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Characterization of an antihypertensive angiotensin I-converting enzyme inhibitory peptide from the edible mushroom Hypsizygus marmoreus.

Kang MG, Kim YH, Bolormaa Z, Kim MK, Seo GS, Lee JS - Biomed Res Int (2013)

Bottom Line: Therefore, the development of new antihypertensive drugs or bioactive compounds is very important to remedy or prevent hypertension.The purified ACE inhibitor was found to be a new oligopeptide with the sequence LSMGSASLSP.Its molecular weight was estimated to be 567.3 Da and the water extracts containing ACE inhibitor from Hypsizygus marmoreus showed a clear antihypertensive action a spontaneously hypertensive rat.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedicinal Science and Biotechnology, PaiChai University, Daejeon 302-735, Republic of Korea.

ABSTRACT
Hypertension is one of the very serious diseases and, recently, hypertensive patient longevity has been increased significantly. Therefore, the development of new antihypertensive drugs or bioactive compounds is very important to remedy or prevent hypertension. The antihypertensive angiotensin I-converting enzyme (ACE) inhibitor in water extracts from the brown-cultivar-fruiting-body of Hypsizygus marmoreus was purified with ultrafiltration, C18 solid phase extraction chromatography and reverse-phase HPLC, and the purified ACE inhibitor with inhibitory activity of IC50 value of 0.19 mg/mL was obtained. The purified ACE inhibitor was found to be a new oligopeptide with the sequence LSMGSASLSP. Its molecular weight was estimated to be 567.3 Da and the water extracts containing ACE inhibitor from Hypsizygus marmoreus showed a clear antihypertensive action a spontaneously hypertensive rat.

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Related in: MedlinePlus

RP-HPLC chromatogram on l Vydac protein/peptide reverse-phase 218TP column of active fraction F1-2-3 (50 mM). Separation was performed with a linear gradient of acetonitrile containing 0.1% TFA from 5% to 25% at a flow rate of 1 mL/min.
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fig1: RP-HPLC chromatogram on l Vydac protein/peptide reverse-phase 218TP column of active fraction F1-2-3 (50 mM). Separation was performed with a linear gradient of acetonitrile containing 0.1% TFA from 5% to 25% at a flow rate of 1 mL/min.

Mentions: After C18 solid-phase extraction from 5% to 100% using acetonitrile, the fraction F1-2 from the extraction with 25% acetonitrile showed high IC50: 0.57 mg/mL of ACE inhibitory activity. After SCX solid-phase extraction of the active fraction by 10–200 mM of ammonium formate, the active fraction (F1-2-1) from an extraction of 50 mM ammonium formate, 0.36 mg/mL of IC50 was obtained (data not shown). Subsequently, RP-HPLCs were performed on the active fraction the using a l-Vydac-protein/peptide reverse-phase 218TP column and a purified ACE inhibitor showing ACE inhibitory activity (IC50) of 0.34 mg/mL was obtained (Figure 1).


Characterization of an antihypertensive angiotensin I-converting enzyme inhibitory peptide from the edible mushroom Hypsizygus marmoreus.

Kang MG, Kim YH, Bolormaa Z, Kim MK, Seo GS, Lee JS - Biomed Res Int (2013)

RP-HPLC chromatogram on l Vydac protein/peptide reverse-phase 218TP column of active fraction F1-2-3 (50 mM). Separation was performed with a linear gradient of acetonitrile containing 0.1% TFA from 5% to 25% at a flow rate of 1 mL/min.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860087&req=5

fig1: RP-HPLC chromatogram on l Vydac protein/peptide reverse-phase 218TP column of active fraction F1-2-3 (50 mM). Separation was performed with a linear gradient of acetonitrile containing 0.1% TFA from 5% to 25% at a flow rate of 1 mL/min.
Mentions: After C18 solid-phase extraction from 5% to 100% using acetonitrile, the fraction F1-2 from the extraction with 25% acetonitrile showed high IC50: 0.57 mg/mL of ACE inhibitory activity. After SCX solid-phase extraction of the active fraction by 10–200 mM of ammonium formate, the active fraction (F1-2-1) from an extraction of 50 mM ammonium formate, 0.36 mg/mL of IC50 was obtained (data not shown). Subsequently, RP-HPLCs were performed on the active fraction the using a l-Vydac-protein/peptide reverse-phase 218TP column and a purified ACE inhibitor showing ACE inhibitory activity (IC50) of 0.34 mg/mL was obtained (Figure 1).

Bottom Line: Therefore, the development of new antihypertensive drugs or bioactive compounds is very important to remedy or prevent hypertension.The purified ACE inhibitor was found to be a new oligopeptide with the sequence LSMGSASLSP.Its molecular weight was estimated to be 567.3 Da and the water extracts containing ACE inhibitor from Hypsizygus marmoreus showed a clear antihypertensive action a spontaneously hypertensive rat.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedicinal Science and Biotechnology, PaiChai University, Daejeon 302-735, Republic of Korea.

ABSTRACT
Hypertension is one of the very serious diseases and, recently, hypertensive patient longevity has been increased significantly. Therefore, the development of new antihypertensive drugs or bioactive compounds is very important to remedy or prevent hypertension. The antihypertensive angiotensin I-converting enzyme (ACE) inhibitor in water extracts from the brown-cultivar-fruiting-body of Hypsizygus marmoreus was purified with ultrafiltration, C18 solid phase extraction chromatography and reverse-phase HPLC, and the purified ACE inhibitor with inhibitory activity of IC50 value of 0.19 mg/mL was obtained. The purified ACE inhibitor was found to be a new oligopeptide with the sequence LSMGSASLSP. Its molecular weight was estimated to be 567.3 Da and the water extracts containing ACE inhibitor from Hypsizygus marmoreus showed a clear antihypertensive action a spontaneously hypertensive rat.

Show MeSH
Related in: MedlinePlus