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Antioxidant Potential of Crude Extract and Different Fractions of Enhydra fluctuans Lour.

Sannigrahi S, Kanti Mazuder U, Kumar Pal D, Parida S, Jain S - Iran J Pharm Res (2010)

Bottom Line: In addition, total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picryl hydrazyl) radical, nitric oxide, superoxide anion, hydroxyl radical scavenging activities and reductive power of crude extracted its different fractions were determined.It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (179.7 ± 18.23 μg / mg pyrocatechol equivalent).This fraction was found more effective than crude extract and other fractions in all the above mentioned assays.

View Article: PubMed Central - PubMed

Affiliation: St. Peter's Institute of Pharmaceutical Sciences, Warangal, Andhra Pradesh -506001, India.

ABSTRACT
The antioxidant potential of crude methanol extract (CE) as well as chloroform (CF), ethyl acetate (EF) and n-butanol (NF) soluble fractions of Enhydra fluctuans Lour, which is widely used in indigenous system of medicine for different purposes, were studied. The antioxidant potential of extract/different fractions were evaluated using different in vitro antioxidant models. In addition, total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picryl hydrazyl) radical, nitric oxide, superoxide anion, hydroxyl radical scavenging activities and reductive power of crude extracted its different fractions were determined. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (179.7 ± 18.23 μg / mg pyrocatechol equivalent). This fraction was found more effective than crude extract and other fractions in all the above mentioned assays.

No MeSH data available.


Related in: MedlinePlus

Scheme of extraction of aerial partsof Enhydra fluctuans Lour
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Figure 1: Scheme of extraction of aerial partsof Enhydra fluctuans Lour

Mentions: The plant material was air dried until dryness at room temperature and then powdered with a mill. Air-dried and powdered aerial part (1.8 kg) was extracted successively with petroleum ether (60-80°C) and methanol using Soxhlet apparatus. The solvents were then removed under reduced pressure to obtained sticky residues. The crude methanolic extract (105 g), after removal of the solvent, was dissolved in 10% sulfuric acid solution and partitioned with chloroform, ethyl acetate and n-buatnol successively to give chloroform (5.5 g), EtOAc (12.9 g), n-BuOH (17.5 g) and water soluble fractions (scheme 1).


Antioxidant Potential of Crude Extract and Different Fractions of Enhydra fluctuans Lour.

Sannigrahi S, Kanti Mazuder U, Kumar Pal D, Parida S, Jain S - Iran J Pharm Res (2010)

Scheme of extraction of aerial partsof Enhydra fluctuans Lour
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3869559&req=5

Figure 1: Scheme of extraction of aerial partsof Enhydra fluctuans Lour
Mentions: The plant material was air dried until dryness at room temperature and then powdered with a mill. Air-dried and powdered aerial part (1.8 kg) was extracted successively with petroleum ether (60-80°C) and methanol using Soxhlet apparatus. The solvents were then removed under reduced pressure to obtained sticky residues. The crude methanolic extract (105 g), after removal of the solvent, was dissolved in 10% sulfuric acid solution and partitioned with chloroform, ethyl acetate and n-buatnol successively to give chloroform (5.5 g), EtOAc (12.9 g), n-BuOH (17.5 g) and water soluble fractions (scheme 1).

Bottom Line: In addition, total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picryl hydrazyl) radical, nitric oxide, superoxide anion, hydroxyl radical scavenging activities and reductive power of crude extracted its different fractions were determined.It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (179.7 ± 18.23 μg / mg pyrocatechol equivalent).This fraction was found more effective than crude extract and other fractions in all the above mentioned assays.

View Article: PubMed Central - PubMed

Affiliation: St. Peter's Institute of Pharmaceutical Sciences, Warangal, Andhra Pradesh -506001, India.

ABSTRACT
The antioxidant potential of crude methanol extract (CE) as well as chloroform (CF), ethyl acetate (EF) and n-butanol (NF) soluble fractions of Enhydra fluctuans Lour, which is widely used in indigenous system of medicine for different purposes, were studied. The antioxidant potential of extract/different fractions were evaluated using different in vitro antioxidant models. In addition, total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picryl hydrazyl) radical, nitric oxide, superoxide anion, hydroxyl radical scavenging activities and reductive power of crude extracted its different fractions were determined. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (179.7 ± 18.23 μg / mg pyrocatechol equivalent). This fraction was found more effective than crude extract and other fractions in all the above mentioned assays.

No MeSH data available.


Related in: MedlinePlus