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Effects of atorvastatin on nitrate tolerance in diabetic rats.

Imenshahidi M, Karimi G, Kazemzadeh E - Iran J Pharm Res (2010)

Bottom Line: Groups 5 received neither atorvastatin nor nitroglycerin.The relaxation response to nitroglycerin in diabetic and normal aorta were not significantly different.The results showed that 8 weeks treatment with atorvastatin prevents nitrate tolerance in diabetic and normal rats, but in nitrate tolerant animals, 3 days treatment with atorvastatin was not effective on protection against nitrate tolerance.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Statins have been reported to show preventive effect on nitrate tolerance in normal rats, but there are no reports on their effect in diabetic animals. In this study, diabetes was induced in male wistar rats by a single intraperitoneal injection of streptozotocin (45 mg/kg). Five groups of diabetic and five groups of normal rats were treated; groups 1 (of normal and diabetic rats) received atorvastatin (10 mg/kg/d p.o. for 8 weeks) and groups 2 received atorvastatin (10 mg/kg/d p.o. for last 3 days). Groups 3 and groups 4 were similar to groups 1 and 2 respectively, except that they received nitroglycerin (50 mg/kg/d, b.i.d. for last 3 days of the study). Groups 5 received neither atorvastatin nor nitroglycerin. After 8 weeks, relaxations to nitroglycerin (0.01 to 10 nM) and nitroprusside (0.01 to 10 nM) were determined on phenylephrine-preconstricted aortic rings. The relaxation response to nitroglycerin in diabetic and normal aorta were not significantly different. The results showed that 8 weeks treatment with atorvastatin prevents nitrate tolerance in diabetic and normal rats, but in nitrate tolerant animals, 3 days treatment with atorvastatin was not effective on protection against nitrate tolerance.

No MeSH data available.


Related in: MedlinePlus

Concentration-response curves to nitroglycerin (NTG) in the phenylephrine (1 μM) pre-contracted aortic rings isolated from diabeticl rats exposed to NTG for 3 days (■), diabetic rats treated with atorvastatin (10 mg/kg/d p.o.) for 8 weeks and exposed to NTG for 3 days (▲) and diabetic rats treated with atorvastatin (10 mg/kg p.o.) for 3 days and exposed to NTG for 3 days (▼). Data are mean ± SEM. A statistically significant difference was observed between diabetic (3 days NTG) rats comparing with diabetic rats (3 days NTG) treated with atorvastatin (10 mg/kg/d p.o.) for 3 days ***P < 0.001; **P < 0.01; *P < 0.05. There is not significant difference between diabetic (3 days NTG) rats comparing with diabetic rats (3 days NTG) treated with atorvastatin (10 mg/kg p.o.) for 8 weeks (P > 0.05).
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Figure 3: Concentration-response curves to nitroglycerin (NTG) in the phenylephrine (1 μM) pre-contracted aortic rings isolated from diabeticl rats exposed to NTG for 3 days (■), diabetic rats treated with atorvastatin (10 mg/kg/d p.o.) for 8 weeks and exposed to NTG for 3 days (▲) and diabetic rats treated with atorvastatin (10 mg/kg p.o.) for 3 days and exposed to NTG for 3 days (▼). Data are mean ± SEM. A statistically significant difference was observed between diabetic (3 days NTG) rats comparing with diabetic rats (3 days NTG) treated with atorvastatin (10 mg/kg/d p.o.) for 3 days ***P < 0.001; **P < 0.01; *P < 0.05. There is not significant difference between diabetic (3 days NTG) rats comparing with diabetic rats (3 days NTG) treated with atorvastatin (10 mg/kg p.o.) for 8 weeks (P > 0.05).

Mentions: Eight weeks administration of atorvastatin prevented nitrate tolerance induction in diabetic animals; but 3 days atorvastatin was not effective on (Figure 3).


Effects of atorvastatin on nitrate tolerance in diabetic rats.

Imenshahidi M, Karimi G, Kazemzadeh E - Iran J Pharm Res (2010)

Concentration-response curves to nitroglycerin (NTG) in the phenylephrine (1 μM) pre-contracted aortic rings isolated from diabeticl rats exposed to NTG for 3 days (■), diabetic rats treated with atorvastatin (10 mg/kg/d p.o.) for 8 weeks and exposed to NTG for 3 days (▲) and diabetic rats treated with atorvastatin (10 mg/kg p.o.) for 3 days and exposed to NTG for 3 days (▼). Data are mean ± SEM. A statistically significant difference was observed between diabetic (3 days NTG) rats comparing with diabetic rats (3 days NTG) treated with atorvastatin (10 mg/kg/d p.o.) for 3 days ***P < 0.001; **P < 0.01; *P < 0.05. There is not significant difference between diabetic (3 days NTG) rats comparing with diabetic rats (3 days NTG) treated with atorvastatin (10 mg/kg p.o.) for 8 weeks (P > 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3869549&req=5

Figure 3: Concentration-response curves to nitroglycerin (NTG) in the phenylephrine (1 μM) pre-contracted aortic rings isolated from diabeticl rats exposed to NTG for 3 days (■), diabetic rats treated with atorvastatin (10 mg/kg/d p.o.) for 8 weeks and exposed to NTG for 3 days (▲) and diabetic rats treated with atorvastatin (10 mg/kg p.o.) for 3 days and exposed to NTG for 3 days (▼). Data are mean ± SEM. A statistically significant difference was observed between diabetic (3 days NTG) rats comparing with diabetic rats (3 days NTG) treated with atorvastatin (10 mg/kg/d p.o.) for 3 days ***P < 0.001; **P < 0.01; *P < 0.05. There is not significant difference between diabetic (3 days NTG) rats comparing with diabetic rats (3 days NTG) treated with atorvastatin (10 mg/kg p.o.) for 8 weeks (P > 0.05).
Mentions: Eight weeks administration of atorvastatin prevented nitrate tolerance induction in diabetic animals; but 3 days atorvastatin was not effective on (Figure 3).

Bottom Line: Groups 5 received neither atorvastatin nor nitroglycerin.The relaxation response to nitroglycerin in diabetic and normal aorta were not significantly different.The results showed that 8 weeks treatment with atorvastatin prevents nitrate tolerance in diabetic and normal rats, but in nitrate tolerant animals, 3 days treatment with atorvastatin was not effective on protection against nitrate tolerance.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Statins have been reported to show preventive effect on nitrate tolerance in normal rats, but there are no reports on their effect in diabetic animals. In this study, diabetes was induced in male wistar rats by a single intraperitoneal injection of streptozotocin (45 mg/kg). Five groups of diabetic and five groups of normal rats were treated; groups 1 (of normal and diabetic rats) received atorvastatin (10 mg/kg/d p.o. for 8 weeks) and groups 2 received atorvastatin (10 mg/kg/d p.o. for last 3 days). Groups 3 and groups 4 were similar to groups 1 and 2 respectively, except that they received nitroglycerin (50 mg/kg/d, b.i.d. for last 3 days of the study). Groups 5 received neither atorvastatin nor nitroglycerin. After 8 weeks, relaxations to nitroglycerin (0.01 to 10 nM) and nitroprusside (0.01 to 10 nM) were determined on phenylephrine-preconstricted aortic rings. The relaxation response to nitroglycerin in diabetic and normal aorta were not significantly different. The results showed that 8 weeks treatment with atorvastatin prevents nitrate tolerance in diabetic and normal rats, but in nitrate tolerant animals, 3 days treatment with atorvastatin was not effective on protection against nitrate tolerance.

No MeSH data available.


Related in: MedlinePlus