Limits...
The Inhibitory Effect of 3 β -Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway.

Wang J, Chen X, Zhou Z, Li J, Sun H - Evid Based Complement Alternat Med (2013)

Bottom Line: 3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis.PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4.These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis. In this study, we investigated its effects on growth, apoptosis, cell cycle, motility/invasion, and metatasis in human hepatoma HepG2 cells in vitro and antimetastasis of B16-F10 melanoma in mice in vivo, as well as its molecular mechanisms of action using a high-throughput Cancer Pathway Finder PCR Array. ATA could not only induce tumor cells into apoptosis through the activation of both extrinsic and intrinsic pathways, arrest HepG2 cells in G2/M phase, but also suppress the invasion and metastasis abilities of HepG2 cells and the lung metastasis of B16-F10 melanoma in mice. PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4. These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

No MeSH data available.


Related in: MedlinePlus

RT-PCR validation of PCR array results. After cells were treated by 0, 10, and 20 μg/mL ATA for 16 h, expression changes of seven genes which were upregulated (p21, IL-8, TNF, and c-Jun) or downregulated (cyclinE1, AKT, and PLAU) in PCR array analysis detected by RT-PCR using specific primers as described in the text. The housekeeping gene β-actin was used as endogenous control. The figures shown were representative of three independent experiments. The values are presented as mean ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as **P < 0.01 and ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3860155&req=5

fig8: RT-PCR validation of PCR array results. After cells were treated by 0, 10, and 20 μg/mL ATA for 16 h, expression changes of seven genes which were upregulated (p21, IL-8, TNF, and c-Jun) or downregulated (cyclinE1, AKT, and PLAU) in PCR array analysis detected by RT-PCR using specific primers as described in the text. The housekeeping gene β-actin was used as endogenous control. The figures shown were representative of three independent experiments. The values are presented as mean ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as **P < 0.01 and ***P < 0.001.

Mentions: To confirm the validity of the PCR array results, semiquantitative RT-PCR was undertaken for 7 putative differentially expressed genes including 4 upregulated genes p21, IL-8, TNF, and c-Jun and 3 downregulated genes Cyclin E1, AKT, and PLAU. As shown in Figure 8, after HepG2 cells were treated with ATA, the expression levels of p21, IL-8, TNF, and c-Jun mRNA were increased, whereas those of Cyclin E1, AKT, and PLAU were significantly decreased in a concentration-dependent manner compared with the control (P < 0.01 and P < 0.001). Differential expression of selected genes was considered to be validated as the similar results between RT-PCR and PCR array data.


The Inhibitory Effect of 3 β -Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway.

Wang J, Chen X, Zhou Z, Li J, Sun H - Evid Based Complement Alternat Med (2013)

RT-PCR validation of PCR array results. After cells were treated by 0, 10, and 20 μg/mL ATA for 16 h, expression changes of seven genes which were upregulated (p21, IL-8, TNF, and c-Jun) or downregulated (cyclinE1, AKT, and PLAU) in PCR array analysis detected by RT-PCR using specific primers as described in the text. The housekeeping gene β-actin was used as endogenous control. The figures shown were representative of three independent experiments. The values are presented as mean ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as **P < 0.01 and ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3860155&req=5

fig8: RT-PCR validation of PCR array results. After cells were treated by 0, 10, and 20 μg/mL ATA for 16 h, expression changes of seven genes which were upregulated (p21, IL-8, TNF, and c-Jun) or downregulated (cyclinE1, AKT, and PLAU) in PCR array analysis detected by RT-PCR using specific primers as described in the text. The housekeeping gene β-actin was used as endogenous control. The figures shown were representative of three independent experiments. The values are presented as mean ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as **P < 0.01 and ***P < 0.001.
Mentions: To confirm the validity of the PCR array results, semiquantitative RT-PCR was undertaken for 7 putative differentially expressed genes including 4 upregulated genes p21, IL-8, TNF, and c-Jun and 3 downregulated genes Cyclin E1, AKT, and PLAU. As shown in Figure 8, after HepG2 cells were treated with ATA, the expression levels of p21, IL-8, TNF, and c-Jun mRNA were increased, whereas those of Cyclin E1, AKT, and PLAU were significantly decreased in a concentration-dependent manner compared with the control (P < 0.01 and P < 0.001). Differential expression of selected genes was considered to be validated as the similar results between RT-PCR and PCR array data.

Bottom Line: 3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis.PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4.These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis. In this study, we investigated its effects on growth, apoptosis, cell cycle, motility/invasion, and metatasis in human hepatoma HepG2 cells in vitro and antimetastasis of B16-F10 melanoma in mice in vivo, as well as its molecular mechanisms of action using a high-throughput Cancer Pathway Finder PCR Array. ATA could not only induce tumor cells into apoptosis through the activation of both extrinsic and intrinsic pathways, arrest HepG2 cells in G2/M phase, but also suppress the invasion and metastasis abilities of HepG2 cells and the lung metastasis of B16-F10 melanoma in mice. PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4. These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

No MeSH data available.


Related in: MedlinePlus