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Effects of HLEC on the secreted proteins of epithelial ovarian cancer cells prone to metastasize to lymph nodes.

Zhang XY, Yin FQ, Liu L, Gao T, Ruan HY, Guan X, Lu YX, Li DR - Cancer Biol Med (2013)

Bottom Line: Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other.The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors.The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.

View Article: PubMed Central - PubMed

Affiliation: The Cancer Hospital Affiliated to Guangxi Medical University, Nanning 530021, China;

ABSTRACT

Objective: To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer (EOC) cells (SKOV3-PM4) with directional highly lymphatic metastasis.

Methods: Supernatants of four groups of cultured cells, namely, SKOV3 (A), SKOV3+HLEC (B), SKOV3-PM4 (C), SKOV3-PM4+HLEC (D), were collected, and their proteins were detected by antibody arrays and iTRAQ-2D-LC-MALDI-TOF/TOF/MS. Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA.

Results: Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C (compared with group A), whereas IGFBP7 and SPARC were downregulated in group D (compared with group C). Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other. Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers, benign tumors, and control groups. Two proteins were upegulated in the first group. VEGFA in the control group was downregulated. For IGFBP, upregulation in the control group and down-regulation in the first group were also observed.

Conclusion: The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors. The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.

No MeSH data available.


Related in: MedlinePlus

Function prediction of IGFBP7 by GeneMANIA.
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f2: Function prediction of IGFBP7 by GeneMANIA.

Mentions: Figure 1 shows different link thicknesses that represent different correlation degrees among proteins, biological processes, and EOC, as well as lymphatic metastasis. Thicker lines represent higher correlations between both ends of the line. The analysis showed that OEC is closely associated with VEGFA, SPARC, and GRN, but it is not correlated with IGFBP7. Interaction network among IGFBP7 and the three proteins that are closely correlated with ovarian cancer were drawn using GeneMania to analyze the relationship between IGFBP7 and lymphatic metastasis of ovarian cancer. In Figure 2, the lines in different colors represent different correlations, in which the thickness corresponds to the degree of correlation.


Effects of HLEC on the secreted proteins of epithelial ovarian cancer cells prone to metastasize to lymph nodes.

Zhang XY, Yin FQ, Liu L, Gao T, Ruan HY, Guan X, Lu YX, Li DR - Cancer Biol Med (2013)

Function prediction of IGFBP7 by GeneMANIA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860345&req=5

f2: Function prediction of IGFBP7 by GeneMANIA.
Mentions: Figure 1 shows different link thicknesses that represent different correlation degrees among proteins, biological processes, and EOC, as well as lymphatic metastasis. Thicker lines represent higher correlations between both ends of the line. The analysis showed that OEC is closely associated with VEGFA, SPARC, and GRN, but it is not correlated with IGFBP7. Interaction network among IGFBP7 and the three proteins that are closely correlated with ovarian cancer were drawn using GeneMania to analyze the relationship between IGFBP7 and lymphatic metastasis of ovarian cancer. In Figure 2, the lines in different colors represent different correlations, in which the thickness corresponds to the degree of correlation.

Bottom Line: Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other.The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors.The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.

View Article: PubMed Central - PubMed

Affiliation: The Cancer Hospital Affiliated to Guangxi Medical University, Nanning 530021, China;

ABSTRACT

Objective: To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer (EOC) cells (SKOV3-PM4) with directional highly lymphatic metastasis.

Methods: Supernatants of four groups of cultured cells, namely, SKOV3 (A), SKOV3+HLEC (B), SKOV3-PM4 (C), SKOV3-PM4+HLEC (D), were collected, and their proteins were detected by antibody arrays and iTRAQ-2D-LC-MALDI-TOF/TOF/MS. Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA.

Results: Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C (compared with group A), whereas IGFBP7 and SPARC were downregulated in group D (compared with group C). Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other. Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of patients with ovarian cancers, benign tumors, and control groups. Two proteins were upegulated in the first group. VEGFA in the control group was downregulated. For IGFBP, upregulation in the control group and down-regulation in the first group were also observed.

Conclusion: The HLEC microenvironment is closely associated with directional metastasis to lymph nodes and with differential proteins including cell stromal proteins and adhesion factors. The upregulation of VEGFA and GRN and the downregulation of SPARC and IGFBP7 are closely associated with directional metastasis to lymph nodes in EOC cells.

No MeSH data available.


Related in: MedlinePlus