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Effects of HLEC on the secreted proteins of epithelial ovarian cancer cells prone to metastasize to lymph nodes.

Zhang XY, Yin FQ, Liu L, Gao T, Ruan HY, Guan X, Lu YX, Li DR - Cancer Biol Med (2013)

Bottom Line: Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other.The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors.The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.

View Article: PubMed Central - PubMed

Affiliation: The Cancer Hospital Affiliated to Guangxi Medical University, Nanning 530021, China;

ABSTRACT

Objective: To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer (EOC) cells (SKOV3-PM4) with directional highly lymphatic metastasis.

Methods: Supernatants of four groups of cultured cells, namely, SKOV3 (A), SKOV3+HLEC (B), SKOV3-PM4 (C), SKOV3-PM4+HLEC (D), were collected, and their proteins were detected by antibody arrays and iTRAQ-2D-LC-MALDI-TOF/TOF/MS. Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA.

Results: Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C (compared with group A), whereas IGFBP7 and SPARC were downregulated in group D (compared with group C). Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other. Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers, benign tumors, and control groups. Two proteins were upegulated in the first group. VEGFA in the control group was downregulated. For IGFBP, upregulation in the control group and down-regulation in the first group were also observed.

Conclusion: The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors. The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.

No MeSH data available.


Related in: MedlinePlus

IGFBP7 concentrations in serum and cell supernatant.
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f4: IGFBP7 concentrations in serum and cell supernatant.

Mentions: Bioinformatic analysis results show that IGFBP7 is closely correlated with VEGFA protein, but no scientific reports on IGFBP7 and ovarian cancer are currently available. Therefore, IGFBP7 and VEGFA were selected for ELISA verification in this study. With regard to VEGFA, any pairwise comparison was statistically significant (P<0.05) among the three groups, in which the expression level in the malignant group was the highest. As for IGFBP7, the difference of any pairwise comparison was also statistically significant, in which the tendency was opposite to that of VEGFA, that is, the expression level in the normal group was the highest. In pairwise comparison of the cell supernatants between SKOV3 and SKOV3-PM4 as well as SKOV3-PM4 and SKOV3-PM4+HLEC, the expression tendencies of both proteins were statistically significant (P<0.05), which was similar to array detection and MS (Figures 3,4).


Effects of HLEC on the secreted proteins of epithelial ovarian cancer cells prone to metastasize to lymph nodes.

Zhang XY, Yin FQ, Liu L, Gao T, Ruan HY, Guan X, Lu YX, Li DR - Cancer Biol Med (2013)

IGFBP7 concentrations in serum and cell supernatant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860345&req=5

f4: IGFBP7 concentrations in serum and cell supernatant.
Mentions: Bioinformatic analysis results show that IGFBP7 is closely correlated with VEGFA protein, but no scientific reports on IGFBP7 and ovarian cancer are currently available. Therefore, IGFBP7 and VEGFA were selected for ELISA verification in this study. With regard to VEGFA, any pairwise comparison was statistically significant (P<0.05) among the three groups, in which the expression level in the malignant group was the highest. As for IGFBP7, the difference of any pairwise comparison was also statistically significant, in which the tendency was opposite to that of VEGFA, that is, the expression level in the normal group was the highest. In pairwise comparison of the cell supernatants between SKOV3 and SKOV3-PM4 as well as SKOV3-PM4 and SKOV3-PM4+HLEC, the expression tendencies of both proteins were statistically significant (P<0.05), which was similar to array detection and MS (Figures 3,4).

Bottom Line: Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other.The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors.The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.

View Article: PubMed Central - PubMed

Affiliation: The Cancer Hospital Affiliated to Guangxi Medical University, Nanning 530021, China;

ABSTRACT

Objective: To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer (EOC) cells (SKOV3-PM4) with directional highly lymphatic metastasis.

Methods: Supernatants of four groups of cultured cells, namely, SKOV3 (A), SKOV3+HLEC (B), SKOV3-PM4 (C), SKOV3-PM4+HLEC (D), were collected, and their proteins were detected by antibody arrays and iTRAQ-2D-LC-MALDI-TOF/TOF/MS. Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA.

Results: Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C (compared with group A), whereas IGFBP7 and SPARC were downregulated in group D (compared with group C). Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other. Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers, benign tumors, and control groups. Two proteins were upegulated in the first group. VEGFA in the control group was downregulated. For IGFBP, upregulation in the control group and down-regulation in the first group were also observed.

Conclusion: The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors. The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.

No MeSH data available.


Related in: MedlinePlus