Limits...
Suitability of cell-based label-free detection for cytotoxicity screening of carbon nanotubes.

Meindl C, Absenger M, Roblegg E, Fröhlich E - Biomed Res Int (2013)

Bottom Line: For validation of the label-free systems different concentrations of ethanol and of amine (AMI) polystyrene NPs were used.All systems identified thin (<8 nm) CNTs as more cytotoxic than thick (>20 nm) CNTs, but detection by xCELLigence system was less sensitive to CNT-induced cytotoxicity.Despite advantages, such as continuous monitoring and more detailed analysis of cytotoxic effects, label-free techniques cannot be generally recommended for cytotoxicity screening of NPs.

View Article: PubMed Central - PubMed

Affiliation: Center for Medical Research, Medical University of Graz, 8010 Graz, Austria.

ABSTRACT
Cytotoxicity testing of nanoparticles (NPs) by conventional screening assays is often complicated by interference. Carbon nanotubes (CNTs) are particularly difficult to assess. To test the suitability of cell-based label-free techniques for this application, a panel of CNTs with different diameters and surface functionalizations was assessed by impedance-based technique (xCELLigence RTCA) and automated microscopy (Cell-IQ) compared to formazan bioreduction (MTS assay). For validation of the label-free systems different concentrations of ethanol and of amine (AMI) polystyrene NPs were used. CNTs were evaluated in various cell lines, but only endothelial EAhy926 cells and L929 and V79 fibroblasts could be evaluated in all systems. Polystyrene particles obtained similar results in all assays. All systems identified thin (<8 nm) CNTs as more cytotoxic than thick (>20 nm) CNTs, but detection by xCELLigence system was less sensitive to CNT-induced cytotoxicity. Despite advantages, such as continuous monitoring and more detailed analysis of cytotoxic effects, label-free techniques cannot be generally recommended for cytotoxicity screening of NPs.

Show MeSH
Fraction of stable cells according to Cell-IQ analysis in different cell lines exposed to CNTs (n = 4). Untreated cells are set as 100%. Significant changes are marked by asterisk.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3860081&req=5

fig6: Fraction of stable cells according to Cell-IQ analysis in different cell lines exposed to CNTs (n = 4). Untreated cells are set as 100%. Significant changes are marked by asterisk.

Mentions: Stable cells after incubation of EAhy926 cells with all CNTs for 4 h at a concentration of 100 μg/mL were significantly decreased (Figure 6). In L929 cells, SCNTc and MCNT8c caused significant decreases after 4 h, while decreases for SCNT, MCNT8, and MCNT20c were significant only after 24 h. A transient decrease in viability after 24 h was observed after incubation with MCNT50. Viability of V79 was decreased for incubations with all CNTs, except MCNT20, after 4 h.


Suitability of cell-based label-free detection for cytotoxicity screening of carbon nanotubes.

Meindl C, Absenger M, Roblegg E, Fröhlich E - Biomed Res Int (2013)

Fraction of stable cells according to Cell-IQ analysis in different cell lines exposed to CNTs (n = 4). Untreated cells are set as 100%. Significant changes are marked by asterisk.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860081&req=5

fig6: Fraction of stable cells according to Cell-IQ analysis in different cell lines exposed to CNTs (n = 4). Untreated cells are set as 100%. Significant changes are marked by asterisk.
Mentions: Stable cells after incubation of EAhy926 cells with all CNTs for 4 h at a concentration of 100 μg/mL were significantly decreased (Figure 6). In L929 cells, SCNTc and MCNT8c caused significant decreases after 4 h, while decreases for SCNT, MCNT8, and MCNT20c were significant only after 24 h. A transient decrease in viability after 24 h was observed after incubation with MCNT50. Viability of V79 was decreased for incubations with all CNTs, except MCNT20, after 4 h.

Bottom Line: For validation of the label-free systems different concentrations of ethanol and of amine (AMI) polystyrene NPs were used.All systems identified thin (<8 nm) CNTs as more cytotoxic than thick (>20 nm) CNTs, but detection by xCELLigence system was less sensitive to CNT-induced cytotoxicity.Despite advantages, such as continuous monitoring and more detailed analysis of cytotoxic effects, label-free techniques cannot be generally recommended for cytotoxicity screening of NPs.

View Article: PubMed Central - PubMed

Affiliation: Center for Medical Research, Medical University of Graz, 8010 Graz, Austria.

ABSTRACT
Cytotoxicity testing of nanoparticles (NPs) by conventional screening assays is often complicated by interference. Carbon nanotubes (CNTs) are particularly difficult to assess. To test the suitability of cell-based label-free techniques for this application, a panel of CNTs with different diameters and surface functionalizations was assessed by impedance-based technique (xCELLigence RTCA) and automated microscopy (Cell-IQ) compared to formazan bioreduction (MTS assay). For validation of the label-free systems different concentrations of ethanol and of amine (AMI) polystyrene NPs were used. CNTs were evaluated in various cell lines, but only endothelial EAhy926 cells and L929 and V79 fibroblasts could be evaluated in all systems. Polystyrene particles obtained similar results in all assays. All systems identified thin (<8 nm) CNTs as more cytotoxic than thick (>20 nm) CNTs, but detection by xCELLigence system was less sensitive to CNT-induced cytotoxicity. Despite advantages, such as continuous monitoring and more detailed analysis of cytotoxic effects, label-free techniques cannot be generally recommended for cytotoxicity screening of NPs.

Show MeSH