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Liraglutide Improves the Survival of INS-1 Cells by Promoting Macroautophagy.

Jing Yin J, Bo Li Y, Ming Cao M, Wang Y - Int J Endocrinol Metab (2013)

Bottom Line: Liraglutide, which has many special anti-diabetes biological effects, is found to inhibit beta cell death and ameliorate endoplasmic reticulum stress (ERs) induced by free fatty acid (FFA).Apoptosis induced by PA in INS-1 cells was significantly resolved after Liraglutide treatment.These findings provide a novel role for GLP-1 analogue in preventing or treating with T2D.

View Article: PubMed Central - PubMed

Affiliation: The Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

ABSTRACT

Background: Type 2 diabetes mellitus (T2D) is a metabolic disease characterized by dysfunction of pancreatic beta cell and insulin resistance. Liraglutide, which has many special anti-diabetes biological effects, is found to inhibit beta cell death and ameliorate endoplasmic reticulum stress (ERs) induced by free fatty acid (FFA). Macroautophagy (hereafter referred to as autophagy) altered by FFA is also associated with the dysfunction or death of pancreatic beta cells.

Objectives: We aim at proving that Liraglutide improves the survival of INS-1 cells by promoting autophagy.

Materials and methods: Cell survival was assessed by CCK8 assay. The percentage of apoptotic cells was determined by flow cytometric assay after Annexin V-FITC/PI staining. Expression of LC3 was detected by western blotting. MDC staining and transmission electron microscopy (TEM) were used in the measurement of autophagy.

Results: Apoptosis induced by PA in INS-1 cells was significantly resolved after Liraglutide treatment. Simultaneously, autophagy was enhanced with the treatment of PA and Liraglutide.

Conclusions: Liraglutide appears to protect INS-1 cells from apoptosis FFA-induced by promoting autophagy.

Conclusions: These findings provide a novel role for GLP-1 analogue in preventing or treating with T2D.

No MeSH data available.


Related in: MedlinePlus

Liraglutide Induced Autophgy in INS-1 Cells Loaded with PA.The INS-1 cells treated with 0.5 mmol l–1 PA for indicated time durations were stained with 50 um MDC for 10 min. The fluorescent dots were observed under a fluorescence microscope. (a) control; (b) palmitate 0.5 mmol l–1; (c) Liraglutide 100 nmol l–1 ; (d) palmitate 0.5 mmol l–1 and Liraglutide 100 nmol l–1 .
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fig3952: Liraglutide Induced Autophgy in INS-1 Cells Loaded with PA.The INS-1 cells treated with 0.5 mmol l–1 PA for indicated time durations were stained with 50 um MDC for 10 min. The fluorescent dots were observed under a fluorescence microscope. (a) control; (b) palmitate 0.5 mmol l–1; (c) Liraglutide 100 nmol l–1 ; (d) palmitate 0.5 mmol l–1 and Liraglutide 100 nmol l–1 .

Mentions: When MDC, an autofluorescent compound used for the in vivo labeling of autophagic vacuoles, was applied to PA-treated INS-1 cells with Liraglutide, the proportion of cells with MDC stained dots was dramatically increased (Figure 3).


Liraglutide Improves the Survival of INS-1 Cells by Promoting Macroautophagy.

Jing Yin J, Bo Li Y, Ming Cao M, Wang Y - Int J Endocrinol Metab (2013)

Liraglutide Induced Autophgy in INS-1 Cells Loaded with PA.The INS-1 cells treated with 0.5 mmol l–1 PA for indicated time durations were stained with 50 um MDC for 10 min. The fluorescent dots were observed under a fluorescence microscope. (a) control; (b) palmitate 0.5 mmol l–1; (c) Liraglutide 100 nmol l–1 ; (d) palmitate 0.5 mmol l–1 and Liraglutide 100 nmol l–1 .
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860108&req=5

fig3952: Liraglutide Induced Autophgy in INS-1 Cells Loaded with PA.The INS-1 cells treated with 0.5 mmol l–1 PA for indicated time durations were stained with 50 um MDC for 10 min. The fluorescent dots were observed under a fluorescence microscope. (a) control; (b) palmitate 0.5 mmol l–1; (c) Liraglutide 100 nmol l–1 ; (d) palmitate 0.5 mmol l–1 and Liraglutide 100 nmol l–1 .
Mentions: When MDC, an autofluorescent compound used for the in vivo labeling of autophagic vacuoles, was applied to PA-treated INS-1 cells with Liraglutide, the proportion of cells with MDC stained dots was dramatically increased (Figure 3).

Bottom Line: Liraglutide, which has many special anti-diabetes biological effects, is found to inhibit beta cell death and ameliorate endoplasmic reticulum stress (ERs) induced by free fatty acid (FFA).Apoptosis induced by PA in INS-1 cells was significantly resolved after Liraglutide treatment.These findings provide a novel role for GLP-1 analogue in preventing or treating with T2D.

View Article: PubMed Central - PubMed

Affiliation: The Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

ABSTRACT

Background: Type 2 diabetes mellitus (T2D) is a metabolic disease characterized by dysfunction of pancreatic beta cell and insulin resistance. Liraglutide, which has many special anti-diabetes biological effects, is found to inhibit beta cell death and ameliorate endoplasmic reticulum stress (ERs) induced by free fatty acid (FFA). Macroautophagy (hereafter referred to as autophagy) altered by FFA is also associated with the dysfunction or death of pancreatic beta cells.

Objectives: We aim at proving that Liraglutide improves the survival of INS-1 cells by promoting autophagy.

Materials and methods: Cell survival was assessed by CCK8 assay. The percentage of apoptotic cells was determined by flow cytometric assay after Annexin V-FITC/PI staining. Expression of LC3 was detected by western blotting. MDC staining and transmission electron microscopy (TEM) were used in the measurement of autophagy.

Results: Apoptosis induced by PA in INS-1 cells was significantly resolved after Liraglutide treatment. Simultaneously, autophagy was enhanced with the treatment of PA and Liraglutide.

Conclusions: Liraglutide appears to protect INS-1 cells from apoptosis FFA-induced by promoting autophagy.

Conclusions: These findings provide a novel role for GLP-1 analogue in preventing or treating with T2D.

No MeSH data available.


Related in: MedlinePlus