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Expression and Localization of Neuregulin-1 (Nrg1) and ErbB2/ErbB4 Receptors in Main Endocrine Organs of the Rhesus Monkey.

Zhao WJ - Int J Endocrinol Metab (2013)

Bottom Line: The expression level of Nrg1 on each sample was indexed by the fold of integrative fluorescence intensity (IFI) relative to that of one cortical tissue.Differential expression of Nrg1 and their cognate receptors ErbB2 and ErbB4 were found selectively expressed in endocrine organs we tested, with higher expression levels detected in the adrenal gland (AG) and pancreas.In the pituitary, adjacent localization of Nrg1 positive cells with ErbB4 positive cells were observed.

View Article: PubMed Central - PubMed

Affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, People's Republic of China.

ABSTRACT

Background: Although Neuregulin-1 (Nrg1)and its receptors have been indicated at the mRNA level in partial human endocrine organs and its functional roles have been evaluated in vitro, their morphological distribution in higher animals are not fully studied. The present research focused on expression of Nrg1 and its main receptors ErbB2 and ErbB4 in main endocrine organs of the rhesus monkey.

Materials and methods: The morphological expression of Nrg1 and its receptors ErbB2 and ErbB4 as well as their potential co-localization were determined by double immunofluorescence in the pituitary, thyroid, parathyroid, pancreas and adrenal gland sample tissues. The expression level of Nrg1 on each sample was indexed by the fold of integrative fluorescence intensity (IFI) relative to that of one cortical tissue.

Results: Differential expression of Nrg1 and their cognate receptors ErbB2 and ErbB4 were found selectively expressed in endocrine organs we tested, with higher expression levels detected in the adrenal gland (AG) and pancreas. Co-localization of Nrg1 with either ErbB2 or ErbB4 was detected in AG, thyroid and parathyroid gland, and Nrg1 was only co-localized with ErbB4 in the islet cells of the pancreas. In the pituitary, adjacent localization of Nrg1 positive cells with ErbB4 positive cells were observed.

Conclusions: This investigation morphologically profiles the differential expression of Nrg1 and its receptors ErbB2 and ErbB4 in the main endocrine organ structures, suggesting an autocrine or paracrine-directed Nrg1-ErbB signaling pathway in some of these structures.

No MeSH data available.


Related in: MedlinePlus

Expression and Localization of Nrg1, as well as ErbB2/ErbB4 in the Parathyroid of the Rhesus Monkey.(a) H & E staining. (b-i) Double Immunofluorescence Staining for Nrg1 with either ErbB2 (b-e) or ErbB4 (f-i) was shown. Scale bars = 50μm.
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fig3756: Expression and Localization of Nrg1, as well as ErbB2/ErbB4 in the Parathyroid of the Rhesus Monkey.(a) H & E staining. (b-i) Double Immunofluorescence Staining for Nrg1 with either ErbB2 (b-e) or ErbB4 (f-i) was shown. Scale bars = 50μm.

Mentions: Basic parathyroid structures based on HE staining was demonstrated in Figure 4a. The larger polygonal oxyphil cells, which constitute a smaller population, showed positive staining of Nrg1 (Figure 4e, g). In addition, co-localization of Nrg1 with both ErbB2 (Figure 4b-e) and ErbB4 (Figure 4f-i) was found mainly in these cells.


Expression and Localization of Neuregulin-1 (Nrg1) and ErbB2/ErbB4 Receptors in Main Endocrine Organs of the Rhesus Monkey.

Zhao WJ - Int J Endocrinol Metab (2013)

Expression and Localization of Nrg1, as well as ErbB2/ErbB4 in the Parathyroid of the Rhesus Monkey.(a) H & E staining. (b-i) Double Immunofluorescence Staining for Nrg1 with either ErbB2 (b-e) or ErbB4 (f-i) was shown. Scale bars = 50μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860111&req=5

fig3756: Expression and Localization of Nrg1, as well as ErbB2/ErbB4 in the Parathyroid of the Rhesus Monkey.(a) H & E staining. (b-i) Double Immunofluorescence Staining for Nrg1 with either ErbB2 (b-e) or ErbB4 (f-i) was shown. Scale bars = 50μm.
Mentions: Basic parathyroid structures based on HE staining was demonstrated in Figure 4a. The larger polygonal oxyphil cells, which constitute a smaller population, showed positive staining of Nrg1 (Figure 4e, g). In addition, co-localization of Nrg1 with both ErbB2 (Figure 4b-e) and ErbB4 (Figure 4f-i) was found mainly in these cells.

Bottom Line: The expression level of Nrg1 on each sample was indexed by the fold of integrative fluorescence intensity (IFI) relative to that of one cortical tissue.Differential expression of Nrg1 and their cognate receptors ErbB2 and ErbB4 were found selectively expressed in endocrine organs we tested, with higher expression levels detected in the adrenal gland (AG) and pancreas.In the pituitary, adjacent localization of Nrg1 positive cells with ErbB4 positive cells were observed.

View Article: PubMed Central - PubMed

Affiliation: Center for Neuroscience, Shantou University Medical College, Shantou, People's Republic of China.

ABSTRACT

Background: Although Neuregulin-1 (Nrg1)and its receptors have been indicated at the mRNA level in partial human endocrine organs and its functional roles have been evaluated in vitro, their morphological distribution in higher animals are not fully studied. The present research focused on expression of Nrg1 and its main receptors ErbB2 and ErbB4 in main endocrine organs of the rhesus monkey.

Materials and methods: The morphological expression of Nrg1 and its receptors ErbB2 and ErbB4 as well as their potential co-localization were determined by double immunofluorescence in the pituitary, thyroid, parathyroid, pancreas and adrenal gland sample tissues. The expression level of Nrg1 on each sample was indexed by the fold of integrative fluorescence intensity (IFI) relative to that of one cortical tissue.

Results: Differential expression of Nrg1 and their cognate receptors ErbB2 and ErbB4 were found selectively expressed in endocrine organs we tested, with higher expression levels detected in the adrenal gland (AG) and pancreas. Co-localization of Nrg1 with either ErbB2 or ErbB4 was detected in AG, thyroid and parathyroid gland, and Nrg1 was only co-localized with ErbB4 in the islet cells of the pancreas. In the pituitary, adjacent localization of Nrg1 positive cells with ErbB4 positive cells were observed.

Conclusions: This investigation morphologically profiles the differential expression of Nrg1 and its receptors ErbB2 and ErbB4 in the main endocrine organ structures, suggesting an autocrine or paracrine-directed Nrg1-ErbB signaling pathway in some of these structures.

No MeSH data available.


Related in: MedlinePlus