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Effects of Porphyromonas gingivalis lipopolysaccharide on osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling.

Zhang Y, Wang XC, Bao XF, Hu M, Yu WX - Exp Ther Med (2013)

Bottom Line: In this study, the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling were investigated.Therefore, the results indicate that, when treated with Pg-LPS, the EphB4 receptor on osteoblasts and the EphrinB2 ligand on osteoclasts may generate bidirectional anti-osteoclastogenic and pro-osteoblastogenic signaling into respective cells and potentially facilitate the transition from bone resorption to bone formation.This study may contribute to the control of osteoblast differentiation and bone formation at remodeling, and possibly also modeling, sites.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.

ABSTRACT
In bone remodeling, the Eph family is involved in regulating the process of osteoclast and osteoblast coordination in order to maintain bone homeostasis. In this study, the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling were investigated. An osteoblast-osteoclast co-culture system was achieved successfully. Hence, direct contact and communication between osteoblasts and osteoclasts was permitted. Regarding the protein expression and gene expression of EphB4 and EphrinB2, it was shown that Pg-LPS increased the expression of EphB4 while inhibiting the expression of EphrinB2. Therefore, the results indicate that, when treated with Pg-LPS, the EphB4 receptor on osteoblasts and the EphrinB2 ligand on osteoclasts may generate bidirectional anti-osteoclastogenic and pro-osteoblastogenic signaling into respective cells and potentially facilitate the transition from bone resorption to bone formation. This study may contribute to the control of osteoblast differentiation and bone formation at remodeling, and possibly also modeling, sites.

No MeSH data available.


Images of (A) immunofluorescence staining and (B) western blot analysis. After being treated with Pg-LPS at a concentration of 75 ng/ml for 24 h, the expression of EphB4 in the osteoblast-osteoclast co-culture increased while that of EphrinB2 decreased. Pg-LPS, Porphyromonas gingivalis lipopolysaccharide.
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f4-etm-07-01-0080: Images of (A) immunofluorescence staining and (B) western blot analysis. After being treated with Pg-LPS at a concentration of 75 ng/ml for 24 h, the expression of EphB4 in the osteoblast-osteoclast co-culture increased while that of EphrinB2 decreased. Pg-LPS, Porphyromonas gingivalis lipopolysaccharide.

Mentions: As shown in Fig. 4, immunofluorescence staining and western blot analysis were conducted to study the changes in the expression levels of EphB4 and EphrinB2 proteins in the osteoblast-osteoblast co-culture. After being treated with Pg-LPS at a concentration of 75 ng/ml for 24 h, the expression of EphB4 increased, while that of EphrinB2 decreased.


Effects of Porphyromonas gingivalis lipopolysaccharide on osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling.

Zhang Y, Wang XC, Bao XF, Hu M, Yu WX - Exp Ther Med (2013)

Images of (A) immunofluorescence staining and (B) western blot analysis. After being treated with Pg-LPS at a concentration of 75 ng/ml for 24 h, the expression of EphB4 in the osteoblast-osteoclast co-culture increased while that of EphrinB2 decreased. Pg-LPS, Porphyromonas gingivalis lipopolysaccharide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860981&req=5

f4-etm-07-01-0080: Images of (A) immunofluorescence staining and (B) western blot analysis. After being treated with Pg-LPS at a concentration of 75 ng/ml for 24 h, the expression of EphB4 in the osteoblast-osteoclast co-culture increased while that of EphrinB2 decreased. Pg-LPS, Porphyromonas gingivalis lipopolysaccharide.
Mentions: As shown in Fig. 4, immunofluorescence staining and western blot analysis were conducted to study the changes in the expression levels of EphB4 and EphrinB2 proteins in the osteoblast-osteoblast co-culture. After being treated with Pg-LPS at a concentration of 75 ng/ml for 24 h, the expression of EphB4 increased, while that of EphrinB2 decreased.

Bottom Line: In this study, the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling were investigated.Therefore, the results indicate that, when treated with Pg-LPS, the EphB4 receptor on osteoblasts and the EphrinB2 ligand on osteoclasts may generate bidirectional anti-osteoclastogenic and pro-osteoblastogenic signaling into respective cells and potentially facilitate the transition from bone resorption to bone formation.This study may contribute to the control of osteoblast differentiation and bone formation at remodeling, and possibly also modeling, sites.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.

ABSTRACT
In bone remodeling, the Eph family is involved in regulating the process of osteoclast and osteoblast coordination in order to maintain bone homeostasis. In this study, the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling were investigated. An osteoblast-osteoclast co-culture system was achieved successfully. Hence, direct contact and communication between osteoblasts and osteoclasts was permitted. Regarding the protein expression and gene expression of EphB4 and EphrinB2, it was shown that Pg-LPS increased the expression of EphB4 while inhibiting the expression of EphrinB2. Therefore, the results indicate that, when treated with Pg-LPS, the EphB4 receptor on osteoblasts and the EphrinB2 ligand on osteoclasts may generate bidirectional anti-osteoclastogenic and pro-osteoblastogenic signaling into respective cells and potentially facilitate the transition from bone resorption to bone formation. This study may contribute to the control of osteoblast differentiation and bone formation at remodeling, and possibly also modeling, sites.

No MeSH data available.