Limits...
The Inhibitory Effect of 3 β -Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway.

Wang J, Chen X, Zhou Z, Li J, Sun H - Evid Based Complement Alternat Med (2013)

Bottom Line: 3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis.PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4.These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis. In this study, we investigated its effects on growth, apoptosis, cell cycle, motility/invasion, and metatasis in human hepatoma HepG2 cells in vitro and antimetastasis of B16-F10 melanoma in mice in vivo, as well as its molecular mechanisms of action using a high-throughput Cancer Pathway Finder PCR Array. ATA could not only induce tumor cells into apoptosis through the activation of both extrinsic and intrinsic pathways, arrest HepG2 cells in G2/M phase, but also suppress the invasion and metastasis abilities of HepG2 cells and the lung metastasis of B16-F10 melanoma in mice. PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4. These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

No MeSH data available.


Related in: MedlinePlus

Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on the invasion of HepG2 cells. (a) HepG2 cells seeded in Transwell Boyden Chambers were treated by 0, 5, 10, and 20 μg/mL ATA for 16 h, and the cells invading through the matrigel were photographed after HE staining. (b) The invasion rate of HepG2 cells was expressed as the percentage of invading cells compared with the control. The values are presented as means ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3860155&req=5

fig7: Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on the invasion of HepG2 cells. (a) HepG2 cells seeded in Transwell Boyden Chambers were treated by 0, 5, 10, and 20 μg/mL ATA for 16 h, and the cells invading through the matrigel were photographed after HE staining. (b) The invasion rate of HepG2 cells was expressed as the percentage of invading cells compared with the control. The values are presented as means ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as ***P < 0.001.

Mentions: Transwell Boyden chamber assay was performed to determine the effect of ATA on the motility and invasive of HepG2 cells. As shown in Figure 7(a), incubation of control HepG2 cells in the chamber for 16 h resulted in large-scale migration of cancer cells to the lower side of the filter. In contrast, treatment with ATA caused a dose-dependent inhibition of HepG2 invasion. Compared to the control cell, the average migration rates of HepG2 cell treated ATA at the concentrations of 5, 10, and 20 μg/mL for 16 h were 74.3%, 64.9%, and 47.6%, respectively (Figure 7(b)). The result indicated that treatment with ATA induced a significant decrease in the invasiveness of HepG2 cells (P < 0.001).


The Inhibitory Effect of 3 β -Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway.

Wang J, Chen X, Zhou Z, Li J, Sun H - Evid Based Complement Alternat Med (2013)

Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on the invasion of HepG2 cells. (a) HepG2 cells seeded in Transwell Boyden Chambers were treated by 0, 5, 10, and 20 μg/mL ATA for 16 h, and the cells invading through the matrigel were photographed after HE staining. (b) The invasion rate of HepG2 cells was expressed as the percentage of invading cells compared with the control. The values are presented as means ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3860155&req=5

fig7: Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on the invasion of HepG2 cells. (a) HepG2 cells seeded in Transwell Boyden Chambers were treated by 0, 5, 10, and 20 μg/mL ATA for 16 h, and the cells invading through the matrigel were photographed after HE staining. (b) The invasion rate of HepG2 cells was expressed as the percentage of invading cells compared with the control. The values are presented as means ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as ***P < 0.001.
Mentions: Transwell Boyden chamber assay was performed to determine the effect of ATA on the motility and invasive of HepG2 cells. As shown in Figure 7(a), incubation of control HepG2 cells in the chamber for 16 h resulted in large-scale migration of cancer cells to the lower side of the filter. In contrast, treatment with ATA caused a dose-dependent inhibition of HepG2 invasion. Compared to the control cell, the average migration rates of HepG2 cell treated ATA at the concentrations of 5, 10, and 20 μg/mL for 16 h were 74.3%, 64.9%, and 47.6%, respectively (Figure 7(b)). The result indicated that treatment with ATA induced a significant decrease in the invasiveness of HepG2 cells (P < 0.001).

Bottom Line: 3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis.PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4.These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis. In this study, we investigated its effects on growth, apoptosis, cell cycle, motility/invasion, and metatasis in human hepatoma HepG2 cells in vitro and antimetastasis of B16-F10 melanoma in mice in vivo, as well as its molecular mechanisms of action using a high-throughput Cancer Pathway Finder PCR Array. ATA could not only induce tumor cells into apoptosis through the activation of both extrinsic and intrinsic pathways, arrest HepG2 cells in G2/M phase, but also suppress the invasion and metastasis abilities of HepG2 cells and the lung metastasis of B16-F10 melanoma in mice. PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4. These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

No MeSH data available.


Related in: MedlinePlus