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The study on biocompatibility of porous nHA/PLGA composite scaffolds for tissue engineering with rabbit chondrocytes in vitro.

Chen L, Zhu WM, Fei ZQ, Chen JL, Xiong JY, Zhang JF, Duan L, Huang J, Liu Z, Wang D, Zeng Y - Biomed Res Int (2013)

Bottom Line: Cells on the surface and in the pores of the scaffold increased in a time-dependent manner.Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05).The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Guangzhou Medical College, Guangzhou, Guangdong 510182, China ; Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.

ABSTRACT

Objective: To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA) composite and evaluate its feasibility as a scaffold for cartilage tissue engineering.

Methods: Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM). The cell cycle profile was analyzed by flow cytometry.

Results: The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05).

Conclusion: The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

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SEM images of the porous PLGA/n-HA composites. (a) SEM image of the three-dimensional porous PLGA/n-HA composite (50x). The outside diameter is around 0.6 mm. (b) SEM image of the internal structure of the PLGA/n-HA composite (1000x). The pore diameter ranges from 100 μm to 300 μm and the porosity reaches up to 90%.
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fig2: SEM images of the porous PLGA/n-HA composites. (a) SEM image of the three-dimensional porous PLGA/n-HA composite (50x). The outside diameter is around 0.6 mm. (b) SEM image of the internal structure of the PLGA/n-HA composite (1000x). The pore diameter ranges from 100 μm to 300 μm and the porosity reaches up to 90%.

Mentions: The porous nHA/PLGA composite Scaffold is 20 mm × 20 mm × 20 mm (Figure 1) in size. The porosity was measured by alcohol soaking method and average porosity was 85%~90%. The pore size of the scaffold was measured by scanning electron microscopy (Sem), ranging from 100 to 300 microns (Figure 2(b)).


The study on biocompatibility of porous nHA/PLGA composite scaffolds for tissue engineering with rabbit chondrocytes in vitro.

Chen L, Zhu WM, Fei ZQ, Chen JL, Xiong JY, Zhang JF, Duan L, Huang J, Liu Z, Wang D, Zeng Y - Biomed Res Int (2013)

SEM images of the porous PLGA/n-HA composites. (a) SEM image of the three-dimensional porous PLGA/n-HA composite (50x). The outside diameter is around 0.6 mm. (b) SEM image of the internal structure of the PLGA/n-HA composite (1000x). The pore diameter ranges from 100 μm to 300 μm and the porosity reaches up to 90%.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC3860085&req=5

fig2: SEM images of the porous PLGA/n-HA composites. (a) SEM image of the three-dimensional porous PLGA/n-HA composite (50x). The outside diameter is around 0.6 mm. (b) SEM image of the internal structure of the PLGA/n-HA composite (1000x). The pore diameter ranges from 100 μm to 300 μm and the porosity reaches up to 90%.
Mentions: The porous nHA/PLGA composite Scaffold is 20 mm × 20 mm × 20 mm (Figure 1) in size. The porosity was measured by alcohol soaking method and average porosity was 85%~90%. The pore size of the scaffold was measured by scanning electron microscopy (Sem), ranging from 100 to 300 microns (Figure 2(b)).

Bottom Line: Cells on the surface and in the pores of the scaffold increased in a time-dependent manner.Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05).The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Guangzhou Medical College, Guangzhou, Guangdong 510182, China ; Department of Orthopedics, Second People's Hospital of Shenzhen, Sungang West Road, Futian District, Shenzhen, Guangdong 518035, China ; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen, Guangdong 518035, China.

ABSTRACT

Objective: To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA) composite and evaluate its feasibility as a scaffold for cartilage tissue engineering.

Methods: Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM). The cell cycle profile was analyzed by flow cytometry.

Results: The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P > 0.05).

Conclusion: The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

Show MeSH