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The study on biocompatibility of porous nHA/PLGA composite scaffolds for tissue engineering with rabbit chondrocytes in vitro.

Chen L, Zhu WM, Fei ZQ, Chen JL, Xiong JY, Zhang JF, Duan L, Huang J, Liu Z, Wang D, Zeng Y - Biomed Res Int (2013)

Bottom Line: Cells on the surface and in the pores of the scaffold increased in a time-dependent manner.Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05).The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Guangzhou Medical College, Guangzhou, Guangdong 510182, China ; Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.

ABSTRACT

Objective: To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA) composite and evaluate its feasibility as a scaffold for cartilage tissue engineering.

Methods: Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM). The cell cycle profile was analyzed by flow cytometry.

Results: The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05).

Conclusion: The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

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Related in: MedlinePlus

Microscopic images of cells of control group A and experimental group B at different time points (100x). After 1 day in culture, cell density in group A (a) and group B (b) was low. The cells were observed triangle-shaped, disc-shaped, and megagon-shaped. After 3 days in culture, the cells in group A (c) and group B (d) were observed to connect by cellular processes. After 5 days in culture, cells in group A (f) and group B (e) increased and completely covered the surface of the composite.
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fig4: Microscopic images of cells of control group A and experimental group B at different time points (100x). After 1 day in culture, cell density in group A (a) and group B (b) was low. The cells were observed triangle-shaped, disc-shaped, and megagon-shaped. After 3 days in culture, the cells in group A (c) and group B (d) were observed to connect by cellular processes. After 5 days in culture, cells in group A (f) and group B (e) increased and completely covered the surface of the composite.

Mentions: Chondrocytes in group A and group B were imaged and analyzed by inverted microscope (Figures 4(a)–4(f)). In both groups, the chondrocytes were observed triangle-shaped, disc-shaped, and megagon-shaped. The cells were connected by cellular processes. The chondrocytes observed kept proliferating and differentiating during culture.


The study on biocompatibility of porous nHA/PLGA composite scaffolds for tissue engineering with rabbit chondrocytes in vitro.

Chen L, Zhu WM, Fei ZQ, Chen JL, Xiong JY, Zhang JF, Duan L, Huang J, Liu Z, Wang D, Zeng Y - Biomed Res Int (2013)

Microscopic images of cells of control group A and experimental group B at different time points (100x). After 1 day in culture, cell density in group A (a) and group B (b) was low. The cells were observed triangle-shaped, disc-shaped, and megagon-shaped. After 3 days in culture, the cells in group A (c) and group B (d) were observed to connect by cellular processes. After 5 days in culture, cells in group A (f) and group B (e) increased and completely covered the surface of the composite.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860085&req=5

fig4: Microscopic images of cells of control group A and experimental group B at different time points (100x). After 1 day in culture, cell density in group A (a) and group B (b) was low. The cells were observed triangle-shaped, disc-shaped, and megagon-shaped. After 3 days in culture, the cells in group A (c) and group B (d) were observed to connect by cellular processes. After 5 days in culture, cells in group A (f) and group B (e) increased and completely covered the surface of the composite.
Mentions: Chondrocytes in group A and group B were imaged and analyzed by inverted microscope (Figures 4(a)–4(f)). In both groups, the chondrocytes were observed triangle-shaped, disc-shaped, and megagon-shaped. The cells were connected by cellular processes. The chondrocytes observed kept proliferating and differentiating during culture.

Bottom Line: Cells on the surface and in the pores of the scaffold increased in a time-dependent manner.Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05).The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Guangzhou Medical College, Guangzhou, Guangdong 510182, China ; Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.

ABSTRACT

Objective: To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA) composite and evaluate its feasibility as a scaffold for cartilage tissue engineering.

Methods: Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM). The cell cycle profile was analyzed by flow cytometry.

Results: The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05).

Conclusion: The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

Show MeSH
Related in: MedlinePlus