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Liraglutide Improves the Survival of INS-1 Cells by Promoting Macroautophagy.

Jing Yin J, Bo Li Y, Ming Cao M, Wang Y - Int J Endocrinol Metab (2013)

Bottom Line: Liraglutide, which has many special anti-diabetes biological effects, is found to inhibit beta cell death and ameliorate endoplasmic reticulum stress (ERs) induced by free fatty acid (FFA).Apoptosis induced by PA in INS-1 cells was significantly resolved after Liraglutide treatment.These findings provide a novel role for GLP-1 analogue in preventing or treating with T2D.

View Article: PubMed Central - PubMed

Affiliation: The Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

ABSTRACT

Background: Type 2 diabetes mellitus (T2D) is a metabolic disease characterized by dysfunction of pancreatic beta cell and insulin resistance. Liraglutide, which has many special anti-diabetes biological effects, is found to inhibit beta cell death and ameliorate endoplasmic reticulum stress (ERs) induced by free fatty acid (FFA). Macroautophagy (hereafter referred to as autophagy) altered by FFA is also associated with the dysfunction or death of pancreatic beta cells.

Objectives: We aim at proving that Liraglutide improves the survival of INS-1 cells by promoting autophagy.

Materials and methods: Cell survival was assessed by CCK8 assay. The percentage of apoptotic cells was determined by flow cytometric assay after Annexin V-FITC/PI staining. Expression of LC3 was detected by western blotting. MDC staining and transmission electron microscopy (TEM) were used in the measurement of autophagy.

Results: Apoptosis induced by PA in INS-1 cells was significantly resolved after Liraglutide treatment. Simultaneously, autophagy was enhanced with the treatment of PA and Liraglutide.

Conclusions: Liraglutide appears to protect INS-1 cells from apoptosis FFA-induced by promoting autophagy.

Conclusions: These findings provide a novel role for GLP-1 analogue in preventing or treating with T2D.

No MeSH data available.


Related in: MedlinePlus

Liraglutide inhibits FFA-induced apoptosis in INS-1 cells.INS-1 cells were pre-treated with Liraglutide (100 nmol l–1) for 24h, then exposed to palmitate (PA, 0.5 mmol l–1) for 24h. The rate of apoptosis was determined by flow cytometric assay after Annexin V-FITC/PI staining. (a) control; (b) PA alone; (c) Liraglutide alone; (d) PA+Liraglutide; (e) 3-MA; (f) PA+3-MA;
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fig3969: Liraglutide inhibits FFA-induced apoptosis in INS-1 cells.INS-1 cells were pre-treated with Liraglutide (100 nmol l–1) for 24h, then exposed to palmitate (PA, 0.5 mmol l–1) for 24h. The rate of apoptosis was determined by flow cytometric assay after Annexin V-FITC/PI staining. (a) control; (b) PA alone; (c) Liraglutide alone; (d) PA+Liraglutide; (e) 3-MA; (f) PA+3-MA;

Mentions: An Annexin V-FITC/PI quantification assay demonstrated that PA-induced INS-1 cells were mediated by apoptosis. In our study, the apoptosis of INS-1 cells increased to 19% induced by PA compared with 5% in control group (Figure 2b). In addition, as shown in Figure 2d, apoptosis proportion was reduced to 13% suggesting a protective role of Liraglutide in INS-1 cells form PA-induced apoptosis. Autophagy inhibitor, 3-MA, produced an increased apoptosis to 10% in 3-MA group (Figure 2e) and 26% in PA+3-MA group (Figure 2f).


Liraglutide Improves the Survival of INS-1 Cells by Promoting Macroautophagy.

Jing Yin J, Bo Li Y, Ming Cao M, Wang Y - Int J Endocrinol Metab (2013)

Liraglutide inhibits FFA-induced apoptosis in INS-1 cells.INS-1 cells were pre-treated with Liraglutide (100 nmol l–1) for 24h, then exposed to palmitate (PA, 0.5 mmol l–1) for 24h. The rate of apoptosis was determined by flow cytometric assay after Annexin V-FITC/PI staining. (a) control; (b) PA alone; (c) Liraglutide alone; (d) PA+Liraglutide; (e) 3-MA; (f) PA+3-MA;
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860108&req=5

fig3969: Liraglutide inhibits FFA-induced apoptosis in INS-1 cells.INS-1 cells were pre-treated with Liraglutide (100 nmol l–1) for 24h, then exposed to palmitate (PA, 0.5 mmol l–1) for 24h. The rate of apoptosis was determined by flow cytometric assay after Annexin V-FITC/PI staining. (a) control; (b) PA alone; (c) Liraglutide alone; (d) PA+Liraglutide; (e) 3-MA; (f) PA+3-MA;
Mentions: An Annexin V-FITC/PI quantification assay demonstrated that PA-induced INS-1 cells were mediated by apoptosis. In our study, the apoptosis of INS-1 cells increased to 19% induced by PA compared with 5% in control group (Figure 2b). In addition, as shown in Figure 2d, apoptosis proportion was reduced to 13% suggesting a protective role of Liraglutide in INS-1 cells form PA-induced apoptosis. Autophagy inhibitor, 3-MA, produced an increased apoptosis to 10% in 3-MA group (Figure 2e) and 26% in PA+3-MA group (Figure 2f).

Bottom Line: Liraglutide, which has many special anti-diabetes biological effects, is found to inhibit beta cell death and ameliorate endoplasmic reticulum stress (ERs) induced by free fatty acid (FFA).Apoptosis induced by PA in INS-1 cells was significantly resolved after Liraglutide treatment.These findings provide a novel role for GLP-1 analogue in preventing or treating with T2D.

View Article: PubMed Central - PubMed

Affiliation: The Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

ABSTRACT

Background: Type 2 diabetes mellitus (T2D) is a metabolic disease characterized by dysfunction of pancreatic beta cell and insulin resistance. Liraglutide, which has many special anti-diabetes biological effects, is found to inhibit beta cell death and ameliorate endoplasmic reticulum stress (ERs) induced by free fatty acid (FFA). Macroautophagy (hereafter referred to as autophagy) altered by FFA is also associated with the dysfunction or death of pancreatic beta cells.

Objectives: We aim at proving that Liraglutide improves the survival of INS-1 cells by promoting autophagy.

Materials and methods: Cell survival was assessed by CCK8 assay. The percentage of apoptotic cells was determined by flow cytometric assay after Annexin V-FITC/PI staining. Expression of LC3 was detected by western blotting. MDC staining and transmission electron microscopy (TEM) were used in the measurement of autophagy.

Results: Apoptosis induced by PA in INS-1 cells was significantly resolved after Liraglutide treatment. Simultaneously, autophagy was enhanced with the treatment of PA and Liraglutide.

Conclusions: Liraglutide appears to protect INS-1 cells from apoptosis FFA-induced by promoting autophagy.

Conclusions: These findings provide a novel role for GLP-1 analogue in preventing or treating with T2D.

No MeSH data available.


Related in: MedlinePlus