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Whole body microwave irradiation for improved dacarbazine therapeutical action in cutaneous melanoma mouse model.

Neagu M, Constantin C, Martin D, Albulescu L, Iacob N, Ighigeanu D - Radiol Res Pract (2013)

Bottom Line: Results.Quantifying serum IL-1 β , IL-6, IL-10, IL-12 (p70), IFN- γ , GM-CSF, TNF- α , MIP-1 α , MCP-1, and KC during tumorigenesis and therapy found that the combined experimental therapy decreases all the inflammatory cytokines, except chemokine MCP-1 that was found increased, suggesting an increase of the anti-tumoral immune response triggered by the combined therapy.The overall metastatic process is decreased in the combined therapy group.

View Article: PubMed Central - PubMed

Affiliation: Immunology Department, Immunobiology Laboratory, "Victor Babes" National Institute of Pathology, 99-101 Splaiul Independentei, sector 5, Bucharest 050096, Romania.

ABSTRACT
A cutaneous melanoma mouse model was used to test the efficacy of a new therapeutical approach that uses low doses of cytostatics in conjunction with mild whole body microwave exposure of 2.45 GHz in order to enhance cytostatics antitumoral effect. Materials and Methods. A microwave exposure system for C57BL/6 mouse whole body microwave irradiation was designed; groups of 40 mice (males and females) bearing experimental tumours were subjected to a combined therapy comprising low doses of dacarbazine in combination with mild whole body irradiation. Clinical parameters and serum cytokine testing using xMAP technology were performed. Results. The group that was subjected to combined therapy, microwave and cytostatic, had the best clinical evolution in terms of overall survival, tumour volume, and metastatic potential. At day 14 the untreated group had 100% mortality, while in the combined therapy group 40% of mice were surviving. Quantifying serum IL-1 β , IL-6, IL-10, IL-12 (p70), IFN- γ , GM-CSF, TNF- α , MIP-1 α , MCP-1, and KC during tumorigenesis and therapy found that the combined experimental therapy decreases all the inflammatory cytokines, except chemokine MCP-1 that was found increased, suggesting an increase of the anti-tumoral immune response triggered by the combined therapy. The overall metastatic process is decreased in the combined therapy group.

No MeSH data available.


Related in: MedlinePlus

(a) Schematic drawing of the MEM-MWO; (b) photograph of the MEM-MWO.
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fig1: (a) Schematic drawing of the MEM-MWO; (b) photograph of the MEM-MWO.

Mentions: The MW exposure system (MWES) used for C57BL/6 mouse in a whole body irradiation procedure with microwaves of 2.45 GHz is an innovative and flexible experimental installation (special designed for separate, successive and combined irradiation with MW, and accelerated electron beam) that was previously described and reported in [31]. For better understanding of experiments performed in the frame of this work, we decided to render several features of MWES. It consists mainly of a radiation exposure chamber (REC) and a microwave source of 2.45 GHz with adjustable output power (0–50 W), generated as 10 ms pulses at 50 Hz repetition rate, as REC the multimode rectangular cavity of a proper mechanical and electrical modified MW oven (MEM-MWO) is used. In this installations the conventional operation of 2.45 GHz oven magnetron supplied by an L.C. single-phase-half-wave doublers (L.C. HWD) was modified in order to permit the use of a manually or PC-controlled electronic regulator for the MW power adjustment and remote control [39]. The magnetron main power units consisting of a high voltage diode, a high voltage capacitor, and a high voltage anode transformer (HVAT) are similar to the units used for the conventional magnetron supplying system. Modification consisted in the use of a separate transformer for the filament supply and of a triad controlled regulator added to the HVAT primary circuit. Also, several electronic units are added for MW exposure time presetting as well as for magnetron peak and average current measurement. Another feature added to the MWES operation was obtained by modifying the geometry and rotation velocity of the sample rotary system, as shown in Figures 1(a), 1(b), and 2.


Whole body microwave irradiation for improved dacarbazine therapeutical action in cutaneous melanoma mouse model.

Neagu M, Constantin C, Martin D, Albulescu L, Iacob N, Ighigeanu D - Radiol Res Pract (2013)

(a) Schematic drawing of the MEM-MWO; (b) photograph of the MEM-MWO.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3860147&req=5

fig1: (a) Schematic drawing of the MEM-MWO; (b) photograph of the MEM-MWO.
Mentions: The MW exposure system (MWES) used for C57BL/6 mouse in a whole body irradiation procedure with microwaves of 2.45 GHz is an innovative and flexible experimental installation (special designed for separate, successive and combined irradiation with MW, and accelerated electron beam) that was previously described and reported in [31]. For better understanding of experiments performed in the frame of this work, we decided to render several features of MWES. It consists mainly of a radiation exposure chamber (REC) and a microwave source of 2.45 GHz with adjustable output power (0–50 W), generated as 10 ms pulses at 50 Hz repetition rate, as REC the multimode rectangular cavity of a proper mechanical and electrical modified MW oven (MEM-MWO) is used. In this installations the conventional operation of 2.45 GHz oven magnetron supplied by an L.C. single-phase-half-wave doublers (L.C. HWD) was modified in order to permit the use of a manually or PC-controlled electronic regulator for the MW power adjustment and remote control [39]. The magnetron main power units consisting of a high voltage diode, a high voltage capacitor, and a high voltage anode transformer (HVAT) are similar to the units used for the conventional magnetron supplying system. Modification consisted in the use of a separate transformer for the filament supply and of a triad controlled regulator added to the HVAT primary circuit. Also, several electronic units are added for MW exposure time presetting as well as for magnetron peak and average current measurement. Another feature added to the MWES operation was obtained by modifying the geometry and rotation velocity of the sample rotary system, as shown in Figures 1(a), 1(b), and 2.

Bottom Line: Results.Quantifying serum IL-1 β , IL-6, IL-10, IL-12 (p70), IFN- γ , GM-CSF, TNF- α , MIP-1 α , MCP-1, and KC during tumorigenesis and therapy found that the combined experimental therapy decreases all the inflammatory cytokines, except chemokine MCP-1 that was found increased, suggesting an increase of the anti-tumoral immune response triggered by the combined therapy.The overall metastatic process is decreased in the combined therapy group.

View Article: PubMed Central - PubMed

Affiliation: Immunology Department, Immunobiology Laboratory, "Victor Babes" National Institute of Pathology, 99-101 Splaiul Independentei, sector 5, Bucharest 050096, Romania.

ABSTRACT
A cutaneous melanoma mouse model was used to test the efficacy of a new therapeutical approach that uses low doses of cytostatics in conjunction with mild whole body microwave exposure of 2.45 GHz in order to enhance cytostatics antitumoral effect. Materials and Methods. A microwave exposure system for C57BL/6 mouse whole body microwave irradiation was designed; groups of 40 mice (males and females) bearing experimental tumours were subjected to a combined therapy comprising low doses of dacarbazine in combination with mild whole body irradiation. Clinical parameters and serum cytokine testing using xMAP technology were performed. Results. The group that was subjected to combined therapy, microwave and cytostatic, had the best clinical evolution in terms of overall survival, tumour volume, and metastatic potential. At day 14 the untreated group had 100% mortality, while in the combined therapy group 40% of mice were surviving. Quantifying serum IL-1 β , IL-6, IL-10, IL-12 (p70), IFN- γ , GM-CSF, TNF- α , MIP-1 α , MCP-1, and KC during tumorigenesis and therapy found that the combined experimental therapy decreases all the inflammatory cytokines, except chemokine MCP-1 that was found increased, suggesting an increase of the anti-tumoral immune response triggered by the combined therapy. The overall metastatic process is decreased in the combined therapy group.

No MeSH data available.


Related in: MedlinePlus