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The Inhibitory Effect of 3 β -Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway.

Wang J, Chen X, Zhou Z, Li J, Sun H - Evid Based Complement Alternat Med (2013)

Bottom Line: 3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis.PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4.These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis. In this study, we investigated its effects on growth, apoptosis, cell cycle, motility/invasion, and metatasis in human hepatoma HepG2 cells in vitro and antimetastasis of B16-F10 melanoma in mice in vivo, as well as its molecular mechanisms of action using a high-throughput Cancer Pathway Finder PCR Array. ATA could not only induce tumor cells into apoptosis through the activation of both extrinsic and intrinsic pathways, arrest HepG2 cells in G2/M phase, but also suppress the invasion and metastasis abilities of HepG2 cells and the lung metastasis of B16-F10 melanoma in mice. PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4. These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

No MeSH data available.


Related in: MedlinePlus

Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on the viability of HepG2 cells. HepG2 cells were treated with ATA at indicated concentrations (0–20 μg/mL) of for 24, 48, and 72 h, respectively, and the results were expressed by percentages of surviving cells over untreated control cells using MTT assays. The values are presented as mean ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as *P < 0.05 and ***P < 0.001.
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fig2: Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on the viability of HepG2 cells. HepG2 cells were treated with ATA at indicated concentrations (0–20 μg/mL) of for 24, 48, and 72 h, respectively, and the results were expressed by percentages of surviving cells over untreated control cells using MTT assays. The values are presented as mean ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as *P < 0.05 and ***P < 0.001.

Mentions: The cytotoxic effect of ATA on HepG2 cells was determined using MTT assay. As shown in Figure 2, ATA significantly inhibited the growth of HepG2 cell in dose- and time-dependent manners. The morphology of HepG2 cells after 24 and 48 h treatment with ATA was also observed by AO staining under fluorescence microscope, and the results were shown in Figure 3. After staining with AO, the DNA in the nucleus of control cells had homogeneously Kelly fluorescence, while ATA-treated cells showed typical apoptotic features characterized by volume reduction, chromatin condensation, nuclear fragmentation with densely Kelly fluorescence stain, and appearance of apoptotic bodies.


The Inhibitory Effect of 3 β -Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway.

Wang J, Chen X, Zhou Z, Li J, Sun H - Evid Based Complement Alternat Med (2013)

Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on the viability of HepG2 cells. HepG2 cells were treated with ATA at indicated concentrations (0–20 μg/mL) of for 24, 48, and 72 h, respectively, and the results were expressed by percentages of surviving cells over untreated control cells using MTT assays. The values are presented as mean ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as *P < 0.05 and ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3860155&req=5

fig2: Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on the viability of HepG2 cells. HepG2 cells were treated with ATA at indicated concentrations (0–20 μg/mL) of for 24, 48, and 72 h, respectively, and the results were expressed by percentages of surviving cells over untreated control cells using MTT assays. The values are presented as mean ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as *P < 0.05 and ***P < 0.001.
Mentions: The cytotoxic effect of ATA on HepG2 cells was determined using MTT assay. As shown in Figure 2, ATA significantly inhibited the growth of HepG2 cell in dose- and time-dependent manners. The morphology of HepG2 cells after 24 and 48 h treatment with ATA was also observed by AO staining under fluorescence microscope, and the results were shown in Figure 3. After staining with AO, the DNA in the nucleus of control cells had homogeneously Kelly fluorescence, while ATA-treated cells showed typical apoptotic features characterized by volume reduction, chromatin condensation, nuclear fragmentation with densely Kelly fluorescence stain, and appearance of apoptotic bodies.

Bottom Line: 3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis.PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4.These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

ABSTRACT
3 β -Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes of Astilbe chinensis. In this study, we investigated its effects on growth, apoptosis, cell cycle, motility/invasion, and metatasis in human hepatoma HepG2 cells in vitro and antimetastasis of B16-F10 melanoma in mice in vivo, as well as its molecular mechanisms of action using a high-throughput Cancer Pathway Finder PCR Array. ATA could not only induce tumor cells into apoptosis through the activation of both extrinsic and intrinsic pathways, arrest HepG2 cells in G2/M phase, but also suppress the invasion and metastasis abilities of HepG2 cells and the lung metastasis of B16-F10 melanoma in mice. PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4. These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

No MeSH data available.


Related in: MedlinePlus