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Mechanisms of the immunosuppressive effects of mouse adipose tissue-derived mesenchymal stromal cells on mouse alloreactively stimulated spleen cells.

Nagaya R, Mizuno-Kamiya M, Takayama E, Kawaki H, Onoe I, Tanabe T, Nagahara K, Kondoh N - Exp Ther Med (2013)

Bottom Line: The suppressive effect of ASCs mediated by direct cell contact was partially reversed following knockdown of β2 microglobulin, a component of the major histocompatibility complex (MHC) class I molecule, with siRNA.The results of the study demonstrated that ASCs have significant immune modulatory effects on alloreactively stimulated spleen cells.The effects of ASCs on spleen cells are dependent on soluble factor(s) and cell contact, which is mediated by the MHC class I complex on ASCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Implantology, Asahi University School of Dentistry, Mizuho-shi, Gifu 501-0296, Japan.

ABSTRACT
The mechanisms of immunomodulation by mesenchymal stromal cells remain poorly understood. In this study, the effects of mouse adipose tissue-derived mesenchymal stromal cells (ASCs) on mouse spleen cells alloreactively stimulated by anti-CD3 and anti-CD28 antibody-coated (anti-CD3/CD28) beads were observed. Production of interferon-γ by the anti-CD3/CD28 bead-stimulated spleen cells was significantly suppressed in co-culture with ASCs. However, an augmented intensity of CD69 on the stimulated spleen cells was not suppressed in the presence of ASCs. The immunosuppressive effects of ASCs were partially mediated by one or more soluble factors (26% suppression). However, the ASCs require cell-cell contact in order to maximally exert suppression (88%). The suppressive effect of ASCs mediated by direct cell contact was partially reversed following knockdown of β2 microglobulin, a component of the major histocompatibility complex (MHC) class I molecule, with siRNA. The results of the study demonstrated that ASCs have significant immune modulatory effects on alloreactively stimulated spleen cells. The effects of ASCs on spleen cells are dependent on soluble factor(s) and cell contact, which is mediated by the MHC class I complex on ASCs.

No MeSH data available.


Related in: MedlinePlus

Differentiation of ASCs. ASCs were observed to differentiate toward (A) osteoblasts or (B) adipocytes in each inductive culture media following Alizarin red (A and C) and Oil Red O (B and D) staining. (C and D) No changes were observed in the cells that were not treated with inductive culture medium. ASCs, adipose tissue-derived mesenchymal stromal cells.
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f1-etm-07-01-0017: Differentiation of ASCs. ASCs were observed to differentiate toward (A) osteoblasts or (B) adipocytes in each inductive culture media following Alizarin red (A and C) and Oil Red O (B and D) staining. (C and D) No changes were observed in the cells that were not treated with inductive culture medium. ASCs, adipose tissue-derived mesenchymal stromal cells.

Mentions: ASCs were tested for their capacity to differentiate toward the osteogenic and adipogenic lineages. The cells treated with osteogenic medium underwent a morphological change demonstrating calcium deposition (Fig. 1A). In the adipogenic medium, the cells may have been induced toward adipogenic differentiation as shown by the accumulation of lipid vacuoles (Fig. 1B). However, no apparent changes were observed in untreated ASCs (Fig. 1C and D).


Mechanisms of the immunosuppressive effects of mouse adipose tissue-derived mesenchymal stromal cells on mouse alloreactively stimulated spleen cells.

Nagaya R, Mizuno-Kamiya M, Takayama E, Kawaki H, Onoe I, Tanabe T, Nagahara K, Kondoh N - Exp Ther Med (2013)

Differentiation of ASCs. ASCs were observed to differentiate toward (A) osteoblasts or (B) adipocytes in each inductive culture media following Alizarin red (A and C) and Oil Red O (B and D) staining. (C and D) No changes were observed in the cells that were not treated with inductive culture medium. ASCs, adipose tissue-derived mesenchymal stromal cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860983&req=5

f1-etm-07-01-0017: Differentiation of ASCs. ASCs were observed to differentiate toward (A) osteoblasts or (B) adipocytes in each inductive culture media following Alizarin red (A and C) and Oil Red O (B and D) staining. (C and D) No changes were observed in the cells that were not treated with inductive culture medium. ASCs, adipose tissue-derived mesenchymal stromal cells.
Mentions: ASCs were tested for their capacity to differentiate toward the osteogenic and adipogenic lineages. The cells treated with osteogenic medium underwent a morphological change demonstrating calcium deposition (Fig. 1A). In the adipogenic medium, the cells may have been induced toward adipogenic differentiation as shown by the accumulation of lipid vacuoles (Fig. 1B). However, no apparent changes were observed in untreated ASCs (Fig. 1C and D).

Bottom Line: The suppressive effect of ASCs mediated by direct cell contact was partially reversed following knockdown of β2 microglobulin, a component of the major histocompatibility complex (MHC) class I molecule, with siRNA.The results of the study demonstrated that ASCs have significant immune modulatory effects on alloreactively stimulated spleen cells.The effects of ASCs on spleen cells are dependent on soluble factor(s) and cell contact, which is mediated by the MHC class I complex on ASCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Implantology, Asahi University School of Dentistry, Mizuho-shi, Gifu 501-0296, Japan.

ABSTRACT
The mechanisms of immunomodulation by mesenchymal stromal cells remain poorly understood. In this study, the effects of mouse adipose tissue-derived mesenchymal stromal cells (ASCs) on mouse spleen cells alloreactively stimulated by anti-CD3 and anti-CD28 antibody-coated (anti-CD3/CD28) beads were observed. Production of interferon-γ by the anti-CD3/CD28 bead-stimulated spleen cells was significantly suppressed in co-culture with ASCs. However, an augmented intensity of CD69 on the stimulated spleen cells was not suppressed in the presence of ASCs. The immunosuppressive effects of ASCs were partially mediated by one or more soluble factors (26% suppression). However, the ASCs require cell-cell contact in order to maximally exert suppression (88%). The suppressive effect of ASCs mediated by direct cell contact was partially reversed following knockdown of β2 microglobulin, a component of the major histocompatibility complex (MHC) class I molecule, with siRNA. The results of the study demonstrated that ASCs have significant immune modulatory effects on alloreactively stimulated spleen cells. The effects of ASCs on spleen cells are dependent on soluble factor(s) and cell contact, which is mediated by the MHC class I complex on ASCs.

No MeSH data available.


Related in: MedlinePlus