Lineweaver-Burk plot of ACE activity in the presence of

Characterization of an antihypertensive angiotensin I-converting enzyme inhibitory peptide from the edible mushroom Hypsizygus marmoreus. Kang MG, Kim YH, Bolormaa Z, Kim MK, Seo GS, Lee JS - Biomed Res Int (2013) Bottom Line: Therefore, the development of new antihypertensive drugs or bioactive compounds is very important to remedy or prevent hypertension.The purified ACE inhibitor was found to be a new oligopeptide with the sequence LSMGSASLSP.Its molecular weight was estimated to be 567.3 Da and the water extracts containing ACE inhibitor from Hypsizygus marmoreus showed a clear antihypertensive action a spontaneously hypertensive rat. View Article: PubMed Central - PubMed Affiliation: Department of Biomedicinal Science and Biotechnology, PaiChai University, Daejeon 302-735, Republic of Korea. ABSTRACT Hypertension is one of the very serious diseases and, recently, hypertensive patient longevity has been increased significantly. Therefore, the development of new antihypertensive drugs or bioactive compounds is very important to remedy or prevent hypertension. The antihypertensive angiotensin I-converting enzyme (ACE) inhibitor in water extracts from the brown-cultivar-fruiting-body of Hypsizygus marmoreus was purified with ultrafiltration, C18 solid phase extraction chromatography and reverse-phase HPLC, and the purified ACE inhibitor with inhibitory activity of IC50 value of 0.19 mg/mL was obtained. The purified ACE inhibitor was found to be a new oligopeptide with the sequence LSMGSASLSP. Its molecular weight was estimated to be 567.3 Da and the water extracts containing ACE inhibitor from Hypsizygus marmoreus showed a clear antihypertensive action a spontaneously hypertensive rat. Show MeSH Major Angiotensin-Converting Enzyme Inhibitors/administration & dosage/chemistry/isolation & purification Antihypertensive Agents/administration & dosage/chemistry Hypertension/drug therapy/pathology Peptides/administration & dosage/chemistry/isolation & purification Minor Agaricales/enzymology Animals Blood Pressure/drug effects Chromatography, High Pressure Liquid Humans Peptidyl-Dipeptidase A/metabolism Rats Rats, Inbred SHR Related in: MedlinePlus	© Copyright Policy Related In: Results - Collection License Show All Figures getmorefigures.php?uid=PMC3860087&req=5 fig5: Lineweaver-Burk plot of ACE activity in the presence of the purified ACE inhibitor (P-2). (▲; Control, ■; 0.1 mg of inhibitor, ×; 0.3 mg of inhibitor). Mentions: The inhibitory pattern of the purified ACE inhibitor P-2 was investigated using a Lineweaver-Burk plot (Figure 5). It was found that the purified ACE inhibitor had a noncompetitive inhibitory pattern, unlike those of Grifola frondosa [7] and Tricholoma giganteum [9], which showed patterns of competitive inhibition.

Characterization of an antihypertensive angiotensin I-converting enzyme inhibitory peptide from the edible mushroom Hypsizygus marmoreus.

Kang MG, Kim YH, Bolormaa Z, Kim MK, Seo GS, Lee JS - Biomed Res Int (2013)

Bottom Line: Therefore, the development of new antihypertensive drugs or bioactive compounds is very important to remedy or prevent hypertension.The purified ACE inhibitor was found to be a new oligopeptide with the sequence LSMGSASLSP.Its molecular weight was estimated to be 567.3 Da and the water extracts containing ACE inhibitor from Hypsizygus marmoreus showed a clear antihypertensive action a spontaneously hypertensive rat.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedicinal Science and Biotechnology, PaiChai University, Daejeon 302-735, Republic of Korea.

ABSTRACT

Hypertension is one of the very serious diseases and, recently, hypertensive patient longevity has been increased significantly. Therefore, the development of new antihypertensive drugs or bioactive compounds is very important to remedy or prevent hypertension. The antihypertensive angiotensin I-converting enzyme (ACE) inhibitor in water extracts from the brown-cultivar-fruiting-body of Hypsizygus marmoreus was purified with ultrafiltration, C18 solid phase extraction chromatography and reverse-phase HPLC, and the purified ACE inhibitor with inhibitory activity of IC50 value of 0.19 mg/mL was obtained. The purified ACE inhibitor was found to be a new oligopeptide with the sequence LSMGSASLSP. Its molecular weight was estimated to be 567.3 Da and the water extracts containing ACE inhibitor from Hypsizygus marmoreus showed a clear antihypertensive action a spontaneously hypertensive rat.

Show MeSH

Related in: MedlinePlus

Lineweaver-Burk plot of ACE activity in the presence of the purified ACE inhibitor (P-2). (▲; Control, ■; 0.1 mg of inhibitor, ×; 0.3 mg of inhibitor).

Related In: Results - Collection

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fig5: Lineweaver-Burk plot of ACE activity in the presence of the purified ACE inhibitor (P-2). (▲; Control, ■; 0.1 mg of inhibitor, ×; 0.3 mg of inhibitor).

Mentions: The inhibitory pattern of the purified ACE inhibitor P-2 was investigated using a Lineweaver-Burk plot (Figure 5). It was found that the purified ACE inhibitor had a noncompetitive inhibitory pattern, unlike those of Grifola frondosa [7] and Tricholoma giganteum [9], which showed patterns of competitive inhibition.