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Effects of Porphyromonas gingivalis lipopolysaccharide on osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling.

Zhang Y, Wang XC, Bao XF, Hu M, Yu WX - Exp Ther Med (2013)

Bottom Line: In this study, the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling were investigated.Therefore, the results indicate that, when treated with Pg-LPS, the EphB4 receptor on osteoblasts and the EphrinB2 ligand on osteoclasts may generate bidirectional anti-osteoclastogenic and pro-osteoblastogenic signaling into respective cells and potentially facilitate the transition from bone resorption to bone formation.This study may contribute to the control of osteoblast differentiation and bone formation at remodeling, and possibly also modeling, sites.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.

ABSTRACT
In bone remodeling, the Eph family is involved in regulating the process of osteoclast and osteoblast coordination in order to maintain bone homeostasis. In this study, the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling were investigated. An osteoblast-osteoclast co-culture system was achieved successfully. Hence, direct contact and communication between osteoblasts and osteoclasts was permitted. Regarding the protein expression and gene expression of EphB4 and EphrinB2, it was shown that Pg-LPS increased the expression of EphB4 while inhibiting the expression of EphrinB2. Therefore, the results indicate that, when treated with Pg-LPS, the EphB4 receptor on osteoblasts and the EphrinB2 ligand on osteoclasts may generate bidirectional anti-osteoclastogenic and pro-osteoblastogenic signaling into respective cells and potentially facilitate the transition from bone resorption to bone formation. This study may contribute to the control of osteoblast differentiation and bone formation at remodeling, and possibly also modeling, sites.

No MeSH data available.


Comparison of EphrinB2 expression between the RAW 264.7 cells and the induced osteoclasts after four days, analyzed by (A) immunofluorescence staining and (B) western blot analysis. The images clearly show an increased expression of EphrinB2 in the osteoclasts and confirm the successful induction of osteoclasts.
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f2-etm-07-01-0080: Comparison of EphrinB2 expression between the RAW 264.7 cells and the induced osteoclasts after four days, analyzed by (A) immunofluorescence staining and (B) western blot analysis. The images clearly show an increased expression of EphrinB2 in the osteoclasts and confirm the successful induction of osteoclasts.

Mentions: The immunofluorescence staining (Fig. 2a) and western blot analysis (Fig. 2b) clearly show that the expression of EphrinB2 was higher in the induced osteoclasts than in the control cells.


Effects of Porphyromonas gingivalis lipopolysaccharide on osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling.

Zhang Y, Wang XC, Bao XF, Hu M, Yu WX - Exp Ther Med (2013)

Comparison of EphrinB2 expression between the RAW 264.7 cells and the induced osteoclasts after four days, analyzed by (A) immunofluorescence staining and (B) western blot analysis. The images clearly show an increased expression of EphrinB2 in the osteoclasts and confirm the successful induction of osteoclasts.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860981&req=5

f2-etm-07-01-0080: Comparison of EphrinB2 expression between the RAW 264.7 cells and the induced osteoclasts after four days, analyzed by (A) immunofluorescence staining and (B) western blot analysis. The images clearly show an increased expression of EphrinB2 in the osteoclasts and confirm the successful induction of osteoclasts.
Mentions: The immunofluorescence staining (Fig. 2a) and western blot analysis (Fig. 2b) clearly show that the expression of EphrinB2 was higher in the induced osteoclasts than in the control cells.

Bottom Line: In this study, the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling were investigated.Therefore, the results indicate that, when treated with Pg-LPS, the EphB4 receptor on osteoblasts and the EphrinB2 ligand on osteoclasts may generate bidirectional anti-osteoclastogenic and pro-osteoblastogenic signaling into respective cells and potentially facilitate the transition from bone resorption to bone formation.This study may contribute to the control of osteoblast differentiation and bone formation at remodeling, and possibly also modeling, sites.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthodontics, School of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.

ABSTRACT
In bone remodeling, the Eph family is involved in regulating the process of osteoclast and osteoblast coordination in order to maintain bone homeostasis. In this study, the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the osteoblast-osteoclast bidirectional EphB4-EphrinB2 signaling were investigated. An osteoblast-osteoclast co-culture system was achieved successfully. Hence, direct contact and communication between osteoblasts and osteoclasts was permitted. Regarding the protein expression and gene expression of EphB4 and EphrinB2, it was shown that Pg-LPS increased the expression of EphB4 while inhibiting the expression of EphrinB2. Therefore, the results indicate that, when treated with Pg-LPS, the EphB4 receptor on osteoblasts and the EphrinB2 ligand on osteoclasts may generate bidirectional anti-osteoclastogenic and pro-osteoblastogenic signaling into respective cells and potentially facilitate the transition from bone resorption to bone formation. This study may contribute to the control of osteoblast differentiation and bone formation at remodeling, and possibly also modeling, sites.

No MeSH data available.