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Expression profile of IL-35 mRNA in gingiva of chronic periodontitis and aggressive periodontitis patients: a semiquantitative RT-PCR study.

Kalburgi NB, Muley A, Shivaprasad BM, Koregol AC - Dis. Markers (2013)

Bottom Line: The present study demonstrated the expression of IL-35 mRNA in gingival tissues of all the three groups.The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis.Future studies done on large samples with intervention will strengthen our result.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, PMNM Dental College, Bagalkot, Karnataka 587101, India.

ABSTRACT

Background: Proinflammatory and anti-inflammatory cytokines play a key role in the pathogenesis of periodontal diseases. Secretion of bioactive IL-35 has been described by T regulatory cells (T(regs)) and is required for their maximal suppressive activity. T(regs) are involved in the modulation of local immune response in chronic periodontitis patients.

Objective: Hence, the present study was aimed to investigate the expression of IL-35 mRNA in chronic periodontitis and aggressive periodontitis patients.

Materials and methods: The present study was carried out in 60 subjects, which included 20 chronic periodontitis patients, 20 aggressive periodontitis patients, and 20 periodontally healthy controls. IL-35 mRNA expression in gingival tissue samples of all subjects was semiquantitatively analyzed using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR).

Results: The present study demonstrated the expression of IL-35 mRNA in gingival tissues of all the three groups. IL-35 mRNA expression was highest in chronic periodontitis subjects (6.87 ± 2.32) as compared to the aggressive periodontitis group (4.71 ± 1.43) and least seen in healthy patients (3.03 ± 1.91).

Conclusion: The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis. Future studies done on large samples with intervention will strengthen our result.

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Related in: MedlinePlus

Distribution of study samples according to sex.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig2: Distribution of study samples according to sex.

Mentions: The various clinical parameters as well as IL-35m RNA levels were analyzed in gingival tissue samples for all the 3 groups. Mean age of subjects were 24.40 ± 5.01 years in healthy group, 44.80 ± 7.62 years in chronic periodontitis group, and 27.90 ± 4.54 years in aggressive periodontitis group. All the three groups were gender matched (Figures 1 and 2).


Expression profile of IL-35 mRNA in gingiva of chronic periodontitis and aggressive periodontitis patients: a semiquantitative RT-PCR study.

Kalburgi NB, Muley A, Shivaprasad BM, Koregol AC - Dis. Markers (2013)

Distribution of study samples according to sex.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3860092&req=5

fig2: Distribution of study samples according to sex.
Mentions: The various clinical parameters as well as IL-35m RNA levels were analyzed in gingival tissue samples for all the 3 groups. Mean age of subjects were 24.40 ± 5.01 years in healthy group, 44.80 ± 7.62 years in chronic periodontitis group, and 27.90 ± 4.54 years in aggressive periodontitis group. All the three groups were gender matched (Figures 1 and 2).

Bottom Line: The present study demonstrated the expression of IL-35 mRNA in gingival tissues of all the three groups.The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis.Future studies done on large samples with intervention will strengthen our result.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, PMNM Dental College, Bagalkot, Karnataka 587101, India.

ABSTRACT

Background: Proinflammatory and anti-inflammatory cytokines play a key role in the pathogenesis of periodontal diseases. Secretion of bioactive IL-35 has been described by T regulatory cells (T(regs)) and is required for their maximal suppressive activity. T(regs) are involved in the modulation of local immune response in chronic periodontitis patients.

Objective: Hence, the present study was aimed to investigate the expression of IL-35 mRNA in chronic periodontitis and aggressive periodontitis patients.

Materials and methods: The present study was carried out in 60 subjects, which included 20 chronic periodontitis patients, 20 aggressive periodontitis patients, and 20 periodontally healthy controls. IL-35 mRNA expression in gingival tissue samples of all subjects was semiquantitatively analyzed using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR).

Results: The present study demonstrated the expression of IL-35 mRNA in gingival tissues of all the three groups. IL-35 mRNA expression was highest in chronic periodontitis subjects (6.87 ± 2.32) as compared to the aggressive periodontitis group (4.71 ± 1.43) and least seen in healthy patients (3.03 ± 1.91).

Conclusion: The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis. Future studies done on large samples with intervention will strengthen our result.

Show MeSH
Related in: MedlinePlus