Limits...
Correlation of fibrinogen-like protein 2 with disease progression in patients with severe acute pancreatitis.

Ye X, Huai J, Chen R, Ding J, Chen Y, Cai Z - Exp Ther Med (2013)

Bottom Line: The levels of hfgl2 expression in the PBMCs from the 25 patients with SAP were markedly upregulated compared with the other groups, whereas no significant difference between the MAP group and healthy controls was observed. hfgl2 expression in the PBMCs and pancreatic tissues was detectable through using immunohistochemistry and was demonstrated to be specifically localized to the endothelium of microvessels and inflammatory infiltrative cells in the areas of acute focal, confluent necrosis.There were positive correlations between hfgl2 expression in the PBMCs and the severity of SAP, as indicated by scores of Ranson and Acute Physiology and Chronic Health Evaluation II.The results suggest that hfgl2 is involved in the pathogenesis of SAP and hfgl2 levels may serve as a biomarker during disease progression.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Hepatology, Jinhua Municipal Central Hospital, Jinhua Hospital of Zhejiang University, Jinhua, Zhejiang 321000, P.R. China.

ABSTRACT
It has recently been demonstrated that fibrinogen-like protein 2 (fgl2) is expressed on the surface of macrophages, T cells and endothelial cells and directly cleaves prothrombin to thrombin. The present study was designed to examine fgl2 expression in patients with severe acute pancreatitis (SAP) and its correlation with disease progression. Peripheral blood mononuclear cells (PBMCs) were isolated from 25 patients with SAP, 37 patients with mild acute pancreatitis (MAP) and 20 healthy volunteers as controls. Paraffin sections of pancreas were obtained from 18 postoperative patients with SAP between 2003 and 2012. Human fgl2 (hfgl2) gene expression was determined in the PBMCs by real-time PCR. A monoclonal antibody against hfgl2 was applied to detect hfgl2 protein expression in the pancreatic tissues as well as in the PBMCs by immunohistochemical staining. The levels of hfgl2 expression in the PBMCs from the 25 patients with SAP were markedly upregulated compared with the other groups, whereas no significant difference between the MAP group and healthy controls was observed. hfgl2 expression in the PBMCs and pancreatic tissues was detectable through using immunohistochemistry and was demonstrated to be specifically localized to the endothelium of microvessels and inflammatory infiltrative cells in the areas of acute focal, confluent necrosis. There were positive correlations between hfgl2 expression in the PBMCs and the severity of SAP, as indicated by scores of Ranson and Acute Physiology and Chronic Health Evaluation II. The results suggest that hfgl2 is involved in the pathogenesis of SAP and hfgl2 levels may serve as a biomarker during disease progression.

No MeSH data available.


Related in: MedlinePlus

Hfgl2 expression in the pancreatic tissues of patients with (A) SAP and (B) MAP, identified by immunochemical staining (×200). Arrows indicate hfgl2-positive cells. Hfgl2, human fibrinogen-like protein 2; SAP, severe acute pancreatitis; MAP, mild acute pancreatitis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3860872&req=5

f2-etm-07-01-0085: Hfgl2 expression in the pancreatic tissues of patients with (A) SAP and (B) MAP, identified by immunochemical staining (×200). Arrows indicate hfgl2-positive cells. Hfgl2, human fibrinogen-like protein 2; SAP, severe acute pancreatitis; MAP, mild acute pancreatitis.

Mentions: Paraffin sections were reviewed and collected from postoperative patients with SAP or MAP to evaluate hfgl2 expression in pancreatic tissues. Results of immunohistochemistry demonstrated that hfgl2 was primarily localized in infiltrating interstitial and endothelial cells of the microvasculature, whereas little fgl2 was identified in the pancreatic tissues of the patients with MAP (Fig. 2).


Correlation of fibrinogen-like protein 2 with disease progression in patients with severe acute pancreatitis.

Ye X, Huai J, Chen R, Ding J, Chen Y, Cai Z - Exp Ther Med (2013)

Hfgl2 expression in the pancreatic tissues of patients with (A) SAP and (B) MAP, identified by immunochemical staining (×200). Arrows indicate hfgl2-positive cells. Hfgl2, human fibrinogen-like protein 2; SAP, severe acute pancreatitis; MAP, mild acute pancreatitis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860872&req=5

f2-etm-07-01-0085: Hfgl2 expression in the pancreatic tissues of patients with (A) SAP and (B) MAP, identified by immunochemical staining (×200). Arrows indicate hfgl2-positive cells. Hfgl2, human fibrinogen-like protein 2; SAP, severe acute pancreatitis; MAP, mild acute pancreatitis.
Mentions: Paraffin sections were reviewed and collected from postoperative patients with SAP or MAP to evaluate hfgl2 expression in pancreatic tissues. Results of immunohistochemistry demonstrated that hfgl2 was primarily localized in infiltrating interstitial and endothelial cells of the microvasculature, whereas little fgl2 was identified in the pancreatic tissues of the patients with MAP (Fig. 2).

Bottom Line: The levels of hfgl2 expression in the PBMCs from the 25 patients with SAP were markedly upregulated compared with the other groups, whereas no significant difference between the MAP group and healthy controls was observed. hfgl2 expression in the PBMCs and pancreatic tissues was detectable through using immunohistochemistry and was demonstrated to be specifically localized to the endothelium of microvessels and inflammatory infiltrative cells in the areas of acute focal, confluent necrosis.There were positive correlations between hfgl2 expression in the PBMCs and the severity of SAP, as indicated by scores of Ranson and Acute Physiology and Chronic Health Evaluation II.The results suggest that hfgl2 is involved in the pathogenesis of SAP and hfgl2 levels may serve as a biomarker during disease progression.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Hepatology, Jinhua Municipal Central Hospital, Jinhua Hospital of Zhejiang University, Jinhua, Zhejiang 321000, P.R. China.

ABSTRACT
It has recently been demonstrated that fibrinogen-like protein 2 (fgl2) is expressed on the surface of macrophages, T cells and endothelial cells and directly cleaves prothrombin to thrombin. The present study was designed to examine fgl2 expression in patients with severe acute pancreatitis (SAP) and its correlation with disease progression. Peripheral blood mononuclear cells (PBMCs) were isolated from 25 patients with SAP, 37 patients with mild acute pancreatitis (MAP) and 20 healthy volunteers as controls. Paraffin sections of pancreas were obtained from 18 postoperative patients with SAP between 2003 and 2012. Human fgl2 (hfgl2) gene expression was determined in the PBMCs by real-time PCR. A monoclonal antibody against hfgl2 was applied to detect hfgl2 protein expression in the pancreatic tissues as well as in the PBMCs by immunohistochemical staining. The levels of hfgl2 expression in the PBMCs from the 25 patients with SAP were markedly upregulated compared with the other groups, whereas no significant difference between the MAP group and healthy controls was observed. hfgl2 expression in the PBMCs and pancreatic tissues was detectable through using immunohistochemistry and was demonstrated to be specifically localized to the endothelium of microvessels and inflammatory infiltrative cells in the areas of acute focal, confluent necrosis. There were positive correlations between hfgl2 expression in the PBMCs and the severity of SAP, as indicated by scores of Ranson and Acute Physiology and Chronic Health Evaluation II. The results suggest that hfgl2 is involved in the pathogenesis of SAP and hfgl2 levels may serve as a biomarker during disease progression.

No MeSH data available.


Related in: MedlinePlus