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The Inhibitory Effect of 3 β -Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway.

Wang J, Chen X, Zhou Z, Li J, Sun H - Evid Based Complement Alternat Med (2013)

Bottom Line: 3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis.PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4.These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis. In this study, we investigated its effects on growth, apoptosis, cell cycle, motility/invasion, and metatasis in human hepatoma HepG2 cells in vitro and antimetastasis of B16-F10 melanoma in mice in vivo, as well as its molecular mechanisms of action using a high-throughput Cancer Pathway Finder PCR Array. ATA could not only induce tumor cells into apoptosis through the activation of both extrinsic and intrinsic pathways, arrest HepG2 cells in G2/M phase, but also suppress the invasion and metastasis abilities of HepG2 cells and the lung metastasis of B16-F10 melanoma in mice. PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4. These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

No MeSH data available.


Related in: MedlinePlus

Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on cell cycle distribution in HepG2 cells. After treatment with ATA at the concentrations of 0, 5, and 10 μg/mL for 48 h, the cells were collected and stained with propidium iodide (PI), with fluorescence intensities measured by flow cytometry. The histogram demonstrated the percentage of cells in various phase in HepG2 cells treated with ATA. The values are presented as means ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as *P < 0.05, **P < 0.01, and ***P < 0.001.
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fig5: Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on cell cycle distribution in HepG2 cells. After treatment with ATA at the concentrations of 0, 5, and 10 μg/mL for 48 h, the cells were collected and stained with propidium iodide (PI), with fluorescence intensities measured by flow cytometry. The histogram demonstrated the percentage of cells in various phase in HepG2 cells treated with ATA. The values are presented as means ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as *P < 0.05, **P < 0.01, and ***P < 0.001.

Mentions: After treatment with ATA at 5 and 10 μg/mL for 48 h, the obvious changes in cell-cycle distribution of HepG2 cells were characterized by a decrease of the G0/G1- and S-phase and an increase of the G2/M-phase cells in a dose-dependent manner (P < 0.05, P < 0.01, or P < 0.001), suggesting that ATA suppress cell proliferation associated with cell-cycle arrest in the G2/M phase (Figure 5).


The Inhibitory Effect of 3 β -Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway.

Wang J, Chen X, Zhou Z, Li J, Sun H - Evid Based Complement Alternat Med (2013)

Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on cell cycle distribution in HepG2 cells. After treatment with ATA at the concentrations of 0, 5, and 10 μg/mL for 48 h, the cells were collected and stained with propidium iodide (PI), with fluorescence intensities measured by flow cytometry. The histogram demonstrated the percentage of cells in various phase in HepG2 cells treated with ATA. The values are presented as means ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as *P < 0.05, **P < 0.01, and ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3860155&req=5

fig5: Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on cell cycle distribution in HepG2 cells. After treatment with ATA at the concentrations of 0, 5, and 10 μg/mL for 48 h, the cells were collected and stained with propidium iodide (PI), with fluorescence intensities measured by flow cytometry. The histogram demonstrated the percentage of cells in various phase in HepG2 cells treated with ATA. The values are presented as means ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as *P < 0.05, **P < 0.01, and ***P < 0.001.
Mentions: After treatment with ATA at 5 and 10 μg/mL for 48 h, the obvious changes in cell-cycle distribution of HepG2 cells were characterized by a decrease of the G0/G1- and S-phase and an increase of the G2/M-phase cells in a dose-dependent manner (P < 0.05, P < 0.01, or P < 0.001), suggesting that ATA suppress cell proliferation associated with cell-cycle arrest in the G2/M phase (Figure 5).

Bottom Line: 3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis.PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4.These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis. In this study, we investigated its effects on growth, apoptosis, cell cycle, motility/invasion, and metatasis in human hepatoma HepG2 cells in vitro and antimetastasis of B16-F10 melanoma in mice in vivo, as well as its molecular mechanisms of action using a high-throughput Cancer Pathway Finder PCR Array. ATA could not only induce tumor cells into apoptosis through the activation of both extrinsic and intrinsic pathways, arrest HepG2 cells in G2/M phase, but also suppress the invasion and metastasis abilities of HepG2 cells and the lung metastasis of B16-F10 melanoma in mice. PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4. These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

No MeSH data available.


Related in: MedlinePlus