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Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats.

Ye F, Chen XQ, Bao GS, Hua Y, Wang ZD, Bao YC - Exp Ther Med (2013)

Bottom Line: The IL-6 mRNA expression in the hippocampi of the rats was analyzed using semi-quantitative reverse transcription-polymerase chain reaction, and control and topiramate (TPM)-treated groups were compared.TPM was able to depress the seizures and decrease the IL-6 level in the kindled rats.In conclusion, IL-6 mRNA was upregulated in the hippocampi of epileptic rats, and IL-6 may have participated in the process of kindling.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Jinhua Municipal Central Hospital, Jinhua, Zhejiang 321000, P.R. China.

ABSTRACT
The objective of this study was to analyze the changes in expression and the possible functions of interleukin-6 (IL-6) in electrical kindling of the basolateral amygdala (BLA) in epileptic rats. Bipolar electrodes were implanted into the BLA of Sprague-Dawley rats, and the rats were then subjected to chronic electrical stimulation through the electrodes to induce kindling. The seizure characteristics and behavioral changes of the rats were observed, and electroencephalograms were recorded during and following kindling. The IL-6 mRNA expression in the hippocampi of the rats was analyzed using semi-quantitative reverse transcription-polymerase chain reaction, and control and topiramate (TPM)-treated groups were compared. The mean time-period required for kindling was 13.50±3.99 days, and the afterdischarge duration (ADD) measured between 21,450 and 119,720 msec. The expression of IL-6 mRNA was significantly upregulated in the kindled rats. TPM was able to depress the seizures and decrease the IL-6 level in the kindled rats. In conclusion, IL-6 mRNA was upregulated in the hippocampi of epileptic rats, and IL-6 may have participated in the process of kindling.

No MeSH data available.


Related in: MedlinePlus

Electrophoresis of the reverse transcription polymerase chain reaction products of interleukin-6 (IL-6). Lane 1, blank control; lane 2, surgical control; lane 3, epilepsy; lane 4, topiramate (TPM); lane M: marker.
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f2-etm-07-01-0223: Electrophoresis of the reverse transcription polymerase chain reaction products of interleukin-6 (IL-6). Lane 1, blank control; lane 2, surgical control; lane 3, epilepsy; lane 4, topiramate (TPM); lane M: marker.

Mentions: The variance analyses of the semiquantitative RT-PCR results for IL-6 mRNA in the hippocampi of the rats in the four treatment groups were significantly different (F=5.959, P<0.05). As shown in Fig. 2, compared with the levels in the two control groups (1.57±0.69 for the blank control group and 1.80±0.54 for the surgical control group), the IL-6 mRNA levels (mean 2.83±0.40) in the hippocampi of rats in the epilepsy group were significantly increased (P<0.05). In addition, the TPM group (2.18±0.56) had a different recovery rate; however, the difference in IL-6 expression was not significant between the TPM and epilepsy groups (P>0.05; Fig. 2).


Effect of topiramate on interleukin 6 expression in the hippocampus of amygdala-kindled epileptic rats.

Ye F, Chen XQ, Bao GS, Hua Y, Wang ZD, Bao YC - Exp Ther Med (2013)

Electrophoresis of the reverse transcription polymerase chain reaction products of interleukin-6 (IL-6). Lane 1, blank control; lane 2, surgical control; lane 3, epilepsy; lane 4, topiramate (TPM); lane M: marker.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860984&req=5

f2-etm-07-01-0223: Electrophoresis of the reverse transcription polymerase chain reaction products of interleukin-6 (IL-6). Lane 1, blank control; lane 2, surgical control; lane 3, epilepsy; lane 4, topiramate (TPM); lane M: marker.
Mentions: The variance analyses of the semiquantitative RT-PCR results for IL-6 mRNA in the hippocampi of the rats in the four treatment groups were significantly different (F=5.959, P<0.05). As shown in Fig. 2, compared with the levels in the two control groups (1.57±0.69 for the blank control group and 1.80±0.54 for the surgical control group), the IL-6 mRNA levels (mean 2.83±0.40) in the hippocampi of rats in the epilepsy group were significantly increased (P<0.05). In addition, the TPM group (2.18±0.56) had a different recovery rate; however, the difference in IL-6 expression was not significant between the TPM and epilepsy groups (P>0.05; Fig. 2).

Bottom Line: The IL-6 mRNA expression in the hippocampi of the rats was analyzed using semi-quantitative reverse transcription-polymerase chain reaction, and control and topiramate (TPM)-treated groups were compared.TPM was able to depress the seizures and decrease the IL-6 level in the kindled rats.In conclusion, IL-6 mRNA was upregulated in the hippocampi of epileptic rats, and IL-6 may have participated in the process of kindling.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Jinhua Municipal Central Hospital, Jinhua, Zhejiang 321000, P.R. China.

ABSTRACT
The objective of this study was to analyze the changes in expression and the possible functions of interleukin-6 (IL-6) in electrical kindling of the basolateral amygdala (BLA) in epileptic rats. Bipolar electrodes were implanted into the BLA of Sprague-Dawley rats, and the rats were then subjected to chronic electrical stimulation through the electrodes to induce kindling. The seizure characteristics and behavioral changes of the rats were observed, and electroencephalograms were recorded during and following kindling. The IL-6 mRNA expression in the hippocampi of the rats was analyzed using semi-quantitative reverse transcription-polymerase chain reaction, and control and topiramate (TPM)-treated groups were compared. The mean time-period required for kindling was 13.50±3.99 days, and the afterdischarge duration (ADD) measured between 21,450 and 119,720 msec. The expression of IL-6 mRNA was significantly upregulated in the kindled rats. TPM was able to depress the seizures and decrease the IL-6 level in the kindled rats. In conclusion, IL-6 mRNA was upregulated in the hippocampi of epileptic rats, and IL-6 may have participated in the process of kindling.

No MeSH data available.


Related in: MedlinePlus