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Manual versus Automated Rodent Behavioral Assessment: Comparing Efficacy and Ease of Bederson and Garcia Neurological Deficit Scores to an Open Field Video-Tracking System.

Desland FA, Afzal A, Warraich Z, Mocco J - J Cent Nerv Syst Dis (2014)

Bottom Line: Results obtained from the manual Bederson and Garcia scales did not show significant differences between pre- and post-stroke animals in a small cohort.When using the same cohort, however, post-stroke data obtained from automated open field analysis showed significant differences in several parameters.These early data indicate use of automated open field analysis software may provide a more sensitive assessment when compared to traditional Bederson and Garcia scales.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurological Surgery, Vanderbilt University, Nashville, TN, USA.

ABSTRACT
Animal models of stroke have been crucial in advancing our understanding of the pathophysiology of cerebral ischemia. Currently, the standards for determining neurological deficit in rodents are the Bederson and Garcia scales, manual assessments scoring animals based on parameters ranked on a narrow scale of severity. Automated open field analysis of a live-video tracking system that analyzes animal behavior may provide a more sensitive test. Results obtained from the manual Bederson and Garcia scales did not show significant differences between pre- and post-stroke animals in a small cohort. When using the same cohort, however, post-stroke data obtained from automated open field analysis showed significant differences in several parameters. Furthermore, large cohort analysis also demonstrated increased sensitivity with automated open field analysis versus the Bederson and Garcia scales. These early data indicate use of automated open field analysis software may provide a more sensitive assessment when compared to traditional Bederson and Garcia scales.

No MeSH data available.


Related in: MedlinePlus

Automated Open Field Analysis Track Plots. Animals were placed in an open field and allowed to explore and move around in the cage. Animal behavior and movement patterns were recorded and analyzed using Ethovision XT software. The software tracked the nose (turquoise), center-point (red) and tail base (purple) of each animal. Visual representation of locomotor activity of a representative pre-stroke (A) and representative post-stroke (B) animal are shown.
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f2-jcnsd-6-2014-007: Automated Open Field Analysis Track Plots. Animals were placed in an open field and allowed to explore and move around in the cage. Animal behavior and movement patterns were recorded and analyzed using Ethovision XT software. The software tracked the nose (turquoise), center-point (red) and tail base (purple) of each animal. Visual representation of locomotor activity of a representative pre-stroke (A) and representative post-stroke (B) animal are shown.

Mentions: Figure 2 shows a representative track plot of an animal pre-stroke (Fig. 2A) and post-stroke (Fig. 2B). The turquoise path delineates the nose, red delineates the center of the animal and purple delineates the tail base of the animal. Qualitative visual inspection of the tracks created by the animals before and after stroke indicated that animals displayed a healthy exploratory behavior prior to the stroke in an open field, and a substantial decrease can be seen in the exploratory activity post-stroke, with activity that once comprised the entirety of the Phenotyper cage becoming limited to circling in the top-left and bottom-left corners of the arena. Closer inspection of the track plots also indicates the frequency of the nose spout at the feeder and water tray pre-stroke. Post stroke, however, the representative animal’s nose was not recorded in the feeder or the water zone.


Manual versus Automated Rodent Behavioral Assessment: Comparing Efficacy and Ease of Bederson and Garcia Neurological Deficit Scores to an Open Field Video-Tracking System.

Desland FA, Afzal A, Warraich Z, Mocco J - J Cent Nerv Syst Dis (2014)

Automated Open Field Analysis Track Plots. Animals were placed in an open field and allowed to explore and move around in the cage. Animal behavior and movement patterns were recorded and analyzed using Ethovision XT software. The software tracked the nose (turquoise), center-point (red) and tail base (purple) of each animal. Visual representation of locomotor activity of a representative pre-stroke (A) and representative post-stroke (B) animal are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3921024&req=5

f2-jcnsd-6-2014-007: Automated Open Field Analysis Track Plots. Animals were placed in an open field and allowed to explore and move around in the cage. Animal behavior and movement patterns were recorded and analyzed using Ethovision XT software. The software tracked the nose (turquoise), center-point (red) and tail base (purple) of each animal. Visual representation of locomotor activity of a representative pre-stroke (A) and representative post-stroke (B) animal are shown.
Mentions: Figure 2 shows a representative track plot of an animal pre-stroke (Fig. 2A) and post-stroke (Fig. 2B). The turquoise path delineates the nose, red delineates the center of the animal and purple delineates the tail base of the animal. Qualitative visual inspection of the tracks created by the animals before and after stroke indicated that animals displayed a healthy exploratory behavior prior to the stroke in an open field, and a substantial decrease can be seen in the exploratory activity post-stroke, with activity that once comprised the entirety of the Phenotyper cage becoming limited to circling in the top-left and bottom-left corners of the arena. Closer inspection of the track plots also indicates the frequency of the nose spout at the feeder and water tray pre-stroke. Post stroke, however, the representative animal’s nose was not recorded in the feeder or the water zone.

Bottom Line: Results obtained from the manual Bederson and Garcia scales did not show significant differences between pre- and post-stroke animals in a small cohort.When using the same cohort, however, post-stroke data obtained from automated open field analysis showed significant differences in several parameters.These early data indicate use of automated open field analysis software may provide a more sensitive assessment when compared to traditional Bederson and Garcia scales.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurological Surgery, Vanderbilt University, Nashville, TN, USA.

ABSTRACT
Animal models of stroke have been crucial in advancing our understanding of the pathophysiology of cerebral ischemia. Currently, the standards for determining neurological deficit in rodents are the Bederson and Garcia scales, manual assessments scoring animals based on parameters ranked on a narrow scale of severity. Automated open field analysis of a live-video tracking system that analyzes animal behavior may provide a more sensitive test. Results obtained from the manual Bederson and Garcia scales did not show significant differences between pre- and post-stroke animals in a small cohort. When using the same cohort, however, post-stroke data obtained from automated open field analysis showed significant differences in several parameters. Furthermore, large cohort analysis also demonstrated increased sensitivity with automated open field analysis versus the Bederson and Garcia scales. These early data indicate use of automated open field analysis software may provide a more sensitive assessment when compared to traditional Bederson and Garcia scales.

No MeSH data available.


Related in: MedlinePlus