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Infrared laser pulse triggers increased singlet oxygen production in tumour cells.

Sokolovski SG, Zolotovskaya SA, Goltsov A, Pourreyron C, South AP, Rafailov EU - Sci Rep (2013)

Bottom Line: This technique utilises singlet oxygen ((1)O2) generation via a laser excited photosensitiser (PS) to kill cancer cells.However, prolonged sensitivity to intensive light (6-8 weeks for lung cancer), relatively low tissue penetration by activating light (630 nm up to 4 mm), and the cost of PS administration can limit progressive PDT applications.Our modelling and experimental results support the development of direct infrared (IR) laser-induced tumour treatment as a promising approach in tumour PDT.

View Article: PubMed Central - PubMed

Affiliation: Photonics and Nanoscience Group, School of Engineering, Physics and Mathematics, University of Dundee, Dundee DD1 4HN, UK.

ABSTRACT
Photodynamic therapy (PDT) is a technique developed to treat the ever-increasing global incidence of cancer. This technique utilises singlet oxygen ((1)O2) generation via a laser excited photosensitiser (PS) to kill cancer cells. However, prolonged sensitivity to intensive light (6-8 weeks for lung cancer), relatively low tissue penetration by activating light (630 nm up to 4 mm), and the cost of PS administration can limit progressive PDT applications. The development of quantum-dot laser diodes emitting in the highest absorption region (1268 nm) of triplet oxygen ((3)O2) presents the possibility of inducing apoptosis in tumour cells through direct (3)O2 → (1)O2 transition. Here we demonstrate that a single laser pulse triggers dose-dependent (1)O2 generation in both normal keratinocytes and tumour cells and show that tumour cells yield the highest (1)O2 far beyond the initial laser pulse exposure. Our modelling and experimental results support the development of direct infrared (IR) laser-induced tumour treatment as a promising approach in tumour PDT.

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Related in: MedlinePlus

A model of cell redox homeostasis and its imbalance by laser-induced ROS generation.(A) Scheme of cellular ROS production and scavenging. (B) Kinetics of ROS in normal and (C) cancerous cells. H2O2 (); reduced PSH (); primary ROS (1O2 and O2−), R1, (); reduced thioredoxin peroxidase, Px (); sum of primary and secondary ROS, R2 (); rate of 1O2 generation by 3 min laser pulse only ().
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f3: A model of cell redox homeostasis and its imbalance by laser-induced ROS generation.(A) Scheme of cellular ROS production and scavenging. (B) Kinetics of ROS in normal and (C) cancerous cells. H2O2 (); reduced PSH (); primary ROS (1O2 and O2−), R1, (); reduced thioredoxin peroxidase, Px (); sum of primary and secondary ROS, R2 (); rate of 1O2 generation by 3 min laser pulse only ().

Mentions: For comprehensive analysis of our results, a kinetic model of redox homeostasis and its imbalance by laser-induced ROS generation in normal and cancerous cells was developed (Fig. 3 and 4A).


Infrared laser pulse triggers increased singlet oxygen production in tumour cells.

Sokolovski SG, Zolotovskaya SA, Goltsov A, Pourreyron C, South AP, Rafailov EU - Sci Rep (2013)

A model of cell redox homeostasis and its imbalance by laser-induced ROS generation.(A) Scheme of cellular ROS production and scavenging. (B) Kinetics of ROS in normal and (C) cancerous cells. H2O2 (); reduced PSH (); primary ROS (1O2 and O2−), R1, (); reduced thioredoxin peroxidase, Px (); sum of primary and secondary ROS, R2 (); rate of 1O2 generation by 3 min laser pulse only ().
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3860013&req=5

f3: A model of cell redox homeostasis and its imbalance by laser-induced ROS generation.(A) Scheme of cellular ROS production and scavenging. (B) Kinetics of ROS in normal and (C) cancerous cells. H2O2 (); reduced PSH (); primary ROS (1O2 and O2−), R1, (); reduced thioredoxin peroxidase, Px (); sum of primary and secondary ROS, R2 (); rate of 1O2 generation by 3 min laser pulse only ().
Mentions: For comprehensive analysis of our results, a kinetic model of redox homeostasis and its imbalance by laser-induced ROS generation in normal and cancerous cells was developed (Fig. 3 and 4A).

Bottom Line: This technique utilises singlet oxygen ((1)O2) generation via a laser excited photosensitiser (PS) to kill cancer cells.However, prolonged sensitivity to intensive light (6-8 weeks for lung cancer), relatively low tissue penetration by activating light (630 nm up to 4 mm), and the cost of PS administration can limit progressive PDT applications.Our modelling and experimental results support the development of direct infrared (IR) laser-induced tumour treatment as a promising approach in tumour PDT.

View Article: PubMed Central - PubMed

Affiliation: Photonics and Nanoscience Group, School of Engineering, Physics and Mathematics, University of Dundee, Dundee DD1 4HN, UK.

ABSTRACT
Photodynamic therapy (PDT) is a technique developed to treat the ever-increasing global incidence of cancer. This technique utilises singlet oxygen ((1)O2) generation via a laser excited photosensitiser (PS) to kill cancer cells. However, prolonged sensitivity to intensive light (6-8 weeks for lung cancer), relatively low tissue penetration by activating light (630 nm up to 4 mm), and the cost of PS administration can limit progressive PDT applications. The development of quantum-dot laser diodes emitting in the highest absorption region (1268 nm) of triplet oxygen ((3)O2) presents the possibility of inducing apoptosis in tumour cells through direct (3)O2 → (1)O2 transition. Here we demonstrate that a single laser pulse triggers dose-dependent (1)O2 generation in both normal keratinocytes and tumour cells and show that tumour cells yield the highest (1)O2 far beyond the initial laser pulse exposure. Our modelling and experimental results support the development of direct infrared (IR) laser-induced tumour treatment as a promising approach in tumour PDT.

Show MeSH
Related in: MedlinePlus