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Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines.

Zhang J, Sun Q, Bo J, Huang R, Zhang M, Xia Z, Ju L, Xiang G - Int J Nanomedicine (2014)

Bottom Line: However, the interactions between the material itself and cancerous or normal cells have seldom been studied.To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied.Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT
Single-walled carbon nanohorns (SWNHs) may be useful as carriers for anticancer drugs due to their particular structure. However, the interactions between the material itself and cancerous or normal cells have seldom been studied. To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied. Our results showed that unmodified SWNHs inhibited mitotic entry, growth, and proliferation of human liver cell lines and promoted their apoptosis, especially in hepatoma cell lines. Individual spherical SWNH particles were found inside the nuclei of human hepatoma HepG2 cells and the lysosomes of normal human liver L02 cells, implying that SWNH particles could penetrate into human liver cells_and the different interacted mechanisms on human normal cell lines compared to hepatoma cell lines. Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

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Related in: MedlinePlus

SWNHs inhibited growth and proliferation of liver cells.Notes: L02 cells (3×105) and HepG2 cells (3×105) were seeded onto 60 mm noncoated and SWNH-coated dishes, respectively, and cultured for 48 hours. Then the effects of SWNHs on the growth of L02 (A) and HepG2 (B) cells were investigated by XTT assays. L02 (Figure 4C, 3×103) and HepG2 (Figure 4D, 3×103) cells were cultured in 96-well plates (6 mm) treated with or without SWNHs, and the cell viabilities were evaluated by CCK-8 assay. (A) The XTT value of l02 cells treated with SWNHs decreased significantly from 36 hours after culture (P<0.05), especially after 48 hours (P<0.01). (B) The XTT value of HepG2 cells treated with SWNHs decreased significantly after 36 hours and 48 hours (P<0.01). (C) The absorbance of L02 cells decreased significantly from 24 hours and 36 hours after culture with SWNHs, and especially after 48 hours (P<0.01). (D) The absorbance of HepG2 cells decreased significantly from 24 hours after culture with SWNHs (P<0.05), and especially after 36 hours and 48 hours (P<0.01). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; XTT, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide; L02, normal liver cell line; BrdU, bromodeoxyuridine; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; CCK-8, Cell Counting Kit-8; SEM, standard error of the mean.
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f4-ijn-9-759: SWNHs inhibited growth and proliferation of liver cells.Notes: L02 cells (3×105) and HepG2 cells (3×105) were seeded onto 60 mm noncoated and SWNH-coated dishes, respectively, and cultured for 48 hours. Then the effects of SWNHs on the growth of L02 (A) and HepG2 (B) cells were investigated by XTT assays. L02 (Figure 4C, 3×103) and HepG2 (Figure 4D, 3×103) cells were cultured in 96-well plates (6 mm) treated with or without SWNHs, and the cell viabilities were evaluated by CCK-8 assay. (A) The XTT value of l02 cells treated with SWNHs decreased significantly from 36 hours after culture (P<0.05), especially after 48 hours (P<0.01). (B) The XTT value of HepG2 cells treated with SWNHs decreased significantly after 36 hours and 48 hours (P<0.01). (C) The absorbance of L02 cells decreased significantly from 24 hours and 36 hours after culture with SWNHs, and especially after 48 hours (P<0.01). (D) The absorbance of HepG2 cells decreased significantly from 24 hours after culture with SWNHs (P<0.05), and especially after 36 hours and 48 hours (P<0.01). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; XTT, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide; L02, normal liver cell line; BrdU, bromodeoxyuridine; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; CCK-8, Cell Counting Kit-8; SEM, standard error of the mean.

Mentions: The effect on cell growth by SWNHs was studied using XTT assay. The results showed that both L02 cells (Figure 4A) and HepG2 cells (Figure 4B) were significantly inhibited in a time- and dose-dependent manner (P<0.001). The results of cell viability assayed with CCK-8 also indicated that SWNHs inhibited cell proliferation of L02 cells (Figure 4C) and HepG2 cells (Figure 4D) and was time- and dose-dependent.


Single-walled carbon nanohorn (SWNH) aggregates inhibited proliferation of human liver cell lines and promoted apoptosis, especially for hepatoma cell lines.

Zhang J, Sun Q, Bo J, Huang R, Zhang M, Xia Z, Ju L, Xiang G - Int J Nanomedicine (2014)

SWNHs inhibited growth and proliferation of liver cells.Notes: L02 cells (3×105) and HepG2 cells (3×105) were seeded onto 60 mm noncoated and SWNH-coated dishes, respectively, and cultured for 48 hours. Then the effects of SWNHs on the growth of L02 (A) and HepG2 (B) cells were investigated by XTT assays. L02 (Figure 4C, 3×103) and HepG2 (Figure 4D, 3×103) cells were cultured in 96-well plates (6 mm) treated with or without SWNHs, and the cell viabilities were evaluated by CCK-8 assay. (A) The XTT value of l02 cells treated with SWNHs decreased significantly from 36 hours after culture (P<0.05), especially after 48 hours (P<0.01). (B) The XTT value of HepG2 cells treated with SWNHs decreased significantly after 36 hours and 48 hours (P<0.01). (C) The absorbance of L02 cells decreased significantly from 24 hours and 36 hours after culture with SWNHs, and especially after 48 hours (P<0.01). (D) The absorbance of HepG2 cells decreased significantly from 24 hours after culture with SWNHs (P<0.05), and especially after 36 hours and 48 hours (P<0.01). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; XTT, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide; L02, normal liver cell line; BrdU, bromodeoxyuridine; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; CCK-8, Cell Counting Kit-8; SEM, standard error of the mean.
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Related In: Results  -  Collection

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Show All Figures
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f4-ijn-9-759: SWNHs inhibited growth and proliferation of liver cells.Notes: L02 cells (3×105) and HepG2 cells (3×105) were seeded onto 60 mm noncoated and SWNH-coated dishes, respectively, and cultured for 48 hours. Then the effects of SWNHs on the growth of L02 (A) and HepG2 (B) cells were investigated by XTT assays. L02 (Figure 4C, 3×103) and HepG2 (Figure 4D, 3×103) cells were cultured in 96-well plates (6 mm) treated with or without SWNHs, and the cell viabilities were evaluated by CCK-8 assay. (A) The XTT value of l02 cells treated with SWNHs decreased significantly from 36 hours after culture (P<0.05), especially after 48 hours (P<0.01). (B) The XTT value of HepG2 cells treated with SWNHs decreased significantly after 36 hours and 48 hours (P<0.01). (C) The absorbance of L02 cells decreased significantly from 24 hours and 36 hours after culture with SWNHs, and especially after 48 hours (P<0.01). (D) The absorbance of HepG2 cells decreased significantly from 24 hours after culture with SWNHs (P<0.05), and especially after 36 hours and 48 hours (P<0.01). All data are represented as mean ± SEM. *P<0.05; **P<0.01.Abbreviations: SWNH, single-walled carbon nanohorn; XTT, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide; L02, normal liver cell line; BrdU, bromodeoxyuridine; SWNHs10, 0.21 μg of single-walled carbon nanohorns per cm2, or 6 μg per 60 mm dish; SWNHs20, 0.42 μg of single-walled carbon nanohorns per cm2, or 12 μg per 60 mm dish; SWNHs30, 0.64 μg of single-walled carbon nanohorns per cm2, or 18 μg per 60 mm dish; SWNHs40, 0.85 μg of single-walled carbon nanohorns per cm2, or 24 μg per 60 mm dish; HepG2, human hepatoma cell line; CCK-8, Cell Counting Kit-8; SEM, standard error of the mean.
Mentions: The effect on cell growth by SWNHs was studied using XTT assay. The results showed that both L02 cells (Figure 4A) and HepG2 cells (Figure 4B) were significantly inhibited in a time- and dose-dependent manner (P<0.001). The results of cell viability assayed with CCK-8 also indicated that SWNHs inhibited cell proliferation of L02 cells (Figure 4C) and HepG2 cells (Figure 4D) and was time- and dose-dependent.

Bottom Line: However, the interactions between the material itself and cancerous or normal cells have seldom been studied.To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied.Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, People's Republic of China.

ABSTRACT
Single-walled carbon nanohorns (SWNHs) may be useful as carriers for anticancer drugs due to their particular structure. However, the interactions between the material itself and cancerous or normal cells have seldom been studied. To address this problem, the effects of raw SWNH material on the biological functions of human liver cell lines were studied. Our results showed that unmodified SWNHs inhibited mitotic entry, growth, and proliferation of human liver cell lines and promoted their apoptosis, especially in hepatoma cell lines. Individual spherical SWNH particles were found inside the nuclei of human hepatoma HepG2 cells and the lysosomes of normal human liver L02 cells, implying that SWNH particles could penetrate into human liver cells_and the different interacted mechanisms on human normal cell lines compared to hepatoma cell lines. Further research on the mechanisms and application in treatment of hepatocellular carcinoma with SWNHs is needed.

Show MeSH
Related in: MedlinePlus