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Laser-assisted microdissection.

Oberbeck D - Alcohol Res Health (2008)

Bottom Line: The challenge then becomes how to isolate small numbers of cells from a specific brain region without including unwanted cells.One approach to solving this problem is to use a technology known as laser-assisted microdissection (LMD).This article reviews some of the principles of LMD and its use in alcohol research.

View Article: PubMed Central - PubMed

Affiliation: Department of Behavioral Neuroscience, Oregon Health & Science University, and in the Research Service, Portland VA Medical Center, both in Portland, Oregon.

ABSTRACT
When analyzing alcohol's effects on the brain, researchers often want to look at small clusters of cells that can be studied in isolation from the surrounding brain tissue rather than at the entire brain or larger brain areas. This implies that relatively small numbers of cells have to be retrieved from the brain and studied in culture or subjected to biochemical analyses. The challenge then becomes how to isolate small numbers of cells from a specific brain region without including unwanted cells. One approach to solving this problem is to use a technology known as laser-assisted microdissection (LMD). This article reviews some of the principles of LMD and its use in alcohol research.

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Schematic showing laser capture microdissection.
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f7-arh-31-3-249: Schematic showing laser capture microdissection.

Mentions: Laser capture technology utilizes a low-powered infrared laser to activate an adhesive thermoplastic film on the cap of a microcentrifuge tube that has been placed over the cells of interest. Through this activation, the film directly “captures” the cells on the cap, and they can then be analyzed further (see figure 7).


Laser-assisted microdissection.

Oberbeck D - Alcohol Res Health (2008)

Schematic showing laser capture microdissection.
© Copyright Policy - public-domain
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860489&req=5

f7-arh-31-3-249: Schematic showing laser capture microdissection.
Mentions: Laser capture technology utilizes a low-powered infrared laser to activate an adhesive thermoplastic film on the cap of a microcentrifuge tube that has been placed over the cells of interest. Through this activation, the film directly “captures” the cells on the cap, and they can then be analyzed further (see figure 7).

Bottom Line: The challenge then becomes how to isolate small numbers of cells from a specific brain region without including unwanted cells.One approach to solving this problem is to use a technology known as laser-assisted microdissection (LMD).This article reviews some of the principles of LMD and its use in alcohol research.

View Article: PubMed Central - PubMed

Affiliation: Department of Behavioral Neuroscience, Oregon Health & Science University, and in the Research Service, Portland VA Medical Center, both in Portland, Oregon.

ABSTRACT
When analyzing alcohol's effects on the brain, researchers often want to look at small clusters of cells that can be studied in isolation from the surrounding brain tissue rather than at the entire brain or larger brain areas. This implies that relatively small numbers of cells have to be retrieved from the brain and studied in culture or subjected to biochemical analyses. The challenge then becomes how to isolate small numbers of cells from a specific brain region without including unwanted cells. One approach to solving this problem is to use a technology known as laser-assisted microdissection (LMD). This article reviews some of the principles of LMD and its use in alcohol research.

Show MeSH
Related in: MedlinePlus