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT
Single-walled carbon nanohorns (SWNHs) may be useful as carriers for anticancer drugs due to their particular structure. However, the interactions between the material itself and cancerous or normal cells have seldom been studied. To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied. Our results showed that unmodified SWNHs inhibited mitotic entry, growth, and proliferation of human liver cell lines and promoted their apoptosis, especially in hepatoma cell lines. Individual spherical SWNH particles were found inside the nuclei of human hepatoma HepG2 cells and the lysosomes of normal human liver L02 cells, implying that SWNH particles could penetrate into human liver cells_and the different interacted mechanisms on human normal cell lines compared to hepatoma cell lines. Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

Show MeSH
Related in: MedlinePlus