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Effects of Lipophilic Extract of Viscum album L. and Oleanolic Acid on Migratory Activity of NIH/3T3 Fibroblasts and on HaCat Keratinocytes.

Kuonen R, Weissenstein U, Urech K, Kunz M, Hostanska K, Estko M, Heusser P, Baumgartner S - Evid Based Complement Alternat Med (2013)

Bottom Line: At higher concentrations both substances affected proliferation and viability of NIH/3T3 fibroblasts and HaCat keratinocytes.In the toxic range of concentrations of VALE and OA, migration of NIH/3T3 fibroblasts was suppressed.The extent of the stimulatory effect on cell migration of VALE quite closely corresponded to the effect expected by the concentrations of OA contained in the crude extract VALE.

View Article: PubMed Central - PubMed

Affiliation: Society for Cancer Research, Hiscia Institute, 4144 Arlesheim, Switzerland.

ABSTRACT
Viscum album L. lipophilic extract (VALE) contains pharmacologically active pentacyclic triterpenes that are known to exhibit immunomodulatory, antitumor, and wound healing activity. Preliminary clinical observations indicate that VALE was able to influence cutaneous wound healing in vivo. The objective of this study was to investigate wound closure related properties of VALE in vitro. As measured in a wound healing assay, VALE and its predominant triterpene oleanolic acid (OA) significantly and dose dependently promoted the migration of NIH/3T3 fibroblasts in vitro, thereby leading to an enhanced wound closure. Compared to the negative control, maximal stimulation by 26.1% and 26.2%, respectively, was attained with 10 μg/mL VALE and 1 μg/mL OA. Stimulation of proliferation in NIH/3T3 fibroblasts by VALE and OA could be excluded. At higher concentrations both substances affected proliferation and viability of NIH/3T3 fibroblasts and HaCat keratinocytes. In the toxic range of concentrations of VALE and OA, migration of NIH/3T3 fibroblasts was suppressed. The extent of the stimulatory effect on cell migration of VALE quite closely corresponded to the effect expected by the concentrations of OA contained in the crude extract VALE. These data support the casual observation that Viscum album L. lipophilic extract might modulate wound healing related processes in vivo.

No MeSH data available.


Related in: MedlinePlus

Dose-dependent cytotoxic effect of VALE on (a) NIH/3T3 and (b) HaCat cells and of OA on (c) NIH/3T3 and (d) HaCat cells after 24 h and 48 h of incubation. Mean values ± SE of three experiments are expressed in percentage of cell viability compared to the untreated control.
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Related In: Results  -  Collection


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fig1: Dose-dependent cytotoxic effect of VALE on (a) NIH/3T3 and (b) HaCat cells and of OA on (c) NIH/3T3 and (d) HaCat cells after 24 h and 48 h of incubation. Mean values ± SE of three experiments are expressed in percentage of cell viability compared to the untreated control.

Mentions: VALE investigated at concentrations from 25 to 2000 μg/mL in NIH/3T3 cells and from 100 to 1600 μg/mL in HaCat cells decreased the viability of both cell lines in a dose-dependent manner (Figures 1(a) and 1(b)). NIH/3T3 cells were slightly more sensitive to VALE than HaCat cells.


Effects of Lipophilic Extract of Viscum album L. and Oleanolic Acid on Migratory Activity of NIH/3T3 Fibroblasts and on HaCat Keratinocytes.

Kuonen R, Weissenstein U, Urech K, Kunz M, Hostanska K, Estko M, Heusser P, Baumgartner S - Evid Based Complement Alternat Med (2013)

Dose-dependent cytotoxic effect of VALE on (a) NIH/3T3 and (b) HaCat cells and of OA on (c) NIH/3T3 and (d) HaCat cells after 24 h and 48 h of incubation. Mean values ± SE of three experiments are expressed in percentage of cell viability compared to the untreated control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3860131&req=5

fig1: Dose-dependent cytotoxic effect of VALE on (a) NIH/3T3 and (b) HaCat cells and of OA on (c) NIH/3T3 and (d) HaCat cells after 24 h and 48 h of incubation. Mean values ± SE of three experiments are expressed in percentage of cell viability compared to the untreated control.
Mentions: VALE investigated at concentrations from 25 to 2000 μg/mL in NIH/3T3 cells and from 100 to 1600 μg/mL in HaCat cells decreased the viability of both cell lines in a dose-dependent manner (Figures 1(a) and 1(b)). NIH/3T3 cells were slightly more sensitive to VALE than HaCat cells.

Bottom Line: At higher concentrations both substances affected proliferation and viability of NIH/3T3 fibroblasts and HaCat keratinocytes.In the toxic range of concentrations of VALE and OA, migration of NIH/3T3 fibroblasts was suppressed.The extent of the stimulatory effect on cell migration of VALE quite closely corresponded to the effect expected by the concentrations of OA contained in the crude extract VALE.

View Article: PubMed Central - PubMed

Affiliation: Society for Cancer Research, Hiscia Institute, 4144 Arlesheim, Switzerland.

ABSTRACT
Viscum album L. lipophilic extract (VALE) contains pharmacologically active pentacyclic triterpenes that are known to exhibit immunomodulatory, antitumor, and wound healing activity. Preliminary clinical observations indicate that VALE was able to influence cutaneous wound healing in vivo. The objective of this study was to investigate wound closure related properties of VALE in vitro. As measured in a wound healing assay, VALE and its predominant triterpene oleanolic acid (OA) significantly and dose dependently promoted the migration of NIH/3T3 fibroblasts in vitro, thereby leading to an enhanced wound closure. Compared to the negative control, maximal stimulation by 26.1% and 26.2%, respectively, was attained with 10 μg/mL VALE and 1 μg/mL OA. Stimulation of proliferation in NIH/3T3 fibroblasts by VALE and OA could be excluded. At higher concentrations both substances affected proliferation and viability of NIH/3T3 fibroblasts and HaCat keratinocytes. In the toxic range of concentrations of VALE and OA, migration of NIH/3T3 fibroblasts was suppressed. The extent of the stimulatory effect on cell migration of VALE quite closely corresponded to the effect expected by the concentrations of OA contained in the crude extract VALE. These data support the casual observation that Viscum album L. lipophilic extract might modulate wound healing related processes in vivo.

No MeSH data available.


Related in: MedlinePlus